LOCUS       sequence-11365-e        9826 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9826)
  AUTHORS   Kumar P, Lyle KS, Gierke S, Matov A, Danuser G, Wittmann T
  TITLE     GSK3beta phosphorylation modulates CLASP-microtubule association and
            lamella microtubule attachment.
  JOURNAL   J Cell Biol. 2009 Mar 23. 184(6):895-908.
  PUBMED    19289791
REFERENCE   2  (bases 1 to 9826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2009 Mar 23. 184(6):895-908."
COMMENT     Sequence Label: pShuttle-EGFP-tubulin
FEATURES             Location/Qualifiers
     source          1..9826
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..103
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     191..341
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     enhancer        399..702
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        703..906
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     857..877
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      946..955
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(952..954,955..957,958..1668)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(952..954,955..957,958..1668)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(997..1018)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1258..1277)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1605..1626
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3172..3293
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    3474..3595
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   6786..6888
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     primer_bind     complement(6977..6994)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7148..7736)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7228..7247)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             8568..9362
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8568..9362
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8622..8641)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9232..9251
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(9410..9789)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9472..9493)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9684..9703
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt
       61 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt
      121 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg
      181 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag
      241 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga
      301 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tactgtaata gtaatcaatt
      361 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
      421 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      481 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      541 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
      601 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
      661 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      721 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      781 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
      841 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      901 agagctggtt tagtgaaccg tcagatccgc tagagatctg gtaccgccac catggtgagc
      961 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     1021 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     1081 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     1141 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     1201 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     1261 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     1321 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     1381 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     1441 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     1501 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     1561 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     1621 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtc cggactcaga
     1681 tctcgagtgc gtgagtgcat ctccatccac gttggccagg ctggtgtcca gattggcaat
     1741 gcctgctggg agctctactg cctggaacac ggcatccagc ccgatggcca gatgccaagt
     1801 gacaagacca ttgggggagg agatgactcc ttcaacacct tcttcagtga gacgggcgct
     1861 ggcaagcacg tgccccgggc tgtgtttgta gacttggaac ccacagtcat tgatgaagtt
     1921 cgcactggca cctaccgcca gctcttccac cctgagcagc tcatcacagg caaggaagat
     1981 gctgccaata actatgcccg agggcactac accattggca aggagatcat tgaccttgtg
     2041 ttggaccgaa ttcgcaagct ggctgaccag tgcaccggtc ttcagggctt cttggttttc
