LOCUS       sequence-113019-       14085 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast expression plasmid: ACC1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14085)
  AUTHORS   Shi S, Chen Y, Siewers V, Nielsen J
  TITLE     Improving production of malonyl coenzyme A-derived metabolites by 
            abolishing Snf1-dependent regulation of Acc1.
  JOURNAL   MBio. 2014 May 6;5(3):e01130-14. doi: 10.1128/mBio.01130-14.
  PUBMED    24803522
REFERENCE   2  (bases 1 to 14085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/mBio"; 
            journalName: "MBio"; date: "2014-05-6- 6"; volume: "5"; issue: "3"; 
            pages: "e01130-14"
COMMENT     Sequence Label: pAW
FEATURES             Location/Qualifiers
     source          1..14085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        196..416
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             417..1220
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(1351..1806)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1488..1509)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1700..1719
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(1911..2076)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     1997..2021
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(3379..3390)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        9944..10350
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     primer_bind     10370..10389
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10370..10388
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             10406..10435
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      10465..10654
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(10753..10770)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10924..11512)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11004..11023)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11683..12474,12475..12543))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12306..12325
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12544..12648)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      12675..14017
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(13638..13685)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     14058..14076
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
      241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
      301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc
      361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt
      421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat
      481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca
      541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg
      601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg
      661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca
      721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac
      781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa
      841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg
      901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct
      961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac
     1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg
     1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa
     1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa
     1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac
     1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac
     1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat
     1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     1801 gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     1861 ttcgctatta cgccagctgg ataaaggcgc gccaaacgac ctaggaattg gagcgacctc
