LOCUS       sequence-113022-       14862 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast expression plasmid: acsL641P, ALD6, ADH2, ERG10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14862)
  AUTHORS   Chen Y, Daviet L, Schalk M, Siewers V, Nielsen J
  TITLE     Establishing a platform cell factory through engineering of yeast 
            acetyl-CoA metabolism.
  JOURNAL   Metab Eng. 2013 Jan;15:48-54. doi: 10.1016/j.ymben.2012.11.002. Epub
            2012 Nov 17.
  PUBMED    23164578
REFERENCE   2  (bases 1 to 14862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2013-01"; volume: "15:48-54. doi"; pages: " 
            10.1016/j.ymben.2012.11.002. Epub 2012 Nov 17"
COMMENT     Sequence Label: pIYC08
FEATURES             Location/Qualifiers
     source          1..14862
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        317..503
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             504..1163
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFL
                     ESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     rep_origin      complement(1423..1878)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1560..1581)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1772..1791
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(2104..2403)
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     terminator      complement(4062..4227)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     promoter        complement(6350..6756)
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     terminator      9298..9487
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        9499..9905
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     terminator      11278..11443
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(11531..11548)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11702..12290)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11782..11801)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12461..13252,13253..13321))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13084..13103
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13322..13426)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      13453..14795
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(14416..14463)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     14835..14853
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaatt cccgttttaa gagcttggtg agcgctagga gtcactgcca ggtatcgttt
      241 gaacacggca ttagtcaggg aagtcataac acagtccttt cccgcaattt tctttttcta
      301 ttactcttgg cctcctctag tacactctat atttttttat gcctcggtaa tgattttcat
      361 tttttttttt cccctagcgg atgactcttt ttttttctta gcgattggca ttatcacata
      421 atgaattata cattatataa agtaatgtga tttcttcgaa gaatatacta aaaaatgagc
      481 aggcaagata aacgaaggca aagatgacag agcagaaagc cctagtaaag cgtattacaa
      541 atgaaaccaa gattcagatt gcgatctctt taaagggtgg tcccctagcg atagagcact
      601 cgatcttccc agaaaaagag gcagaagcag tagcagaaca ggccacacaa tcgcaagtga
      661 ttaacgtcca cacaggtata gggtttctgg accatatgat acatgctctg gccaagcatt
      721 ccggctggtc gctaatcgtt gagtgcattg gtgacttaca catagacgac catcacacca
      781 ctgaagactg cgggattgct ctcggtcaag cttttaaaga ggccctactg gcgcgtggag
      841 taaaaaggtt tggatcagga tttgcgcctt tggatgaggc actttccaga gcggtggtag
      901 atctttcgaa caggccgtac gcagttgtcg aacttggttt gcaaagggag aaagtaggag
