LOCUS       sequence-11359-e       10244 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10244)
  AUTHORS   Fuerer C, Nusse R
  TITLE     Lentiviral vectors to probe and manipulate the wnt signaling 
            pathway.
  JOURNAL   PLoS One. 2010  . 5(2):e9370.
  PUBMED    20186325
REFERENCE   2  (bases 1 to 10244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2010  . 5(2):e9370."
COMMENT     Sequence Label: EdTC
FEATURES             Location/Qualifiers
     source          1..10244
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    7..595
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(60..80)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(478..489)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             682..915
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    987..1108
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1024..1043)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1078..1097
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1127..1262
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(1295..1313)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1323..1340)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1323..1339)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1332..1354)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1484..1939
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1571..1590)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1781..1802
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1968..2072
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2073..2141,2142..2933)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2291..2310)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3104..3692
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3593..3612
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3846..3863
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3980..4001
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4016..4021,4022..4039,4040..4046)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4054..4070
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4059..4081
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4078..4094
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4078..4094
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4113..4133
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4115..4133
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             4389..4569
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4613..4738
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5231..5464
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5648..5692
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5953..6070
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6106..7284
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6337..7275
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7232..7252
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        7301..7319
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7403..7426
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(9191..9211)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9196..9524
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             9534..10244
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(9679..9697)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     9947..9966
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
       61 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
      121 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
      181 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
      241 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
      301 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
      361 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
      421 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
      481 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
      541 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga
      601 attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
      661 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
      721 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
      781 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
      841 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
      901 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
      961 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     1021 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     1081 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     1141 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     1201 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     1261 cctaggcttt tgcgtcgaga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     1321 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     1381 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     1441 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcgacgcgcc ctgtagcggc
     1501 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     1561 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     1621 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     1681 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     1741 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     1801 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     1861 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     1921 atattaacgt ttacaatttc ccaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     1981 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     2041 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     2101 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     2161 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     2221 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     2281 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     2341 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     2401 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     2461 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     2521 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     2581 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     2641 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     2701 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     2761 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     2821 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     2881 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     2941 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     3001 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     3061 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3121 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     3181 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3241 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3301 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3361 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3421 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     3481 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     3541 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     3601 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     3661 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     3721 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     3781 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     3841 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     3901 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     3961 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     4021 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     4081 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
     4141 agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
     4201 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
     4261 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
     4321 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata
     4381 caataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     4441 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     4501 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     4561 tctctagcag tggcgcccga acagggacct gaaagcgaaa gggaaaccag agctctctcg
     4621 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     4681 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     4741 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     4801 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     4861 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     4921 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     4981 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     5041 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag
     5101 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     5161 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     5221 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     5281 cagcctcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     5341 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     5401 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     5461 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     5521 ctagttggag taataaatct ctggaacaga ttggaatcac acgacctgga tggagtggga
     5581 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     5641 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     5701 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     5761 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     5821 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     5881 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     5941 tcgacggtta acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     6001 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     6061 tcaaaatttt atcggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt
     6121 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     6181 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     6241 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     6301 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
     6361 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg
     6421 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct
     6481 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
     6541 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
     6601 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
     6661 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
     6721 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
     6781 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
     6841 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
     6901 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
     6961 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
     7021 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt
     7081 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     7141 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     7201 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     7261 tttcttccat ttcaggtgtc gtgaggaatt agcttggtac taatacgact cactataggg
     7321 agacccaagc tggctaggta agcttggtac cgagctcgga tccactagtc cagtgtggtg
     7381 gaattctgca gataacacca tggactacaa agacgatgac gataaaaagg atccgagctc
     7441 gaattccgaa aactcctcgg cagagaggga tttagctgat gtcaaatcgt ctctagtcaa
     7501 tgaatcagaa acgaatcaaa acagctcctc cgattccgag gcggaaagac ggcctccgcc
     7561 tcgctccgaa agtttccgag acaaatcccg ggaaagtttg gaagaagcgg ccaagaggca
     7621 agatggaggg ctctttaagg ggccaccgta tcccggctac cccttcatca tgatccccga
     7681 cctgacgagc ccctacctcc ccaacggatc gctctcgccc accgcccgaa cctatctcca
     7741 gatgaaatgg ccactgcttg atgtccaggc agggagcctc cagagtagac aagccctcaa
     7801 ggatgcccgg tccccatcac cggcacacat tgtctctaac aaagtgccag tggtgcagca
     7861 ccctcaccat gtccaccccc tcacgcctct tatcacgtac agcaatgaac acttcacgcc
     7921 gggaaaccca cctccacact taccagccga cgtagacccc aaaacaggaa tcccacggcc
     7981 tccgcaccct ccagatatat ccccgtatta cccactatcg cctggcaccg taggacaaat
     8041 cccccatccg ctaggatggt tagtaccaca gcaaggtcaa ccagtgtacc caatcacgac
     8101 aggaggattc agacacccct accccacagc tctgaccgtc aatgcttcca tgtccaggtt
     8161 ccctccccat atggtcccac cacatcatac gctacacacg acgggcattc cgcatccggc
     8221 catagtcaca ccaacagtca aacaggaatc gtcccagagt gatgtcggct cactccatag
     8281 ttcaaagcat caggactcca aaaaggaaga agaaaagaag aagccccaca taaagaaacc
     8341 tcttaatgca ttcatgttgt atatgaagga aatgagagca aaggtcgtag ctgagtgcac
     8401 gttgaaagaa agcgcggcca tcaaccagat ccttgggcgg aggtggcatg cactgtccag
     8461 agaagagcaa gcgaaatact acgagctggc ccggaaggag cgacagcttc atatgcaact
     8521 gtaccccggc tggtccgcgc gggataacta tggaaagaag aagaagagga aaagggacaa
     8581 gcagccggga gagaccaatg aacacagcga atgtttccta aatccttgcc tttcacttcc
     8641 tccgattaca gacctgagcg ctcctaagaa atgccgagcg cgctttggcc ttgatcaaca
     8701 gaataactgg tgcggccctt gcaggagaaa aaaaaagtgc gttcgctaca tacaaggtga
     8761 aggcagctgc ctcagcccac cctcttcaga tggaagctta ctagattcgc ctcccccctc
     8821 cccgaacctg ctaggctccc ctccccgaga cgccaagtca cagactgagc agacccagcc
     8881 tctgtcgctg tccctgaagc ccgaccccct ggcccacctg tccatgatgc ctccgccacc
     8941 cgccctcctg ctcgctgagg ccacccacaa ggcctccgcc ctctgtccca acggggccct
     9001 ggacctgccc ccagccgctt tgcagcctgc cgccccctcc tcatcaattg cacagccgtc
     9061 gacttcttcc ttacattccc acagctccct ggccgggacc cagccccagc cgctgtcgct
     9121 cgtcaccaag tctttagaat aggctagagg atccggacta gttaacgcgt cgaccagtgg
     9181 tcgaccctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     9241 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     9301 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     9361 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     9421 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     9481 cagaagtagt gaggaggctt ttttggagcc taggcttttg caaaaagctt accatggtga
     9541 gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
     9601 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     9661 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct
     9721 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg
     9781 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     9841 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
     9901 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
     9961 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga
    10021 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca
    10081 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca
    10141 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
    10201 agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaa
//
