LOCUS       sequence-11321-e       11132 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11132)
  AUTHORS   Auer S, Sturzebecher AS, Juttner R, Santos-Torres J, Hanack C, Frahm
            S, Liehl B, Ibanez-Tallon I
  TITLE     Silencing neurotransmission with membrane-tethered toxins.
  JOURNAL   Nat Methods. 2010 Mar;7(3):229-36. doi: 10.1038/nmeth.1425. Epub 
            2010 Feb 7.
  PUBMED    20139968
REFERENCE   2  (bases 1 to 11132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1425"; journalName: "Nat Methods"; date: "2010-03"; 
            volume: "7"; issue: "3"; pages: "229-36"
COMMENT     Sequence Label: pFU-cMVIIA-PE
FEATURES             Location/Qualifiers
     source          1..11132
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2616..3827
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3664..3683
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(3874..3907)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             3921..4598
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNKDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(4101..4121)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     protein_bind    complement(4628..4661)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4676..4720
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /translation="MSALLILALVGAAVA"
     CDS             4799..4822
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4895..5041
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     regulatory      5060..5069
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5066..5068,5069..5071,5072..5785)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5066..5068,5069..5071,5072..5782)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5111..5132)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5372..5391)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5719..5740
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5824..6412
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5877..5897)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6295..6306)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6415..6431)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6416..6432)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6937..7117
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7143..7160)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7149..7373
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7419..7847
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7506..7525)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7716..7737
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7856..7876)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7861..8190
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8041..8176
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8103..8122
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8238..8285
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8304..8678
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8808..8929
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8845..8864)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8899..8918
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8978..8994)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8978..8994)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8991..9013)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9002..9018
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9026..9032,9033..9050,9051..9056))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9071..9092
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9209..9226)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9380..9968)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9460..9479)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10139..10930,10931..10999))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10762..10781
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11000..11104)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta agggtgcagc gatctggcct ccgcgccggg ttttggcgcc
     2641 tcccgcgggc gcccccctcc tcacggcgag cgctgccacg tcagacgaag ggcgcagcga
     2701 gcgtcctgat ccttccgccc ggacgctcag gacagcggcc cgctgctcat aagactcggc
     2761 cttagaaccc cagtatcagc agaaggacat tttaggacgg gacttgggtg actctagggc
     2821 actggttttc tttccagaga gcggaacagg cgaggaaaag tagtcccttc tcggcgattc
     2881 tgcggaggga tctccgtggg gcggtgaacg ccgatgatta tataaggacg cgccgggtgt
     2941 ggcacagcta gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg
     3001 tgatcgtcac ttggtgagta gcgggctgct gggctggccg gggctttcgt ggccgccggg
     3061 ccgctcggtg ggacggaagc gtgtggagag accgccaagg gctgtagtct gggtccgcga
     3121 gcaaggttgc cctgaactgg gggttggggg gagcgcagca aaatggcggc tgttcccgag
     3181 tcttgaatgg aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca
     3241 tggtgggcgg caagaaccca aggtcttgag gccttcgcta atgcgggaaa gctcttattc
     3301 gggtgagatg ggctggggca ccatctgggg accctgacgt gaagtttgtc actgactgga
     3361 gaactcgggt ttgtcgtctg gttgcggggg cggcagttat gcggtgccgt tgggcagtgc
     3421 acccgtacct ttgggagcgc gcgcctcgtc gtgtcgtgac gtcacccgtt ctgttggctt
     3481 ataatgcagg gtggggccac ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga
     3541 cgcagggttc gggcctaggg taggctctcc tgaatcgaca ggcgccggac ctctggtgag
     3601 gggagggata agtgaggcgt cagtttcttt ggtcggtttt atgtacctat cttcttaagt
     3661 agctgaagct ccggttttga actatgcgct cggggttggc gagtgtgttt tgtgaagttt
     3721 tttaggcacc ttttgaaatg taatcatttg ggtcaatatg taattttcag tgttagacta
     3781 gtaaattgtc cgctaaattc tggccgtttt tggctttttt gttagacgaa gcttgggctg
     3841 caggtcgact ctagaggatc cccgggtacc ggtataactt cgtatagcat acattatacg
     3901 aagttattct agacacaacc atggcctcct ccgagaacgt catcaccgag ttcatgcgct
     3961 tcaaggtgcg catggagggc accgtgaacg gccacgagtt cgagatcgag ggcgagggcg
     4021 agggccgccc ctacgagggc cacaacaccg tgaagctgaa ggtgaccaag ggcggccccc
     4081 tgcccttcgc ctgggacatc ctgtcccccc agttccagta cggctccaag gtgtacgtga
     4141 agcaccccgc cgacatcccc gactacaaga agctgtcctt ccccgagggc ttcaagtggg
     4201 agcgcgtgat gaacttcgag gacggcggcg tggcgaccgt gacccaggac tcctccctgc
     4261 aggacggctg cttcatctac aaggtgaagt tcatcggcgt gaacttcccc tccgacggcc
     4321 ccgtgatgca gaagaagacc atgggctggg aggcctccac cgagcgcctg tacccccgcg
     4381 acggcgtgct gaagggcgag acccacaagg ccctgaagct gaaggacggc ggccactacc
     4441 tggtggagtt caagtctatc tacatggcca agaagcccgt gcagctgccc ggctactact
     4501 acgtggacgc caagctggac atcacctccc acaacaagga ctacaccatc gtggagcagt
     4561 acgagcgcac cgagggccgc caccacctgt tcctgtagag ctcgcgactc tagactgagt
     4621 gagtgacata acttcgtata gcatacatta tacgaagtta tataccggtg ccaccatgtc
     4681 tgcacttctg atcctagctc ttgttggagc tgcagttgct tgcaaaggca agggcgcgaa
     4741 gtgctcccgc ctcatgtatg actgttgcac cggatcgtgt aggtccggta agtgcatcga
