LOCUS       sequence-113017-         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombination-Induced Tag Exchange (RITE) Cassette, T7 to an HA-GFP 
            tag, Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Menendez-Benito V, van Deventer SJ, Jimenez-Garcia V, Roy-Luzarraga 
            M, van Leeuwen F, Neefjes J
  TITLE     Spatiotemporal analysis of organelle and macromolecular complex 
            inheritance.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Jan 2;110(1):175-80. doi: 
            10.1073/pnas.1207424110. Epub 2012 Dec 17.
  PUBMED    23248297
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1207424110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-01-2- 2"; volume: "110"; issue: "1"; pages: "175-80"
COMMENT     Sequence Label: pVM013
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        8..26
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(41..757)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(44..757)
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(764..790)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(799..832)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 agctcggaat taaccctcac taaagggaac aaaagctttt ttatttatac aattcatcca
       61 taccatgggt aataccagca gcagtaacaa attctaacaa gaccatgtgg tctctctttt
      121 cgtttggatc tttggataag gcagattgag tggataagta atggttgtct ggtaacaaga
      181 ctggaccatc accaattgga gtattttgtt gataatggtc agctaattga acagaaccat
      241 cttcaatgtt gtgtctaatt ttgaagttaa ctttgatacc attcttttgt ttgtcagcca
      301 tgatgtaaac attgtgagag ttatagttgt attccaattt gtgacctaaa atgttaccat
      361 cttctttaaa atcaatacct tttaattcga ttctattaac taaggtatca ccttcaaact
      421 tgacttcagc tctggtcttg tagttaccgt catctttgaa aaaaatagtt ctttcttgaa
      481 cataaccttc tggcatggca gacttgaaaa agtcatgttg tttcatatga tctgggtatc
      541 tagaaaaaca ttgaacacca taagttaaag tagtgactaa ggttggccat ggaactggca
      601 atttaccagt agtacaaata aattttaagg tcaatttacc gtaagtagca tcaccttcac
      661 cttcaccgga gacagaaaat ttgtgaccat taacatcacc atctaattca accaaaattg
      721 ggacaacacc agtgaataat tcttcacctt tagacattgt acacgcatag tcaggaacat
      781 cgtatgggta gtcgactgat aacttcgtat agcatacatt atacgaagtt attctagact
      841 gtgcggtatt tcacaccgca tagggttaat taaggcgcgc cagatctgtt tagcttgcct
      901 cgtccccgcc gggtcacccg gccagcgaca tggaggccca gaataccctc cttgacagtc
      961 ttgacgtgcg cagctcaggg gcatgatgtg actgtc
//