      961 atctctcttg cgagatgatc ccgcattttc ttgaaagctt tgcagaggct agcagaatta
     1021 ccctccacgt tgattgtctg cgaggcaaga atgatcatca ccgtagtgag agtgcgttca
     1081 aggctcttgc ggttgccata agagaagcca cctcgcccaa tggtaccaac gatgttccct
     1141 ccaccaaagg tgttcttatg tagtgacacc gattatttaa agctgcagca tacgatatat
     1201 atacatgtgt atatatgtat acctatgaat gtcagtaagt atgtatacga acagtatgat
     1261 actgaagatg acaaggtaat gcatcattct atacgtgtca ttctgaacga ggcgcgcttt
     1321 ccttttttct ttttgctttt tctttttttt tctcttgaac tcgacggatc tatgcggtgt
     1381 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata
     1441 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     1501 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     1561 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     1621 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     1681 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     1741 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     1801 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     1861 cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
     1921 gatcggtgcg ggcctcttcg ctattacgcc agctggataa aggcgcgcca cgtcaagacg
     1981 aaaagtgaaa aattacatcc gtatagaatt atataacttg atgagatgag atgagtaaat
     2041 gacagaagaa ttaccgtttc atcattgaac ttcgatcatt tcaatgctgg catgcgaagg
     2101 aaaatgagaa atatcgaggg agacgattca gaggagcagg acaaactata accgactgtt
     2161 tgttggagga tgccgtacat aacgaacact gctgaagcta ccatgtctac agtttagagg
     2221 aatgggtaca actcacaggc gagggatggt gttcactcgt gctagcaaac gcggtgggag
     2281 caaaaagtag aatattatct tttattcgtg aaacttcgaa cactgtcatc taaagatgct
     2341 atatactaat ataggcatac ttgataatga aaactataaa tcgtaaagac ataagagatc
     2401 cgcttattta gaagtgtcaa caacgtatct accagcaatt tggcccttct ccatcttttc
     2461 gtaaatttct ggtaaactgg ataagccaac tacctttatt ggagacttga ctagacctct
     2521 ggcaaagaaa tctaaggctt ctctggtatc agctctgttc cccacgtaag agccgacaat
     2581 ggagatagac ttgacaacgt ggttgaagac atcagaggag cactttgcac cggctggcaa
     2641 accaaccaag acaacagtac cgttcgccct acagtatctg gtagaagctt cgatagcggc
     2701 ttcggaaacg gaaacattga tgataccgtg ggcaccgccg ttggtagcct taacgactgc
     2761 gctaacaatg tccttctctt tggtgaagtc gatgaatact tcaccaccga gcgaggtaaa
     2821 caattcttcc tttcctggac caccatcaat acctaagact ctgtaaccca tcgccttagc
     2881 atattgaaca gccaaagaac ctagaccacc agcagcacca gaaatggccg cccagtggcc
     2941 tgctctcaag ttggcagact tcaaagcctt gtatacggtg ataccagcac acaagattgg
     3001 cgcgacttca gccaagtcag taccttgagg aatgtgagcg gcttgaacag cgtcagcggt
     3061 agcgtattct tggaaagaac cgtcgtgggt gtaaccagac aagtcagcgt gaggacagtt
     3121 ggattcgtta cccaattcac agtattcaca ggccatacaa gaaccgttca accatttgat
     3181 accggcgtag tcaccgatct tccagccctt aacgttttca cccatgccga caacgacacc
     3241 ggcaccttcg tgaccaccaa ctaatggtaa cttagttggc aatggccagt caccatgcca
     3301 agcgtgcaaa tcggtgtggc agacaccaga gtacttgacg ttgattaaca attcgttggg
     3361 ctttggcttt ggaactggga tatccttatg ctccaacttg ccgttggatt cgtagaagat
     3421 aatggctttt tgagtttctg gaatagacat tgttttggat cctttttgat taaaattaaa
     3481 aaaacttttt gtttttgtgt ttattctttg ttcttagaaa agacaagttg agcttgtttg
     3541 ttcttgatgt tttattattt tacaatagct gcaaatgaag aatagattcg aacattgtga
     3601 agtattggca tatatcgtct ctatttatac tttttttttt tcagttctag tatattttgt
     3661 attttcctcc ttttcattct ttcagttgcc aataagttac aggggatctc gaaagatggt
     3721 ggggattttt ccttgaaaga cgactttttg ccatctaatt tttccttgtt gcctctgaaa
