LOCUS       sequence-11328-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Bonano VI, Oltean S, Garcia-Blanco MA
  TITLE     A protocol for imaging alternative splicing regulation in vivo using
            fluorescence reporters in transgenic mice.
  JOURNAL   Nat Protoc. 2007  . 2(9):2166-81.
  PUBMED    17853873
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Protoc.
            2007  . 2(9):2166-81."
COMMENT     Sequence Label: pRint
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(143..161)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(193..213)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     536..555
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     730..753
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 aagcttacca tggtggcctc ctccgaggac gtcatcaaag agttcatgcg cttcaaggtg
       61 cgcatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
      121 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc
      181 gcctgggaca tcctgtcccc ccagttccag tacggctcca aggcgtacgt gaagcacccc
      241 gccgacatcc ccgactacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
      301 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
      361 acgctgatct acaaggtgag tatggatccc tctaaaagcg ggcatgactt ctagagtagt
      421 ccagggtttc cgagggtttc cgtcgactca gctcgtctcg agggcgtact aactgggccc
      481 tttccctttt ttttcctcag gtgaagttcc gcggcaccaa cttccccccc gacggccccg
      541 taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg
      601 gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc cactacctgg
      661 tggagttcaa gaccatctac atggccaaga agcccgtgca gctgcccggc tactactacg
      721 tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
      781 agcgctccga gggccgccac cacctgttcc tgtagaccgg t
//
