LOCUS       sequence-11357-e        9061 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9061)
  AUTHORS   Fuerer C, Nusse R
  TITLE     Lentiviral vectors to probe and manipulate the wnt signaling 
            pathway.
  JOURNAL   PLoS One. 2010  . 5(2):e9370.
  PUBMED    20186325
REFERENCE   2  (bases 1 to 9061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2010  . 5(2):e9370."
COMMENT     Sequence Label: 7TFP
FEATURES             Location/Qualifiers
     source          1..9061
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        201..232
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      288..297
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             294..1946
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(360..378)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     primer_bind     complement(1965..1985)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1970..2299
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2150..2285
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2309..2908
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(2309..2328)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2805..2825
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2970..3558
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3023..3043)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3441..3452)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3645..3878
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3950..4071
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3987..4006)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4041..4060
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4090..4225
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4257..4276)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4258..4276)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4286..4303)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4286..4302)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4295..4317)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4447..4902
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4534..4553)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4744..4765
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4931..5035
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5036..5104,5105..5896)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5254..5273)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6067..6655
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6556..6575
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6809..6826
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6943..6964
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6979..6984,6985..7002,7003..7009)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7017..7033
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7022..7044
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7041..7057
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7041..7057
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7076..7096
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7078..7096
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             7352..7532
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7576..7701
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8194..8427
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8612..8656
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8923..9040
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctagcccggg ctcgagcagc tgaagcttgc atgcctgcag ggtaccgagc tcttacgcga
       61 gatcaaaggg ggtaagatca aagggggtaa gatcaaaggg gcgcgagatc aaagggggta
      121 agatcaaagg gggtaagatc aaagggggta agatcaaagg ggcgcgcccg cgtgctagcc
      181 cgggctcgag atctagactc tagagggtat ataatggaag ctcgaattcc agcttggcat
      241 tccggtactg ttggtaaaaa gcttggcatt ccggtactgt tggtaaagcc accatggaag
      301 acgccaaaaa cataaagaaa ggcccggcgc cattctatcc gctggaagat ggaaccgctg
      361 gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca attgctttta
      421 cagatgcaca tatcgaggtg gacatcactt acgctgagta cttcgaaatg tccgttcggt
      481 tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc gtatgcagtg
      541 aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga gttgcagttg
      601 cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgggc atttcgcagc
      661 ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg caaaaaaagc
      721 tcccaatcat ccaaaaaatt attatcatgg attctaaaac ggattaccag ggatttcagt
      781 cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac gattttgtgc
      841 cagagtcctt cgatagggac aagacaattg cactgatcat gaactcctct ggatctactg
      901 gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg cgtgagattc tcgcatgcca
      961 gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt gttgttccat
     1021 tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga tttcgagtcg
     1081 tcttaatgta tagatttgaa gaagagctgt ttctgaggag ccttcaggat tacaagattc
     1141 aaagtgcgct gctggtgcca accctattct ccttcttcgc caaaagcact ctgattgaca
     1201 aatacgattt atctaattta cacgaaattg cttctggtgg cgctcccctc tctaaggaag
     1261 tcggggaagc ggttgccaag aggttccatc tgccaggtat caggcaagga tatgggctca
     1321 ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg ggcgcggtcg
     1381 gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg aaaacgctgg
     1441 gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc ggttatgtaa
     1501 acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat tctggagaca
     1561 tagcttactg ggacgaagac gaacacttct tcatcgttga ccgcctgaag tctctgatta
     1621 agtacaaagg ctatcaggtg gctcccgctg aattggaatc catcttgctc caacacccca
     1681 acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga cgccggtgaa cttcccgccg
     1741 ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg gattacgtcg
     1801 ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg gacgaagtac
     1861 cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc ataaaggcca
     1921 agaagggcgg aaagatcgcc gtgtaattct agagtcgacc ctgtggaatg tgtgtcagtt
     1981 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     2041 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     2101 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     2161 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     2221 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     2281 aggcctaggc ttttgcaaaa agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
     2341 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     2401 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     2461 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     2521 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     2581 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     2641 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     2701 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     2761 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     2821 cgagcggctc ggcttcaccg tcaccgccga cgtcgagtgc ccgaaggacc gcgcgacctg
     2881 gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga
     2941 aaggagcgca cgaccccatg ccagtcgaca atcaacctct ggattacaaa atttgtgaaa
     3001 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     3061 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     3121 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     3181 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     3241 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     3301 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     3361 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     3421 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     3481 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     3541 tttgggccgc ctccccgcct ggaattcgag ctcggtacct ttaagaccaa tgacttacaa
     3601 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     3661 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     3721 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     3781 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     3841 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     3901 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     3961 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     4021 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     4081 gctctagcta tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     4141 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     4201 tagtgaggag gcttttttgg aggcctaggc ttttgcgtcg agacgtaccc aattcgccct
     4261 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     4321 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     4381 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     4441 ggcgcgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     4501 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     4561 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     4621 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     4681 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     4741 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     4801 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     4861 aatttaacgc gaattttaac aaaatattaa cgtttacaat ttcccaggtg gcacttttcg
     4921 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     4981 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     5041 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     5101 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     5161 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     5221 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     5281 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     5341 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     5401 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     5461 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     5521 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     5581 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     5641 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     5701 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     5761 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     5821 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     5881 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     5941 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     6001 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     6061 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     6121 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     6181 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     6241 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     6301 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     6361 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     6421 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     6481 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     6541 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     6601 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     6661 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     6721 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     6781 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     6841 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     6901 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     6961 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     7021 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
     7081 taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata
     7141 ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa
     7201 aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg
     7261 caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt
     7321 gtatttaagt gcctagctcg atacaataaa cgggtctctc tggttagacc agatctgagc
     7381 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7441 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7501 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cctgaaagcg
     7561 aaagggaaac cagagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     7621 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     7681 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     7741 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     7801 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     7861 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     7921 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     7981 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     8041 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     8101 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     8161 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     8221 agcagcagga agcactatgg gcgcagcctc aatgacgctg acggtacagg ccagacaatt
     8281 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     8341 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     8401 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     8461 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     8521 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     8581 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     8641 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     8701 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     8761 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     8821 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     8881 atccattcga ttagtgaacg gatctcgacg gtatcggtta acttttaaaa gaaaaggggg
     8941 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     9001 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt atcgatcacg agactagcct
     9061 c
//