     3781 attatccagc agaagcaaat gtaaaagatg aacctcagaa gaacacgcag gggcccgaaa
     3841 ttgttcctac gagaagtagt gggtcataaa aagtttattc cctggaaaaa aaattttgcg
     3901 ttgcctttct ggagaatttt ttcgaattag cgtgctgcca ctgcatgcat ttctgagaag
     3961 tgtgggcatt cttccaccag ttgttcctcc taaaaaaaaa aagatttcct accccgcaca
     4021 aattcctgca tacccctcat ttccacgggg cgcgccaatt ggagcgacct catgctatac
     4081 ctgagaaagc aacctgacct acaggaaaga gttactcaag aataagaatt ttcgttttaa
     4141 aacctaagag tcactttaaa atttgtatac acttattttt tttataactt atttaataat
     4201 aaaaatcata aatcataaga aattcgctta tttagaagtg tcaacaacgt atctaccaac
     4261 gatttgaccc ttttccatct tttcgtaaat ttctggcaag gtagacaagc cgacaacctt
     4321 gattggagac ttgaccaaac ctctggcgaa gaattgttaa ttaatcatga tggcatagca
     4381 atagcttgtt tttcctctaa aggtttttcg accacgcctg gatcggcaag agtagaagta
     4441 tcacctaagt tagacgtatc tccggcggcg atctttctaa gtatacgtct cataatcttt
     4501 ccagatcttg tcttaggcaa tgaatctgtc caatgcaaaa catctggtgt ggctaaaggc
     4561 cctatttcct ttcgaaccca atttctgacc tctgcgtata gctctggaga tggttcctca
     4621 ccatggttga gtgtaacata tgcgtatatg gcttgaccct tgatagcgtg tgggatgcca
     4681 acgactgcag cttcagcaat cttaggatga gcaactaaag ctgactcaat ctcagctgta
     4741 cccagacgat gtccagatac gttaaggacg tcatctactc tgccggtaat ccagtagtac
     4801 ccatcttcgt ctcttctcgc accgtcaccg ctgaaataca tgtttttgaa agttgagaaa
     4861 taggtttgtt cgaatctttc gtgatcacca aacaatgttc tagcctgacc aggccaggaa
     4921 tcagttatca ctagattgcc ttctgttgcc ccctcttgtg gatgcccttc gttgtctact
     4981 aaagcaggtt ggacgccgaa gaaaggccta gtagcagacc cagccttcaa ttctattgca
     5041 cctggaagag gtgtaatcat gaatccacct gtttcagttt gccaccatgt atccactact
     5101 ggacactttt ccttaccaat ctttttccaa taccattccc aagcttctgg attgattggc
     5161 tcgccaacgg atcctaagat acgtaggcta cttctatctg ttccctcaat cgccttatct
     5221 ccttcggcca ttagcgctct aatggcggtt ggggctgtgt acaatatgtt cacttggtgt
     5281 ttatcgacca cttgacacat cctggctggg gttggccagt ttggtactcc ttcaaacatc
     5341 aaagtggttg cgccgcatgc cagtggaccg taaagcaggt aactatgacc agtaacccaa
     5401 ccgacatcag cggtacacca gtagatatca cctggatggt aatcaaaaac gtacttaaag
     5461 gttgttgcgg cgtatacgag ataaccacct gttgtatgca aaacaccttt tggtttgcca
     5521 gttgacccag atgtatacaa gatgaacaat ggatcttccg cattcattgc ttctggttgg
     5581 tgttctggac ttgctttctc aataagatct ctccaccaca aatccctacc ttcctgccag
     5641 tctatatcag agccggtacg ctttagtaca attacatgct ccacactcgt aacgtttggg
     5701 tttttcaacg cgtcatcaac gtttttcttc aatgggatgg atctacctgc tctaacacct
     5761 tcgtcggcag tgataactaa tctacttgat gagtcaatga ttctcccagc aacggcttca
     5821 ggggagaatc cgccaaagat aacgctatgg actgctccta ttctggcaca ggccaacatt
     5881 gccacagctg cctctgggac cataggcatg tagattgcca caacatcgcc ctttttgata
     5941 cccaggtcca gtaaagtatt cgcgaatctg caaacatctc tgtgtaactc tctgtaggag
     6001 atgtgcttag attgagaagt atcatcacct tcccaaatga ttgccgttct gtcaccattt
     6061 tcctggaggt gtcgatccaa gcaattagca gctaggttta gagtgccgtc ctcgtaccac
     6121 ttgattgaca catttccagg agcaaaggat gtgtttttga ccttttgata tggagtaatc
     6181 caatcgagta tcttaccttg ctcgccccag aaagtatcag gatcgttgat agactgcttg
     6241 tacttcgttt cgtattgttc agggttgatt aagcacctgt cagcgatatt cgcaggaata
     6301 gcatgtttgt gtgtttgtga cattgttttg cggccgcttg taattaaaac ttagattaga
     6361 ttgctatgct ttctttctaa tgagcaagaa gtaaaaaaag ttgtaataga acaagaaaaa
     6421 tgaaactgaa acttgagaaa ttgaagaccg tttattaact taaatatcaa tgggaggtca
