LOCUS       sequence-11345-e        4152 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Barkan D, Rao V, Sukenick GD, Glickman MS
  TITLE     Redundant function of cmaA2 and mmaA2 in Mycobacterium tuberculosis 
            cis cyclopropanation of oxygenated mycolates.
  JOURNAL   J Bacteriol. 2010 Jul . 192(14):3661-8.
  PUBMED    20472794
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol. 2010 Jul . 192(14):3661-8."
COMMENT     Sequence Label: pMSG360
FEATURES             Location/Qualifiers
     source          1..4152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    96..129
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    1652..1685
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    2044..2178
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      2352..2940
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2841..2860
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3105..3122
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    3336..3470
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ctagtgggca gatcttcgaa tgcatcgcgc gcaccgtacg tctcgaggaa ttcctgcagg
       61 atatctggat ccacgaagct tcatggtggc ccggtataac ttcgtataat gtatgctata
      121 cgaagttatg gatcatcctg agcttgcatg cctgcaggtc gtcgaggtcc acaggccctc
      181 ggcgacgttc cgccgggcct cggcgaccgc cgcgtcgagg cgccggtcga aggggcagtc
      241 ctccacgggc agctcgtgga gggcgcgggc cagctccgcc atcgcctcga ccacggcgaa
      301 ccgctggtgc tcgggccact cctcggccgc cgcgacgccg gggacggcct ccgtgacgag
      361 ccacgcggcg gtgtcgtcgg caccgcgctc gacgacgcgg gggacgggga tcggcggggc
      421 ctgggacggc gcctcgccgt cgcagaacca ggcggtggcg tacaccgtcg cctcggtcgg
      481 cccgtagaga ttggcgatcc cgaccgcagc accaccgaga acgtccccga cgtggccgac
      541 cagcccgtca tcgtcaacgc ctgaccgcgg tgcggacagg ccgtgtcgcg accggccgtg
      601 cggaattaag ccggcccgta ccctgtgaat agaggtccgc tgtgacacaa gaatccctgt
      661 tacttctcga ccgtattgat tcggatgatt cctacgcgag cctgcggaac gaccaggaat
      721 tctgggagcc gctggcccgc cgagccctgg aggagctcgg gctgccggtg ccgccggtgc
      781 tgcgggtgcc cggcgagagc accaaccccg tactggtcgg cgagcccgac ccggtcatca
      841 agctgttcgg cgagcactgg tgcggtccgg agagcctcgc gtcggagtcg gaggcgtacg
      901 cggtcctggc ggacgccccg gtgccggtgc cccgcctcct cggccgcggc gagctgcggc
      961 ccggcaccgg agcctggccg tggccctacc tggtgatgag ccggatgacc ggcaccacct
     1021 ggcggtccgc gatggacggc acgaccgacc ggaacgcgct gctcgccctg gcccgcgaac
     1081 tcggccgggt gctcggccgg ctgcacaggg tgccgctgac cgggaacacc gtgctcaccc
     1141 cccattccga ggtcttcccg gaactgctgc gggaacgccg cgcggcgacc gtcgaggacc
     1201 accgcgggtg gggctacctc tcgccccggc tgctggaccg cctggaggac tggctgccgg
     1261 acgtggacac gctgctggcc ggccgcgaac cccggttcgt ccacggcgac ctgcacggga
     1321 ccaacatctt cgtggacctg gccgcgaccg aggtcaccgg gatcgtcgac ttcaccgacg
     1381 tctatgcggg agactcccgc tacagcctgg tgcaactgca tctcaacgcc ttccggggcg
     1441 accgcgagat cctggccgcg ctgctcgacg gggcgcagtg gaagcggacc gaggacttcg
     1501 cccgcgaact gctcgccttc accttcctgc acgacttcga ggtgttcgag gagaccccgc
     1561 tggatctctc cggcttcacc gatccggagg aactggcgca gttcctctgg gggccgccgg
     1621 acaccgcccc cggcgcctga cgccccagct tataacttcg tataatgtat gctatacgaa
     1681 gttattcgaa ctgtaccgga tttcggacag gactctagag gatccccggg tacctgagag
     1741 ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
     1801 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt
     1861 tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg
     1921 gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg
     1981 agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt
     2041 tcgaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     2101 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     2161 tcttatcatg tctggatctg acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg
     2221 ctaggctctt aagtaccgaa ttctgcaggt ttgacagctt atcatcgcga taagctcatg
     2281 accaaaatcc cttaacgtga gttttcgttc gactgagcgt cagaccccgt agaaaatatc
     2341 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2401 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2461 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     2521 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2581 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2641 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     2701 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     2761 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     2821 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     2881 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     2941 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctggcctt
     3001 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     3061 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     3121 aggaagcgga agagcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag
     3181 accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc
     3241 tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc
     3301 ctcaacgaca ggagcacgat catgcgcacc cgtcagatcc agacatgata agatacattg
     3361 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     3421 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt cgcgaattga
     3481 taattattat catttgcggg tcctttccgg cgatccgcct tgttacgggg cggcgacctc
     3541 gcgggttttc gctatttatg aaaattttcc ggtttaaggc gtttccgttc ttcttcgtca
     3601 taacttaatg tttttattta aaataccctc tgaaaagaaa ggaaacgaca ggtgctgaaa
     3661 gcgagctttt tggcctctgt cgtttccttt ctctgttttt gtccgtggaa tgaacaatgg
     3721 aagtcaacaa aaagcaagct agcttatcga ttactatcga cggcactgct gccagataac
     3781 accaccgggg aaacattcca tcatgatggc cgtgcggaca taggaagcca gttcatccat
     3841 cgctttcttg tctgctgcca tttgctttgt gacatccagc gccgcacatt cagcagcgtt
     3901 tttcagcgcg ttttcgatca acgtttcaat gttggtatca acaccaggtt taactttgaa
     3961 cttatcggca ctgacggtta ccttgttctg cgctggctca tcacgcagga taccaaggct
     4021 gatgttgtag atattggtca ccggctgagg gttttcgatt gccgctgcgt ggatagcacc
     4081 atttgcgatc aggcngtcct tgatgaatga cactccattg cgaataagtt cgaaggagac
     4141 ggtgtcacga aa
//