     4801 ttacaaagac gatgacgaca agcttgcggc cgctggtaac ggaaatggca acgggaatgg
     4861 taacggaaac ggcaacgggg atggtactcg agttgctgtg ggccaggaca cgcaggaggt
     4921 catcgtggtg ccacactcct tgccctttaa ggtggtggtg atctcagcca tcctggccct
     4981 ggtggtgctc accatcatct cccttatcat cctcatcatg ctttggcaga agaaaccacg
     5041 taggatccac cggccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
     5101 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     5161 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     5221 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     5281 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     5341 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     5401 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     5461 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     5521 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     5581 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     5641 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     5701 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     5761 cggcatggac gagctgtaca agtaaagcgg ccgcgactcg aattcgatat caagcttatc
     5821 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     5881 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     5941 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     6001 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     6061 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     6121 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     6181 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     6241 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     6301 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     6361 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac
     6421 cgtcgacctc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc
     6481 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca
     6541 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     6601 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat
     6661 ctgtggatct accacacaca aggctacttc cctgattggc agaactacac accagggcca
     6721 gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag
     6781 aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat
     6841 gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt
     6901 catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc
     6961 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7021 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7081 ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc
     7141 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     7201 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     7261 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     7321 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     7381 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     7441 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     7501 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     7561 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     7621 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     7681 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     7741 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     7801 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     7861 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     7921 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     7981 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     8041 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     8101 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     8161 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     8221 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
     8281 acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg
     8341 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact
     8401 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg
     8461 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg
     8521 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg
     8581 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg
     8641 gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt
     8701 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     8761 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     8821 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     8881 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     8941 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     9001 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     9061 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     9121 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     9181 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     9241 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     9301 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     9361 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     9421 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     9481 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     9541 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     9601 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     9661 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     9721 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     9781 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     9841 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     9901 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     9961 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    10021 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    10081 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    10141 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    10201 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    10261 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    10321 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    10381 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    10441 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    10501 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    10561 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    10621 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    10681 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    10741 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    10801 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    10861 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    10921 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    10981 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    11041 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    11101 cgcgcacatt tccccgaaaa gtgccacctg ac
//