     1921 atgctatacc tgagaaagca acctgaccta caggaaagag ttactcaaga ataagaattt
     1981 tcgttttaaa acctaagagt cactttaaaa tttgtataca cttatttttt ttataactta
     2041 tttaataata aaaatcataa atcataagaa attcgcttat ttagaagtgt caacaacgta
     2101 tctaccaacg atttgaccct tttccatctt ttcgtaaatt tctggcaagg tagacaagcc
     2161 gacaaccttg attggagact tgaccaaacc tctggcgaag aattgttaat taagagctcg
     2221 caaggtttat ttcaaagtct tcaacaattt ttctttatca tcggtagata acatcttgat
     2281 aacttcagat aatccatcaa tagcattgtc atggtcgctt ctgatctttt tagctaagtc
     2341 ttgagcgaat gactctaatt tcaaaccctt tagtttatcg tccaaagttt tgtagttttc
     2401 ttcaatccat gttgcgactt gcctatcatc ttcatggtcc actgaagcag ggtaccacga
     2461 tctaattctt gcgatctttt ctaatcttga tgcttcgcct acctgatggc tcaacctttt
     2521 aatcaaatat tcttcgttca atcttcttct caatctccag aagaagaaac gacgtgcctc
     2581 ggtccattcc agttccttag aaataacacc cttggccacc atacgtgaag acctatcgtg
     2641 caaatcagca aattgaagac tgatttgtcc gtaaattggc aatagttctc tctcacgatc
     2701 agctaattgc ttggatattt gctgatgtac ttctggagcc aaactcttgt tggataattg
     2761 agatctcaat tctctgtact tgtcatccaa tctgttcatg gtgtccagca atttttctct
     2821 acggaacttg ataccaacca taccttgtgg ttccaaaaca ccagctctag cgttgacgtc
     2881 ggcatacatt tccatttggt cagcgttgat agttggatcg acaacaaccc atgaaccacc
     2941 tcttagttca ccggtaggtg ggatatagat aataattggt tgtttgtaat ccaccaatgc
     3001 gtcaacaata aacgaaccat acttcaagac ttcgttgaac atatcacgtt gaccaccaga
     3061 gaaacctctc cagttggcca aaatcatcat tggcaattgt tcaccgttgt taaagtcatt
     3121 gatagcttga gcagtcttga aggcggagtt tggatgccaa acttgaccag gttcttgaat
     3181 taatgtttca gcactatttg gattagctgg atcagcagga atcaagttct cgacagttct
     3241 tgtttcaaca ccaataacac ccagtggaat accaccaaga cgggctctac caacgacaac
     3301 acctttggcc catcctgaca aagtttcaaa gaaagaccct ttatcaaaca aaccatattc
     3361 aaatccactt tcagtctcac gaccttcaat catccatctt acatcgtaag tttcatcatt
     3421 agttggagtg aaatcaactg gtctatccca tgtgtcttta gtttccaaga taggaactgg
     3481 catattacgc ttggctggaa cataagacat ccattcaaca atcttctcta caccagctaa
     3541 atcgtcaaca gcagtcaaat gtgaaacacc gttgttatac atgatttgag taccacccaa
     3601 ttgtaagtta gaagtataaa cttctctacc cagcattttg ttgattgcag gagcaccagt
     3661 taaaataatt ggctggcctt cgacctgaat agctctttga cccaaacgaa ccaaataagc
     3721 accgataccg acggatctac aagtgactaa ggtgatagtg aagatatcgt ggtaagccct
     3781 tgacgttgca ccagcaatta aaccagatcc acgtagacat tcgacaccta acccatcttc
     3841 agaaccaata attgtcttga tgacaaatct ttcttcaccg tttataacag tacgttcagt
     3901 gagaacagaa ttttctttgt caaatttctt taaagtttcc ataccttcac ttgttaagta
     3961 taagtattgg aagcccttgt ccggattggc agcatcattc catgcaactt gaaatagtgg
     4021 aacaatctct tcagccatac caattctggc acctgagttt gcagccaagt aaattcttgg
     4081 gataccacgc tttctagcat attcagtaac cttattgaag aattcgtctt cttgtggacc
     4141 aaaggaaccg atcttgaatg tgatatcgtt agcaacaaca acaaattgac ggcctcttgg
     4201 atattcagga gtctttacag taatcttaaa ggcaaccata ccaatagcgt tggcaccagg
     4261 ttctctttcc acctcagtta attcgccgtt ttcatcttca atcaactcgt tggaaataaa
     4321 gaaatcatct gttaacttaa catctgcaga gaaatttttc cattgggatg acgatgcttg
     4381 gcggaataat tctgggaagt catagacata tgtggtaccc atcaagtgtg ccttataacg
     4441 ttttggttgc aaccattcct taacagggta aggagtagca ataggtctta aatgcatgga
     4501 tccaggttta cccaaagact taaataccca ttcacctttt gcgttcttga cttcggtgta
     4561 catttctgtt ttgataacat aaccagaaac gttattgatc aaggcacgca atggtactgg
     4621 ggcacctgtt tgaggatctt tgatgatgat tctaatttcg gcagaagaaa cacgcaatct
     4681 caacaatctc ttaccaaatc tttctaagaa accaccgaag gcggcttcga catcttctgg
     4741 agagatatca aacaccgcaa tgaagttgat gaagatatga ttcaaatcag aatttgaagt
     4801 gtcggtgact tctaaattat ccaatatatc actcatcaat ctgttagctt cagaagtcag