     6481 tcgaaagaga aaaaaatcaa aaaaaaaaat tttcaagaaa aagaaacgtg ataaaaattt
     6541 ttattgcctt tttcgacgaa gaaaaagaaa cgaggcggtc tcttttttct tttccaaacc
     6601 tttagtacgg gtaattaacg acaccctaga ggaagaaaga ggggaaattt agtatgctgt
     6661 gcttgggtgt tttgaagtgg tacggcgatg cgcggagtcc gagaaaatct ggaagagtaa
     6721 aaaaggagta gaaacatttt gaagctatgg tgtgtgcggc cggcctggaa gtaccttcaa
     6781 agaatggggt cttatcttgt tttgcaagta ccactgagca ggataataat agaaatgata
     6841 atatactata gtagagataa cgtcgatgac ttcccatact gtaattgctt ttagttgtgt
     6901 atttttagtg tgcaagtttc tgtaaatcga ttaatttttt tttctttcct ctttttatta
     6961 accttaattt ttattttaga ttcctgactt caactcaaga cgcacagata ttataacatc
     7021 tgcataatag gcatttgcaa gaattactcg tgagtaagga aagagtgagg aactatcgca
     7081 tacctgcatt taaagatgcc gatttgggcg cgaatccttt attttggctt caccctcata
     7141 ctattatcag ggccagaaaa aggaagtgtt tccctccttc ttgaattgat gttaccctca
     7201 taaagcacgt ggcctcttat cgagaaagaa attaccgtcg ctcgtgattt gtttgcaaaa
     7261 agaacaaaac tgaaaaaacc cagacacgct cgacttcctg tcttcctatt gattgcagct
     7321 tccaatttcg tcacacaaca aggtcctagc gacggctcac aggttttgta acaagcaatc
     7381 gaaggttctg gaatggcggg aaagggttta gtaccacatg ctatgatgcc cactgtgatc
     7441 tccagagcaa agttcgttcg atcgtactgt tactctctct ctttcaaaca gaattgtccg
     7501 aatcgtgtga caacaacagc ctgttctcac acactctttt cttctaacca agggggtggt
     7561 ttagtttagt agaacctcgt gaaacttaca tttacatata tataaacttg cataaattgg
     7621 tcaatgcaag aaatacatat ttggtctttt ctaattcgta gtttttcaag ttcttagatg
     7681 ctttcttttt ctctttttta cagatcatca aggaagtaat tatctacttt ttacaacaaa
     7741 tataaaacaa ggatccaaaa caatgactaa gctacacttt gacactgctg aaccagtcaa
     7801 gatcacactt ccaaatggtt tgacatacga gcaaccaacc ggtctattca ttaacaacaa
     7861 gtttatgaaa gctcaagacg gtaagaccta tcccgtcgaa gatccttcca ctgaaaacac
     7921 cgtttgtgag gtctcttctg ccaccactga agatgttgaa tatgctatcg aatgtgccga
     7981 ccgtgctttc cacgacactg aatgggctac ccaagaccca agagaaagag gccgtctact
     8041 aagtaagttg gctgacgaat tggaaagcca aattgacttg gtttcttcca ttgaagcttt
     8101 ggacaatggt aaaactttgg ccttagcccg tggggatgtt accattgcaa tcaactgtct
     8161 aagagatgct gctgcctatg ccgacaaagt caacggtaga acaatcaaca ccggtgacgg
     8221 ctacatgaac ttcaccacct tagagccaat cggtgtctgt ggtcaaatta ttccatggaa
     8281 ctttccaata atgatgttgg cttggaagat cgccccagca ttggccatgg gtaacgtctg
     8341 tatcttgaaa cccgctgctg tcacaccttt aaatgcccta tactttgctt ctttatgtaa
     8401 gaaggttggt attccagctg gtgtcgtcaa catcgttcca ggtcctggta gaactgttgg
     8461 tgctgctttg accaacgacc caagaatcag aaagctggct tttaccggtt ctacagaagt
     8521 cggtaagagt gttgctgtcg actcttctga atctaacttg aagaaaatca ctttggaact
     8581 aggtggtaag tccgcccatt tggtctttga cgatgctaac attaagaaga ctttaccaaa
     8641 tctagtaaac ggtattttca agaacgctgg tcaaatttgt tcctctggtt ctagaattta
     8701 cgttcaagaa ggtatttacg acgaactatt ggctgctttc aaggcttact tggaaaccga
     8761 aatcaaagtt ggtaatccat ttgacaaggc taacttccaa ggtgctatca ctaaccgtca
     8821 acaattcgac acaattatga actacatcga tatcggtaag aaagaaggcg ccaagatctt
     8881 aactggtggc gaaaaagttg gtgacaaggg ttacttcatc agaccaaccg ttttctacga
     8941 tgttaatgaa gacatgagaa ttgttaagga agaaattttt ggaccagttg tcactgtcgc
     9001 aaagttcaag actttagaag aaggtgtcga aatggctaac agctctgaat tcggtctagg
     9061 ttctggtatc gaaacagaat ctttgagcac aggtttgaag gtggccaaga tgttgaaggc
     9121 cggtaccgtc tggatcaaca catacaacga ttttgactcc agagttccat tcggtggtgt
