LOCUS       sequence-11352-e        2436 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Poltorak A, He X, Smirnova I, Liu MY, Van Huffel C, Du X, Birdwell 
            D, Alejos E, Silva M, Galanos C, Freudenberg M, Ricciardi-Castagnoli
            P, Layton B, Beutler B
  TITLE     Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations 
            in Tlr4 gene.
  JOURNAL   Science. 1998 Dec 11. 282(5396):2085-8.
  PUBMED    9851930
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            1998 Dec 11. 282(5396):2085-8."
COMMENT     Sequence Label: p3xFLAG-CMV-Tlr4
FEATURES             Location/Qualifiers
     source          1..2436
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1768..1776
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ttgaatccct gcatagaggt agttcctaat attacctacc aatgcatgga tcagaaactc
       61 agcaaagtcc ctgatgacat tccttcttca accaagaaca tagatctgag cttcaacccc
      121 ttgaagatct taaaaagcta tagcttctcc aatttttcag aacttcagtg gctggattta
      181 tccaggtgtg aaattgaaac aattgaagac aaggcatggc atggcttaca ccacctctca
      241 aacttgatac tgacaggaaa ccctatccag agtttttccc caggaagttt ctctggacta
      301 acaagtttag agaatctggt ggctgtggag acaaaattgg cctctctaga aagcttccct
      361 attggacagc ttataacctt aaagaaactc aatgtggctc acaattttat acattcctgt
      421 aagttacctg catatttttc caatctgacg aacctagtac atgtggatct ttcttataac
      481 tatattcaaa ctattactgt caacgactta cagtttctac gtgaaaatcc acaagtcaat
      541 ctctctttag acatgtcttt gaacccaatt gacttcattc aagaccaagc ctttcaggga
      601 attaagctcc atgaactgac tctaagaggt aattttaata gctcaaatat aatgaaaact
      661 tgccttcaaa acctggctgg tttacacgtc catcggttga tcttgggaga atttaaagat
      721 gaaaggaatc tggaaatttt tgaaccctct atcatggaag gactatgtga tgtgaccatt
      781 gatgagttca ggttaacata tacaaatgat ttttcagatg atattgttaa gttccattgc
      841 ttggcgaatg tttctgcaat gtctctggca ggtgtatcta taaaatatct agaagatgtt
      901 cctaaacatt tcaaatggca atccttatca atcattagat gtcaacttaa gcagtttcca
      961 actctggatc taccctttct taaaagtttg actttaacta tgaacaaagg gtctatcagt
     1021 tttaaaaaag tggccctacc aagtctcagc tatctagatc ttagtagaaa tgcactgagc
     1081 tttagtggtt gctgttctta ttctgatttg ggaacaaaca gcctgagaca cttagacctc
     1141 agcttcaatg gtgccatcat tatgagtgcc aatttcatgg gtctagaaga gctgcagcac
     1201 ctggattttc agcactctac tttaaaaagg gtcacagaat tctcagcgtt cttatccctt
     1261 gaaaagctac tttaccttga catctcttat actaacacca aaattgactt cgatggtata
     1321 tttcttggct tgaccagtct caacacatta aaaatggctg gcaattcttt caaagacaac
     1381 accctttcaa atgtctttgc aaacacaaca aacttgacat tcctggatct ttctaaatgt
     1441 caattggaac aaatatcttg gggggtattt gacaccctcc atagacttca attattaaat
     1501 atgagtcaca acaatctatt gtttttggat tcatcccatt ataaccagct gtattccctc
     1561 agcactcttg attgcagttt caatcgcata gagacatcta aaggaatact gcaacatttt
     1621 ccaaagagtc tagccttctt caatcttact aacaattctg ttgcttgtat atgtgaacat
     1681 cagaaattcc tgcagtgggt caaggaacag aagcagttct tggtgaatgt tgaacaaatg
     1741 acatgtgcaa cacctgtaga gatgaatacc tccttagtgt tggattttaa taattctacc
     1801 tgttatatgt acaagacaat catcagtgtg tcagtggtca gtgtgattgt ggtatccact
     1861 gtagcatttc tgatatacca cttctatttt cacctgatac ttattgctgg ctgtaaaaag
     1921 tacagcagag gagaaagcat ctatgatgca tttgtgatct actcgagtca gaatgaggac
     1981 tgggtgagaa atgagctggt aaagaattta gaagaaggag tgccccgctt tcacctctgc
     2041 cttcactaca gagactttat tcctggtgta gccattgctg ccaacatcat ccaggaaggc
     2101 ttccacaaga gccggaaggt tattgtggta gtgtctagac actttattca gagccgttgg
     2161 tgtatctttg aatatgagat tgctcaaaca tggcagtttc tgagcagccg ctctggcatc
     2221 atcttcattg tccttgagaa ggttgagaag tccctgctga ggcagcaggt ggaattgtat
     2281 cgccttctta gcagaaacac ctacctggaa tgggaggaca atcctctggg gaggcacatc
     2341 ttctggagaa gacttaaaaa tgccctattg gatggaaaag cctcgaatcc tgagcaaaca
     2401 gcagaggaag aacaagaaac ggcaacttgg acctga
//