     4861 atattcttga atagaaatgt catcacggat atgacccgtt ctaataatac ctcttgtaaa
     4921 gaatctctta tccaatggag aagtcttact aacagcttcg tagacatgga tgtttctatt
     4981 atcagtgaaa attggtttaa tgttgaagtt ggacaatctt cctaattcca gttggaaggc
     5041 caaagccggc tcaatgtgac gaattgtttc attttcgtta taatttggac cgttaaaagt
     5101 ataatacttt ggataagacc catctttaaa accgaacata aatgtgatac gacggataga
     5161 agcattgatt aattcctgct tattcaaatc caaaatttct ctcaacctta ccaaaatttc
     5221 ctcttcagat tcgaaacctt ctgtagaagc aacacaaaca ttagcaacat tactcaacga
     5281 tgcggagcta ccagaacgat caggagcagg tccgttagaa gaagattggt gacgaggaat
     5341 aacttccaaa ctttgtgaca aaatttcatc aacatcatct aaatgatcca cagccatcaa
     5401 aataccttct cttaacggag atgactgact gtttgcaaca tatgacaaat ctgaaacaga
     5461 aacagccctg ttcataccca ttttagattt aacagttgga aaggtggaga acgcagctga
     5521 aggtagttgg aatttccatt caacaattgg aactgtgaca ccttcgtgaa ctctaatatc
     5581 tcctatggtg taagcacgat aagcacgacg aatatagact tgagcagctg cagcagtcac
     5641 aactgggtct tgatgggtta ggaattgaag taaaacatcg aacacaacgt aattagaatc
     5701 gatcaagtcc ttcaagatat tcaaatctgg ttcagagcgc tttggattgg atgagccata
     5761 ggcaaccttc acaacagagg attttaagat atgttcaatt tgttcagttc tttccttgac
     5821 cgaaggtaaa gcgccttgaa tcaaaatttc tcttgcttgt agagcgacct tagcggtagc
     5881 cttagattct agttcaacaa tatgttgtag aggagtagag aaaatggcag aaactttaga
     5941 agataacttg cacaatggtt gataatgttt caagatagct aggatcaggt tattcttcgc
     6001 tgaaactttc gaatgagaca aaacagttag cgcaacttta tctagatctt tagggttttc
     6061 atcacgcaat ttcagaatga tattttcctc acgaacattt ggaccattga ataacttttc
     6121 aacttcgtaa tattcttcca agaaatggac aaatatagaa tgttcatggg cttctaaccc
     6181 gttagagtac ttatgagcaa tatccgccaa tggttccacg acggcgccca gcaatttgtc
     6241 ggggttgtat tcaggattct tcacggccat atcaatcaat ttacttaatt gtctagctgg
     6301 gaaaacagca ccacgtctca aagaacgtgc aactaactct tccatttgtt catctagctt
     6361 agcaggcaat cttgaatgta aagcagagat gtgtagtttc cattctgagt aaggcagttt
     6421 tggatttctc aaaacctcta tcaattgttg caaggaagcg ttcataataa cttggttgtc
     6481 ataacccttc aaaatgtttt ccaaagtaga cactaatgac ttgaatttat aggcaggttt
     6541 ggttccttcg ataactggag aaccaaaatc tggcagcata ccttcaaatg gtagagcgtg
     6601 cttgaccttg gatggatcgt caagagtcat aatagccatg atatcacctg caacaatggt
     6661 agaaccaggt tgctttaata actggacgat accattttct tgagaaacca aaggcatttg
     6721 cattttcata acttcaattt ctgcatatgg ttggcccttg ataatgtgtt caccattttc
     6781 caccaagaat ttaaccaatt taccagggga tggagtacgc aactgggttg gatcgttttc
     6841 aacttccaac aaagtagtca tagagtcaac ggataatctt gtagcagcaa cttcttcttt
     6901 ccaatagatg gtatgcgatt taccgcctat ggcaatcaaa agaccaccat cagatagttg
     6961 acgcagtatg atatcacatt tagaaccatt gataaataat gtgtaacggt cattaccgga
     7021 tttagctacg gtgaacttgt atcttttacc ctcatggata aaatctacag ggaacatagt
     7081 ttgcagtagg tctttagata gaacttgtcc cttttgtaag gattcgatat acttgtggcg
     7141 ggcttcttca gatgctaaga aagcctttgt agcggcaccg caaatgacgg caagagttgg
     7201 atcaggcttt tcagcggtca ttttatgagt aatcaaatcg tccaaccaac cggtggtaat
     7261 agtgttatcc tcgaaatctt cagtttccaa aagtttgatc aagtattcca cagtagttct
     7321 gaaatcaccc ctaatggaca attccttcag ggcaacaacc atgtgtttcc tggaagcttg
     7381 tctattttca ccaaaagcaa aaatatggcc gaactgagag tccgaaaagg agtgaatatt
     7441 accattgtta cccacggaga agtaacccca aacattagag gaagaacgga agtttagttc
     7501 atgcaaagta ccacccgatg gcttgaatcc atcgtttgga tcttctgatg tgatacgaca
     7561 agcggtacaa tgaccctttg gaataggtct tctttgtttc ttggtggcat cttgagtttt
     7621 gaattcgaaa tcgatttctg aggcagaatg aggattcata ccatataaag ttctaatgtc
     7681 acttattcta tgcataggga tacccatagc gatttgtaat tgagctgcag gtaagttaac
     7741 accggagacc atttccgttg ttggatgctc gacttgtaat cttgggttca attctaaaaa
     7801 gtagaatttt ccatcatcat gagaatatag atactccacg gtaccggcag agacataacc