     2101 cacagctttg gtgggggaac tggttctggg ttcacctccc tgctcatgga acgtctctca
     2161 gttgattatg gcaagaagtc caagctggag ttctccattt acccagcacc ccaggtttcc
     2221 acagctgtag ttgagcccta caactccatc ctcaccaccc acaccaccct ggagcactct
     2281 gattgtgcct tcatggtaga caatgaggcc atctatgaca tctgtcgtag aaacctcgat
     2341 atcgagcgcc caacctacac taaccttaac cgccttatta gccagattgt gtcctccatc
     2401 actgcttccc tgagatttga tggagccctg aatgttgacc tgacagaatt ccagaccaac
     2461 ctggtgccct acccccgcat ccacttccct ctggccacat atgcccctgt catctctgct
     2521 gagaaagcct accatgaaca gctttctgta gcagagatca ccaatgcttg ctttgagcca
     2581 gccaaccaga tggtgaaatg tgaccctcgc catggtaaat acatggcttg ctgcctgttg
     2641 taccgtggtg acgtggttcc caaagatgtc aatgctgcca ttgccaccat caaaaccaag
     2701 cgcagcatcc agtttgtgga ttggtgcccc actggcttca aggttggcat caactaccag
     2761 cctcccactg tggtgcctgg tggagacctg gccaaggtac agagagctgt gtgcatgctg
     2821 agcaacacca cagccattgc tgaggcctgg gctcgcctgg accacaagtt tgacctgatg
     2881 tatgccaagc gtgcctttgt tcactggtac gtgggtgagg ggatggagga aggcgagttt
     2941 tcagaggccc gtgaagatat ggctgccctt gagaaggatt atgaggaggt tggtgtggat
     3001 tctgttgaag gagagggtga ggaagaagga gaggaatact aaggatccac cggatctaga
     3061 taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     3121 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     3181 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     3241 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta
     3301 aattgtaagc ggccgctcga gcctaagctt ctagataaga tatccgatcc accggatcta
     3361 gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     3421 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     3481 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     3541 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg
     3601 gatctgggcg tggttaaggg tgggaaagaa tatataaggt gggggtctta tgtagttttg
     3661 tatctgtttt gcagcagccg ccgccgccat gagcaccaac tcgtttgatg gaagcattgt
     3721 gagctcatat ttgacaacgc gcatgccccc atgggccggg gtgcgtcaga atgtgatggg
     3781 ctccagcatt gatggtcgcc ccgtcctgcc cgcaaactct actaccttga cctacgagac
     3841 cgtgtctgga acgccgttgg agactgcagc ctccgccgcc gcttcagccg ctgcagccac
     3901 cgcccgcggg attgtgactg actttgcttt cctgagcccg cttgcaagca gtgcagcttc
     3961 ccgttcatcc gcccgcgatg acaagttgac ggctcttttg gcacaattgg attctttgac
     4021 ccgggaactt aatgtcgttt ctcagcagct gttggatctg cgccagcagg tttctgccct
     4081 gaaggcttcc tcccctccca atgcggttta aaacataaat aaaaaaccag actctgtttg
     4141 gatttggatc aagcaagtgt cttgctgtct ttatttaggg gttttgcgcg cgcggtaggc
     4201 ccgggaccag cggtctcggt cgttgagggt cctgtgtatt ttttccagga cgtggtaaag
     4261 gtgactctgg atgttcagat acatgggcat aagcccgtct ctggggtgga ggtagcacca
     4321 ctgcagagct tcatgctgcg gggtggtgtt gtagatgatc cagtcgtagc aggagcgctg
     4381 ggcgtggtgc ctaaaaatgt ctttcagtag caagctgatt gccaggggca ggcccttggt
     4441 gtaagtgttt acaaagcggt taagctggga tgggtgcata cgtggggata tgagatgcat
     4501 cttggactgt atttttaggt tggctatgtt cccagccata tccctccggg gattcatgtt
     4561 gtgcagaacc accagcacag tgtatccggt gcacttggga aatttgtcat gtagcttaga
     4621 aggaaatgcg tggaagaact tggagacgcc cttgtgacct ccaagatttt ccatgcattc
     4681 gtccataatg atggcaatgg gcccacgggc ggcggcctgg gcgaagatat ttctgggatc
     4741 actaacgtca tagttgtgtt ccaggatgag atcgtcatag gccattttta caaagcgcgg
     4801 gcggagggtg ccagactgcg gtataatggt tccatccggc ccaggggcgt agttaccctc
     4861 acagatttgc atttcccacg ctttgagttc agatgggggg atcatgtcta cctgcggggc
     4921 gatgaagaaa acggtttccg gggtagggga gatcagctgg gaagaaagca ggttcctgag
     4981 cagctgcgac ttaccgcagc cggtgggccc gtaaatcaca cctattaccg gctgcaactg
     5041 gtagttaaga gagctgcagc tgccgtcatc cctgagcagg ggggccactt cgttaagcat
     5101 gtccctgact cgcatgtttt ccctgaccaa atccgccaga aggcgctcgc cgcccagcga
     5161 tagcagttct tgcaaggaag caaagttttt caacggtttg agaccgtccg ccgtaggcat
     5221 gcttttgagc gtttgaccaa gcagttccag gcggtcccac agctcggtca cctgctctac
     5281 ggcatctcga tccagcatat ctcctcgttt cgcgggttgg ggcggctttc gctgtacggc
     5341 agtagtcggt gctcgtccag acgggccagg gtcatgtctt tccacgggcg cagggtcctc
     5401 gtcagcgtag tctgggtcac ggtgaagggg tgcgctccgg gctgcgcgct ggccagggtg
     5461 cgcttgaggc tggtcctgct ggtgctgaag cgctgccggt cttcgccctg cgcgtcggcc
     5521 aggtagcatt tgaccatggt gtcatagtcc agcccctccg cggcgtggcc cttggcgcgc
     5581 agcttgccct tggaggaggc gccgcacgag gggcagtgca gacttttgag ggcgtagagc
     5641 ttgggcgcga gaaataccga ttccggggag taggcatccg cgccgcaggc cccgcagacg
     5701 gtctcgcatt ccacgagcca ggtgagctct ggccgttcgg ggtcaaaaac caggtttccc
     5761 ccatgctttt tgatgcgttt cttacctctg gtttccatga gccggtgtcc acgctcggtg