     9181 taagcaatct ggttacggta gagaaatggg tgaagaagtc taccatgcat acactgaagt
     9241 aaaagctgtc agaattaagt tgtaactcga gtaagcttgg taccgcggct agctaagatc
     9301 cgctctaacc gaaaaggaag gagttagaca acctgaagtc taggtcccta tttatttttt
     9361 tatagttatg ttagtattaa gaacgttatt tatatttcaa atttttcttt tttttctgta
     9421 cagacgcgtg tacgcatgta acattatact gaaaaccttg cttgagaagg ttttgggacg
     9481 ctcgaagatc ctccggagca cacaccatag cttcaaaatg tttctactcc ttttttactc
     9541 ttccagattt tctcggactc cgcgcatcgc cgtaccactt caaaacaccc aagcacagca
     9601 tactaaattt cccctctttc ttcctctagg gtgtcgttaa ttacccgtac taaaggtttg
     9661 gaaaagaaaa aagagaccgc ctcgtttctt tttcttcgtc gaaaaaggca ataaaaattt
     9721 ttatcacgtt tctttttctt gaaaattttt ttttttgatt tttttctctt tcgatgacct
     9781 cccattgata tttaagttaa taaacggtct tcaatttctc aagtttcagt ttcatttttc
     9841 ttgttctatt acaacttttt ttacttcttg ctcattagaa agaaagcata gcaatctaat
     9901 ctaagtttta attacaagcg gccgcactag taaaacaatg tctcagaacg tttacattgt
     9961 atcgactgcc agaaccccaa ttggttcatt ccagggttct ctatcctcca agacagcagt
    10021 ggaattgggt gctgttgctt taaaaggcgc cttggctaag gttccagaat tggatgcatc
    10081 caaggatttt gacgaaatta tttttggtaa cgttctttct gccaatttgg gccaagctcc
    10141 ggccagacaa gttgctttgg ctgccggttt gagtaatcat atcgttgcaa gcacagttaa
    10201 caaggtctgt gcatccgcta tgaaggcaat cattttgggt gctcaatcca tcaaatgtgg
    10261 taatgctgat gttgtcgtag ctggtggttg tgaatctatg actaacgcac catactacat
    10321 gccagcagcc cgtgcgggtg ccaaatttgg ccaaactgtt cttgttgatg gtgtcgaaag
    10381 agatgggttg aacgatgcgt acgatggtct agccatgggt gtacacgcag aaaagtgtgc
    10441 ccgtgattgg gatattacta gagaacaaca agacaatttt gccatcgaat cctaccaaaa
    10501 atctcaaaaa tctcaaaagg aaggtaaatt cgacaatgaa attgtacctg ttaccattaa
    10561 gggatttaga ggtaagcctg atactcaagt cacgaaggac gaggaacctg ctagattaca
    10621 cgttgaaaaa ttgagatctg caaggactgt tttccaaaaa gaaaacggta ctgttactgc
    10681 cgctaacgct tctccaatca acgatggtgc tgcagccgtc atcttggttt ccgaaaaagt
    10741 tttgaaggaa aagaatttga agcctttggc tattatcaaa ggttggggtg aggccgctca
    10801 tcaaccagct gattttacat gggctccatc tcttgcagtt ccaaaggctt tgaaacatgc
    10861 tggcatcgaa gacatcaatt ctgttgatta ctttgaattc aatgaagcct tttcggttgt
    10921 cggtttggtg aacactaaga ttttgaagct agacccatct aaggttaatg tatatggtgg
    10981 tgctgttgct ctaggtcacc cattgggttg ttctggtgct agagtggttg ttacactgct
    11041 atccatctta cagcaagaag gaggtaagat cggtgttgcc gccatttgta atggtggtgg
    11101 tggtgcttcc tctattgtca ttgaaaagat atgagagctc ttaattaaca attcttcgcc
    11161 agaggtttgg tcaagtctcc aatcaaggtt gtcggcttgt ctaccttgcc agaaatttac
    11221 gaaaagatgg aaaagggtca aatcgttggt agatacgttg ttgacacttc taaataagcg
    11281 aatttcttat gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac
    11341 aaattttaaa gtgactctta ggttttaaaa cgaaaattct tattcttgag taactctttc
    11401 ctgtaggtca ggttgctttc tcaggtatag catgaggtcg ctccaattcg ccggcgttta
    11461 tccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
    11521 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
    11581 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
    11641 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
    11701 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
    11761 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
    11821 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
    11881 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