     7861 gactagtttc cccagtctga cggcagcctt ttccatctcg tgaaatgttt cagccttggc
     7921 aattgtaact ggtgcttctt cgataatttt ttgatgacgt ctctgaacgg aacagtctct
     7981 accgaacaag gaaatatttg taccgtactg atctgctagc agttgaactt ccaagtgacg
     8041 cgctctaccg gccaacttca tgatgaaaat gggggagcct ggaatttcgt tggctgcctg
     8101 gtggtataaa gcgatgaaat cttcttcacg ttcaacttgt ctgatacctt taccaccacc
     8161 accttcggat gccttaatca tgacaggaaa accaatacgc ttggcctttt gtaaaccatc
     8221 ttcaggagag gtacaacaac ccttttgata gatgtcatcg tcgacagaga ccagaccggt
     8281 tttctcgtcc acgtgaacgg tgtcaacacc ggtaccagac catggaatac atgggacttt
     8341 agcactttga gcgacaatgg tagaggagat tttatcacct aaagacctca tggcgttacc
     8401 tggaggccca ataaagatga ctttcctctt agactgggac aatttttcag gcaatagtgg
     8461 attctcggag gcgtgacccc agccagccca tacggcgtct acgtctgctc tttcggcgat
     8521 gtctacgatc aagtctacgt tagcgtagtt gttattatta gtaccacctg gcacttcaat
     8581 gtattgatcg gccatacgga tatattctgc gttggcctcc agatcttctg gggtggccat
     8641 ggcgacgaat tggacggttc tgtcatcgcc gaacgtctcg tatgcccatt ttctgacgga
     8701 tctaatttct ttcacggcgg caataccatt atttgctatc aggatcttgg atatgaccgt
     8761 gtgaccaccg tgactcttaa caaagtccct taacggggac tcctctagtt tatctactgt
     8821 attgaggcca atgaaatgac ctggaagttc tgtatgtctt tctgagtagt ttgtaatttc
     8881 gtactccatc ttctgtggag aagactcgaa taagctttct tcgctcatgg tagaaactaa
     8941 agcggccgct tgttttatat ttgttgtaaa aagtagataa ttacttcctt gatgatctgt
     9001 aaaaaagaga aaaagaaagc atctaagaac ttgaaaaact acgaattaga aaagaccaaa
     9061 tatgtatttc ttgcattgac caatttatgc aagtttatat atatgtaaat gtaagtttca
     9121 cgaggttcta ctaaactaaa ccaccccctt ggttagaaga aaagagtgtg tgagaacagg
     9181 ctgttgttgt cacacgattc ggacaattct gtttgaaaga gagagagtaa cagtacgatc
     9241 gaacgaactt tgctctggag atcacagtgg gcatcatagc atgtggtact aaaccctttc
     9301 ccgccattcc agaaccttcg attgcttgtt acaaaacctg tgagccgtcg ctaggacctt
     9361 gttgtgtgac gaaattggaa gctgcaatca ataggaagac aggaagtcga gcgtgtctgg
     9421 gttttttcag ttttgttctt tttgcaaaca aatcacgagc gacggtaatt tctttctcga
     9481 taagaggcca cgtgctttat gagggtaaca tcaattcaag aaggagggaa acacttcctt
     9541 tttctggccc tgataatagt atgagggtga agccaaaata aaggattcgc gcccaaatcg
     9601 gcatctttaa atgcaggtat gcgatagttc ctcactcttt ccttactcac gagtaattct
     9661 tgcaaatgcc tattatgcag atgttataat atctgtgcgt cttgagttga agtcaggaat
     9721 ctaaaataaa aattaaggtt aataaaaaga ggaaagaaaa aaaaattaat cgatttacag
     9781 aaacttgcac actaaaaata cacaactaaa agcaattaca gtatgggaag tcatcgacgt
     9841 tatctctact atagtatatt atcatttcta ttattatcct gctcagtggt acttgcaaaa
     9901 caagataaga ccccattctt tgaaggtact tccaggccgg ccgcacacac catagcttca
     9961 aaatgtttct actccttttt tactcttcca gattttctcg gactccgcgc atcgccgtac
    10021 cacttcaaaa cacccaagca cagcatacta aatttcccct ctttcttcct ctagggtgtc
    10081 gttaattacc cgtactaaag gtttggaaaa gaaaaaagag accgcctcgt ttctttttct
    10141 tcgtcgaaaa aggcaataaa aatttttatc acgtttcttt ttcttgaaaa tttttttttt
    10201 tgattttttt ctctttcgat gacctcccat tgatatttaa gttaataaac ggtcttcaat
    10261 ttctcaagtt tcagtttcat ttttcttgtt ctattacaac tttttttact tcttgctcat
    10321 tagaaagaaa gcatagcaat ctaatctaag ttttaattac aaggatccgt aatacgactc
    10381 actatagggc ccgggcgtcg acatggaaca gaagttgatt tccgaagaag acctcgagta
    10441 agcttggtac cgcggctagc taagatccgc tctaaccgaa aaggaaggag ttagacaacc
    10501 tgaagtctag gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat
    10561 atttcaaatt tttctttttt ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa
    10621 aaccttgctt gagaaggttt tgggacgctc gaagatcctc cggatcgttt cgccggcgtt
    10681 tatccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
    10741 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
    10801 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