     5821 acgaaaaggc tgtccgtgtc cccgtataca gacttgagag ggagtttaaa cgaattcaat
     5881 agcttgttgc atgggcggcg atataaaatg caaggtgctg ctcaaaaaat caggcaaagc
     5941 ctcgcgcaaa aaagaaagca catcgtagtc atgctcatgc agataaaggc aggtaagctc
     6001 cggaaccacc acagaaaaag acaccatttt tctctcaaac atgtctgcgg gtttctgcat
     6061 aaacacaaaa taaaataaca aaaaaacatt taaacattag aagcctgtct tacaacagga
     6121 aaaacaaccc ttataagcat aagacggact acggccatgc cggcgtgacc gtaaaaaaac
     6181 tggtcaccgt gattaaaaag caccaccgac agctcctcgg tcatgtccgg agtcataatg
     6241 taagactcgg taaacacatc aggttgattc acatcggtca gtgctaaaaa gcgaccgaaa
     6301 tagcccgggg gaatacatac ccgcaggcgt agagacaaca ttacagcccc cataggaggt
     6361 ataacaaaat taataggaga gaaaaacaca taaacacctg aaaaaccctc ctgcctaggc
     6421 aaaatagcac cctcccgctc cagaacaaca tacagcgctt ccacagcggc agccataaca
     6481 gtcagcctta ccagtaaaaa agaaaaccta ttaaaaaaac accactcgac acggcaccag
     6541 ctcaatcagt cacagtgtaa aaaagggcca agtgcagagc gagtatatat aggactaaaa
     6601 aatgacgtaa cggttaaagt ccacaaaaaa cacccagaaa accgcacgcg aacctacgcc
     6661 cagaaacgaa agccaaaaaa cccacaactt cctcaaatcg tcacttccgt tttcccacgt
     6721 tacgtcactt cccattttaa gaaaactaca attcccaaca catacaagtt actccgccct
     6781 aaaacctacg tcacccgccc cgttcccacg ccccgcgcca cgtcacaaac tccaccccct
     6841 cattatcata ttggcttcaa tccaaaataa ggtatattat tgatgatgtt aattaacatg
     6901 catggatcca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     6961 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     7021 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     7081 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7141 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     7201 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7261 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7321 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     7381 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     7441 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     7501 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     7561 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     7621 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     7681 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     7741 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     7801 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     7861 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     7921 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     7981 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     8041 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     8101 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     8161 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     8221 ctgcagccat gagattatca aaaaggatct tcacctagat ccttttcacg tagaaagcca
     8281 gtccgcagaa acggtgctga ccccggatga atgtcagcta ctgggctatc tggacaaggg
     8341 aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag
     8401 actgggcggt tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta
     8461 aggttgggaa gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc
     8521 gcaggggatc aagctctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     8581 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     8641 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     8701 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag
     8761 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     8821 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     8881 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     8941 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     9001 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     9061 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg
     9121 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     9181 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     9241 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     9301 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     9361 gaattttgtt aaaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     9421 accatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     9481 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     9541 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttgtg gtcgaggtgc
     9601 cgtaaagatc taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     9661 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     9721 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg cgcgcttaat gcgccgctac
     9781 agggcgcgtc cattcgccat tcaggatcga attaattctt aattaa
//