    11941 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
    12001 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
    12061 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    12121 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
    12181 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
    12241 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
    12301 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    12361 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
    12421 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
    12481 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
    12541 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
    12601 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
    12661 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
    12721 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    12781 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    12841 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    12901 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    12961 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    13021 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    13081 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    13141 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    13201 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    13261 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    13321 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    13381 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    13441 aagtgccacc tgaacgaagc atctgtgctt cattttgtag aacaaaaatg caacgcgaga
    13501 gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg
    13561 aaagcgctat tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa aaatgcaacg
    13621 cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca
    13681 acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc tacaaaaatg
    13741 catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt ctcctttgtg
    13801 cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa ggttagaaga
    13861 aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac ttcccgcgtt
    13921 tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc cccgattata
    13981 ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg ttgatgattc
    14041 ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata ctacgtatag
    14101 gaaatgttta cattttcgta ttgttttcga ttcactctat gaatagttct tactacaatt
    14161 tttttgtcta aagagtaata ctagagataa acataaaaaa tgtagaggtc gagtttagat
    14221 gcaagttcaa ggagcgaaag gtggatgggt aggttatata gggatatagc acagagatat
    14281 atagcaaaga gatacttttg agcaatgttt gtggaagcgg tattcgcaat attttagtag
    14341 ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag cgcttttggt
    14401 tttcaaaagc gctctgaagt tcctatactt tctagagaat aggaacttcg gaataggaac
    14461 ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag
    14521 ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt atatatatat acatgagaag
    14581 aacggcatag tgcgtgttta tgcttaaatg cgtacttata tgcgtctatt tatgtaggat
    14641 gaaaggtagt ctagtacctc ctgtgatatt atcccattcc atgcggggta tcgtatgctt
    14701 ccttcagcac taccctttag ctgttctata tgctgccact cctcaattgg attagtctca
    14761 tccttcaatg ctatcatttc ctttgatatt ggatcatcta agaaaccatt attatcatga
    14821 cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//
