LOCUS       sequence-11362-e       10792 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10792)
  AUTHORS   Fuerer C, Nusse R
  TITLE     Lentiviral vectors to probe and manipulate the wnt signaling 
            pathway.
  JOURNAL   PLoS One. 2010  . 5(2):e9370.
  PUBMED    20186325
REFERENCE   2  (bases 1 to 10792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2010  . 5(2):e9370."
COMMENT     Sequence Label: E[beta]P
FEATURES             Location/Qualifiers
     source          1..10792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    10..598
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(63..83)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(481..492)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             685..918
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    990..1111
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1027..1046)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1081..1100
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1130..1265
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(1298..1316)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1326..1343)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1326..1342)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1335..1357)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1487..1942
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1574..1593)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1784..1805
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1971..2075
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2076..2144,2145..2936)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2294..2313)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3107..3695
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3596..3615
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3849..3866
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3983..4004
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4019..4024,4025..4042,4043..4049)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4057..4073
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4062..4084
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4081..4097
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4081..4097
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4116..4136
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4118..4136
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             4392..4572
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4616..4741
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5234..5467
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5651..5695
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5956..6073
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6109..7287
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6340..7278
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7235..7255
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        7304..7322
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7448..7483
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
     primer_bind     complement(9797..9817)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9802..10131
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9982..10117
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10141..10740
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(10141..10160)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10637..10657
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 ccagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
       61 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
      121 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
      181 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
      241 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
      301 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
      361 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
      421 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
      481 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
      541 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
      601 ggaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
      661 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
      721 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
      781 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
      841 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
      901 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
      961 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     1021 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     1081 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     1141 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     1201 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     1261 aggcctaggc ttttgcgtcg agacgtaccc aattcgccct atagtgagtc gtattacgcg
     1321 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     1381 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     1441 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcgacgc gccctgtagc
     1501 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     1561 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     1621 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     1681 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     1741 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     1801 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     1861 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     1921 aaaatattaa cgtttacaat ttcccaggtg gcacttttcg gggaaatgtg cgcggaaccc
     1981 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     2041 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     2101 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     2161 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     2221 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     2281 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     2341 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     2401 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     2461 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     2521 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     2581 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     2641 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     2701 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     2761 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     2821 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     2881 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     2941 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     3001 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     3061 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3121 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3181 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3241 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3301 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3361 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3421 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3481 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3541 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3601 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3661 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3721 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     3781 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     3841 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     3901 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     3961 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4021 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4081 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
