LOCUS       sequence-113028-        1027 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombination-Induced Tag Exchange (RITE) Cassette, 3x-HA-GFP to a 
            3xT7-RFP tag, Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Menendez-Benito V, van Deventer SJ, Jimenez-Garcia V, Roy-Luzarraga 
            M, van Leeuwen F, Neefjes J
  TITLE     Spatiotemporal analysis of organelle and macromolecular complex 
            inheritance.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Jan 2;110(1):175-80. doi: 
            10.1073/pnas.1207424110. Epub 2012 Dec 17.
  PUBMED    23248297
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1207424110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-01-2- 2"; volume: "110"; issue: "1"; pages: "175-80"
COMMENT     Sequence Label: pKV016
FEATURES             Location/Qualifiers
     source          1..1027
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(100..132)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     protein_bind    complement(141..174)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        269..612
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 gtcctcggag gaggccattg tacatcccat ttgctggcca ccggtcattg aggccattcc
       61 tcccatctgt tgacctcctg tcatagaagc catagtacaa cccatttgct gtccaccagt
      121 catgcttgcc atgtcgactg ataacttcgt atagcataca ttatacgaag ttattctaga
      181 ctgtgcggta tttcacaccg catagggtta attaaggcgc gccagatctg tttagcttgc
      241 ctcgtccccg ccgggtcacc cggccagcga catggaggcc cagaataccc tccttgacag
      301 tcttgacgtg cgcagctcag gggcatgatg tgactgtcgc ccgtacattt agcccataca
      361 tccccatgta taatcatttg catccataca ttttgatggc cgcacggcgc gaagcaaaaa
      421 ttacggctcc tcgctgcgga cctgcgagca gggaaacgct cccctcacag acgcgttgaa
      481 ttgtccccac gccgcgcccc tgtagagaaa tataaaaggt taggatttgc cactgaggtt
      541 cttctttcat atacttcctt ttaaaatctt gctaggatac agttctcaca tcacatccga
      601 acataaacaa ccatgggtaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg
      661 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
      721 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
      781 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
      841 gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca
      901 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc
      961 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
     1021 ttcggac
//
