LOCUS       sequence-11346-e        3267 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Barkan D, Rao V, Sukenick GD, Glickman MS
  TITLE     Redundant function of cmaA2 and mmaA2 in Mycobacterium tuberculosis 
            cis cyclopropanation of oxygenated mycolates.
  JOURNAL   J Bacteriol. 2010 Jul . 192(14):3661-8.
  PUBMED    20472794
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol. 2010 Jul . 192(14):3661-8."
COMMENT     Sequence Label: pMSG360zeo
FEATURES             Location/Qualifiers
     source          1..3267
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    97..130
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        join(239..244,245..262,263..269)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    275..294
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     regulatory      335..344
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             341..715
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     protein_bind    767..800
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    1159..1293
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      1467..2055
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1956..1975
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2220..2237
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    2451..2585
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ctagtgggca gatcttcgaa tgcatcgcgc gcaccgtacg tctcgaggaa ttcctgcagg
       61 atatctggat ccacgaagct tgcatggtgg cccggtataa cttcgtataa tgtatgctat
      121 acgaagttat ggatcatcct gagcttgcat gcctgcaggt cgtcgaggtc caccaagccc
      181 tcggcgacgt tccgccgggc ctcggcgacc gccgcgtcga ggcctccaga ccccaggctt
      241 gacactttat gcttccggct cgtataatgt gtggaattgt gagcgctcac aattcgcatg
      301 ctgataaatg cttcaataat agcacgtgag gagggccacc atggccaagt tgaccagtgc
      361 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
      421 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
      481 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
      541 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
      601 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
      661 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
      721 gcctcgagat aaaattgcag gaattcgatg cctgacgccc cagcttataa cttcgtataa
      781 tgtatgctat acgaagttat tcgaactgta ccggatttcg gacaggactc tagaggatcc
      841 ccgggtacct gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta
      901 tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag
      961 cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt
     1021 cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca
     1081 ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct
     1141 acgtcttgct ggcgttcgaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     1201 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     1261 aaactcatca atgtatctta tcatgtctgg atctgacggg tgcgcatgat cgtgctcctg
     1321 tcgttgagga cccggctagg ctcttaagta ccgaattctg caggtttgac agcttatcat
     1381 cgcgataagc tcatgaccaa aatcccttaa cgtgagtttt cgttcgactg agcgtcagac
     1441 cccgtagaaa atatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1501 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1561 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     1621 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1681 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1741 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1801 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1861 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1921 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1981 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     2041 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     2101 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2161 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2221 gcgagtcagt gagcgaggaa gcggaagagc gctgacttcc gcgtttccag actttacgaa
     2281 acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag
     2341 tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc
     2401 cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtca gatccagaca
     2461 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     2521 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     2581 aagttcgcga attgataatt attatcattt gcgggtcctt tccggcgatc cgccttgtta
     2641 cggggcggcg acctcgcggg ttttcgctat ttatgaaaat tttccggttt aaggcgtttc
     2701 cgttcttctt cgtcataact taatgttttt atttaaaata ccctctgaaa agaaaggaaa
     2761 cgacaggtgc tgaaagcgag ctttttggcc tctgtcgttt cctttctctg tttttgtccg
     2821 tggaatgaac aatggaagtc aacaaaaagc aagctagctt atcgattact atcgacggca
     2881 ctgctgccag ataacaccac cggggaaaca ttccatcatg atggccgtgc ggacatagga
     2941 agccagttca tccatcgctt tcttgtctgc tgccatttgc tttgtgacat ccagcgccgc
     3001 acattcagca gcgtttttca gcgcgttttc gatcaacgtt tcaatgttgg tatcaacacc
     3061 aggtttaact ttgaacttat cggcactgac ggttaccttg ttctgcgctg gctcatcacg
     3121 caggatacca aggctgatgt tgtagatatt ggtcaccggc tgagggtttt cgattgccgc
     3181 tgcgtggata gcaccatttg cgatcaggcn gtccttgatg aatgacactc cattgcgaat
     3241 aagttcgaag gagacggtgt cacgaaa
//
