LOCUS       sequence-113026-         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Wang NS, Unkila MT, Reineks EZ, Distelhorst CW
  TITLE     Transient expression of wild-type or mitochondrially targeted Bcl-2 
            induces apoptosis, whereas transient expression of endoplasmic 
            reticulum-targeted Bcl-2 is protective against Bax-induced cell 
            death.
  JOURNAL   J Biol Chem. 2001 Nov 23. 276(47):44117-28.
  PUBMED    11546793
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2001 Nov 23. 276(47):44117-28."
COMMENT     Sequence Label: Flag-Bcl2
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     18..34
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     19..35
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(791..814)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      814..823
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(858..876)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aggtaccggg ccccccctcg aggtcgacgg tatcgataag cttgatatcg aattctcact
       61 tgtggctcag ataggcaccc agggtgatgc aagctcccac cagggccaaa ctgagcagag
      121 tcttcagaga cagccaggag aaatcaaaca gaggccgcat gctggggccg tacagttcca
      181 caaaggcatc ccagcctccg ttatcctgga tccaggtgtg caggtgccgg ttcaggtact
      241 cagtcatcca cagggcgatg ttgtccacca ggggcgacat ctcccggttg acgctctcca
      301 cacacatgac cccaccgaac tcaaagaagg ccacaatcct cccccagttc accccgtccc
      361 tgaagagctc ctccaccacc gtggcaaagc gtccccgcgc ggtgaagggc gtcaggtgca
      421 gctggctgga catctcggcg aagtcgcggc ggtagcggcg ggagaagtcg tcgccggcct
      481 ggcggagggt caggtggacc acaggtggca ccgggctgag cgcaggcccc gcggcggcgc
      541 cgggggcagc cggggtctgc agcggcgagg tcctggcgac cgggtcgcgg gatgcggctg
      601 gatggggcgt gtgcccgggc tgggaggaga agatgcccgg tgcgggggcg gcccccgggg
      661 gcgcggcgcc cacatctccc gcatcccact cgtagcccct ctgcgacagc ttataatgga
      721 tgtacttcat cactatctcc cggttatcgt accccgttct cccagcgtgc gccatagatc
      781 cgcccgggct cttatcgtcg tcatccttgt aatccatggt ggcggccgcc accgcggtgg
      841 agctccagct tttgttccct ttagtgaggg ttaatttcga gcttggcgta atcgctagcg
      901 gatctgacgg ttcactaaac cagctctgct tatata
//
