LOCUS       sequence-113018-         652 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of GFP in mammalian cells. Control vector for 
            FUW-ubiquitin-EphB-SV40-GFP vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Cortina C, Palomo-Ponce S, Iglesias M, Fernandez-Masip JL, Vivancos 
            A, Whissell G, Huma M, Peiro N, Gallego L, Jonkheer S, Davy A, 
            Lloreta J, Sancho E, Batlle E
  TITLE     EphB-ephrin-B interactions suppress colorectal cancer progression by
            compartmentalizing tumor cells.
  JOURNAL   Nat Genet. 2007 Nov;39(11):1376-83. Epub 2007 Sep 30.
  PUBMED    17906625
REFERENCE   2  (bases 1 to 652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Genet."; date: "2007-11"; volume: "39(11)"; pages: "1376-83. Epub 
            2007 Sep 30"
COMMENT     Sequence Label: FUW-ubiquitin-SV40-GFP
FEATURES             Location/Qualifiers
     source          1..652
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      5..14
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(25..235)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      31..166
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(36..333)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        183..374
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     331..351
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 ctcaccatgg tggctagctg caaaagccta ggcctccaaa aaagcctcct cactacttct
       61 ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc
      121 catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg
      181 gcgggactat ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg
      241 agcctgggga ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc
      301 tgcctgctgg ggagcctggg gactttccac accctaactg acacacattc cacagctggt
      361 tctttccgcc tcagaaggta cctaaccaag ttcctctttc agaggttatt tcaggccatg
      421 gtgctgcgca ccctgcttgc ccatactata tgttttaatt tatatttttt ctttccccct
      481 ggccttaacc gaattttttc ccatcgcgat ctaattctcc cccgcttaat actgacgctc
      541 tcgcacccat ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt actcaccagt
      601 cgccgcccct cgcctcttgc cgtgcgcgct tcagcaagcc gagtcctgcg tc
//