     4141 aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat
     4201 ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt
     4261 ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg
     4321 gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg
     4381 atacaataaa cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     4441 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     4501 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     4561 aaatctctag cagtggcgcc cgaacaggga cctgaaagcg aaagggaaac cagagctctc
     4621 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     4681 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     4741 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     4801 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     4861 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     4921 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     4981 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     5041 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     5101 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     5161 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     5221 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     5281 gcgcagcctc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     5341 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     5401 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     5461 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     5521 atgctagttg gagtaataaa tctctggaac agattggaat cacacgacct ggatggagtg
     5581 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     5641 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     5701 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     5761 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     5821 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     5881 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     5941 atctcgacgg ttaactttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     6001 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     6061 aattcaaaat tttatcggac cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc
     6121 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
     6181 aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
     6241 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
     6301 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc
     6361 cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg
     6421 ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg
     6481 ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg
     6541 ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct
     6601 ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt
     6661 gtaaatgcgg gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac
     6721 ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg
     6781 agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg
     6841 ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg
     6901 gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg
     6961 ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc
     7021 gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc
     7081 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac
     7141 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa
     7201 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt
     7261 ttttttcttc catttcaggt gtcgtgagga attagcttgg tactaatacg actcactata
     7321 gggagaccca agctggctag gtaagcttgg taccgagctc ggatccacta gtccagtgtg
     7381 gtggaattcc cgggataaca ccatggctac tcaagctgac ctgatggagt tggacatggc
     7441 catggagccg gacagaaaag ctgctgtcag ccactggcag cagcagtctt acttggatgc
     7501 tggaatccat gctggtgcca ccgccacagc tcctgccctg agtggcaagg gcaaccctga
     7561 ggaagaagat gttgacacct cccaagtcct ttatgaatgg gagcaaggct tttcccagtc
     7621 cttcacgcaa gagcaagtag ctgatattga cgggcagtat gcaatgacta gggctcagag
     7681 ggtccgagct gccatgttcc ctgagacgct agatgagggc atgcagatcc catccacgca
     7741 gtttgacgct gctcatccca ctaatgtcca gcgcttggct gaaccatcac agatgttgaa
     7801 acatgcagtt gtcaatttga ttaactatca ggatgacgcg gaacttgcca cacgtgcaat
     7861 tcctgagctg acaaaactgc taaacgatga ggaccaggtg gtagttaata aagctgctgt
     7921 tatggtccat cagctttcca aaaaggaagc ttccagacat gccatcatgc gctcccctca
     7981 gatggtgtct gccattgtac gcaccatgca gaatacaaat gatgtagaga cagctcgttg
     8041 tactgctggg actctgcaca acctttctca ccaccgcgag ggcttgctgg ccatctttaa
     8101 gtctggtggc atcccagcgc tggtgaaaat gcttgggtca ccagtggatt ctgtactgtt
     8161 ctacgccatc acgacactgc ataatctcct gctccatcag gaaggagcta aaatggcagt
     8221 gcgcctagct ggtggactgc agaaaatggt tgctttgctc aacaaaacaa acgtgaaatt
     8281 cttggctatt acaacagact gccttcagat cttagcttat ggcaatcaag agagcaagct
     8341 catcattctg gccagtggtg gaccccaagc cttagtaaac ataatgagga cctacactta
     8401 tgagaagctt ctgtggacca caagcagagt gctgaaggtg ctgtctgtct gctctagcaa
     8461 caagccggcc attgtagaag ctggtgggat gcaggcactg gggcttcatc tgacagaccc
     8521 aagtcagcga cttgttcaaa actgtctttg gactctcaga aacctttcag atgcagcgac
     8581 taagcaggaa gggatggaag gcctccttgg gactctagtg cagcttctgg gttccgatga
     8641 tataaatgtg gtcacctgtg cagctggaat tctctctaac ctcacttgca ataattacaa
     8701 aaacaagatg atggtgtgcc aagtgggtgg catagaggct cttgtacgca ccgtccttcg
     8761 tgctggtgac agggaagaca tcactgagcc tgccatctgt gctcttcgtc atctgaccag
     8821 ccggcatcag gaagccgaga tggcccagaa tgccgttcgc cttcattatg gactgcctgt
     8881 tgtggttaaa ctcctgcacc caccatccca ctggcctctg ataaaggcaa ctgttggatt
     8941 gattcgaaac cttgcccttt gcccagcaaa tcatgcgcct ttgcgggaac agggtgctat
     9001 tccacgacta gttcagctgc ttgtacgagc acatcaggac acccaacggc gcacctccat
     9061 gggtggaacg cagcagcagt ttgtggaggg cgtgcgcatg gaggagatag tagaagggtg
     9121 tactggagct ctccacatcc ttgctcggga cgttcacaac cggattgtaa tccgaggact
     9181 caataccatt ccattgtttg tgcagttgct ttattctccc attgaaaata tccaaagagt
     9241 agctgcaggg gtcctctgtg aacttgctca ggacaaggag gctgcagagg ccattgaagc
     9301 tgagggagcc acagctcccc tgacagagtt actccactcc aggaatgaag gcgtggcaac
     9361 atacgcagct gctgtcctat tccgaatgtc tgaggacaag ccacaggatt acaagaagcg
     9421 gctttcagtc gagctgacca gttccctctt caggacagag ccaatggctt ggaatgagac
     9481 tgcagatctt ggactggaca ttggtgccca gggagaagcc cttggatatc gccaggatga
     9541 tcccagctac cgttcttttc actctggtgg atacggccag gatgccttgg ggatggaccc
     9601 tatgatggag catgagatgg gtggccacca ccctggtgct gactatccag ttgatgggct
     9661 gcctgatctg ggacacgccc aggacctcat ggatgggctg cccccaggtg atagcaatca
     9721 gctggcctgg tttgatactg acctgtaatc tagaggatcc ggactagtta acgcgtcgac
     9781 cagtggtcga ccctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     9841 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     9901 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     9961 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
    10021 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
    10081 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttacc
    10141 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
    10201 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
    10261 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
    10321 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
    10381 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
    10441 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
    10501 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
    10561 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
    10621 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
    10681 gacgtcgagt gcccgaagga ccgcgcgacc tggtgcatga cccgcaagcc cggtgcctga
    10741 cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca tg
//