    10861 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
    10921 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
    10981 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
    11041 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
    11101 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
    11161 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
    11221 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
    11281 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
    11341 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
    11401 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
    11461 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
    11521 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
    11581 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
    11641 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
    11701 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
    11761 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
    11821 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    11881 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    11941 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    12001 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    12061 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    12121 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    12181 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    12241 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    12301 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    12361 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    12421 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    12481 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    12541 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    12601 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    12661 aaaagtgcca cctgaacgaa gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga
    12721 gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg
    12781 cgaaagcgct attttaccaa cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa
    12841 cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg
    12901 caacgcgaga gcgctatttt accaacaaag aatctatact tcttttttgt tctacaaaaa
    12961 tgcatcccga gagcgctatt tttctaacaa agcatcttag attacttttt ttctcctttg
    13021 tgcgctctat aatgcagtct cttgataact ttttgcactg taggtccgtt aaggttagaa
    13081 gaaggctact ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc acttcccgcg
    13141 tttactgatt actagcgaag ctgcgggtgc attttttcaa gataaaggca tccccgatta
    13201 tattctatac cgatgtggat tgcgcatact ttgtgaacag aaagtgatag cgttgatgat
    13261 tcttcattgg tcagaaaatt atgaacggtt tcttctattt tgtctctata tactacgtat
    13321 aggaaatgtt tacattttcg tattgttttc gattcactct atgaatagtt cttactacaa
    13381 tttttttgtc taaagagtaa tactagagat aaacataaaa aatgtagagg tcgagtttag
    13441 atgcaagttc aaggagcgaa aggtggatgg gtaggttata tagggatata gcacagagat
    13501 atatagcaaa gagatacttt tgagcaatgt ttgtggaagc ggtattcgca atattttagt
    13561 agctcgttac agtccggtgc gtttttggtt ttttgaaagt gcgtcttcag agcgcttttg
    13621 gttttcaaaa gcgctctgaa gttcctatac tttctagaga ataggaactt cggaatagga
    13681 acttcaaagc gtttccgaaa acgagcgctt ccgaaaatgc aacgcgagct gcgcacatac
    13741 agctcactgt tcacgtcgca cctatatctg cgtgttgcct gtatatatat atacatgaga
    13801 agaacggcat agtgcgtgtt tatgcttaaa tgcgtactta tatgcgtcta tttatgtagg
    13861 atgaaaggta gtctagtacc tcctgtgata ttatcccatt ccatgcgggg tatcgtatgc
    13921 ttccttcagc actacccttt agctgttcta tatgctgcca ctcctcaatt ggattagtct
    13981 catccttcaa tgctatcatt tcctttgata ttggatcata ctaagaaacc attattatca
    14041 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc
//
