LOCUS       sequence-113027-         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombination-Induced Tag Exchange (RITE) Cassette, 3x-HA-GFP to a 
            3xT7-RFP tag, Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Menendez-Benito V, van Deventer SJ, Jimenez-Garcia V, Roy-Luzarraga 
            M, van Leeuwen F, Neefjes J
  TITLE     Spatiotemporal analysis of organelle and macromolecular complex 
            inheritance.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Jan 2;110(1):175-80. doi: 
            10.1073/pnas.1207424110. Epub 2012 Dec 17.
  PUBMED    23248297
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1207424110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-01-2- 2"; volume: "110"; issue: "1"; pages: "175-80"
COMMENT     Sequence Label: pKV016
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      147..344
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     terminator      complement(463..650)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     571..595
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
       61 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
      121 cgtccgaggg caaaggaata atcagtactg acaataaaaa gattcttgtt ttcaagaact
      181 tgtcatttgt atagtttttt tatattgtag ttgttctatt ttaatcaaat gttagcgtga
      241 tttatatttt ttttcgcctc gacatcatct gcccagatgc gaagttaagt gcgcagaaag
      301 taatatcatg cgtcaatcgt atgtgaatgc tggtcgctat actgctgtcg attcgatact
      361 aacgccgcca tccagtgtcg aaaacgagct cgaattcatc gatgattatg gtgcactctc
      421 agtacaatct ggatccttac cctgttatcc ctagcggatc tgccggtaga ggtgtggtca
      481 ataagagcga cctcatacta tacctgagaa agcaacctga cctacaggaa agagttactc
      541 aagaataaga attttcgttt taaaacctaa gagtcacttt aaaatttgta tacacttatt
      601 ttttttataa cttatttaat aataaaaatc ataaatcata agaaattcgc ttatttagaa
      661 gttaactagt tttatacaat tcatccatac catgggtaat accagcagca gtaacaaatt
      721 ctaacaagac catgtggtct ctcttttcgt ttggatcttt ggataaggca gattgagtgg
      781 ataagtaatg gttgtctggt aacaagactg gaccatcacc aattggagta ttttgttgat
      841 aatggtcagc taattgaaca gaaccatctt caatgttgtg tctaattttg aagttaactt
      901 tgataccatt cttttgtttg tcagccatga tgtaaacatt gtgagagtta tagttgtatt
      961 ccaatttgtg acctaaaatg ttacca
//
