LOCUS       sequence-113658-        1062 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 
            epitope tag, Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Verzijlbergen KF, Menendez-Benito V, van Welsem T, van Deventer SJ, 
            Lindstrom DL, Ovaa H, Neefjes J, Gottschling DE, van Leeuwen F
  TITLE     Recombination-induced tag exchange to track old and new proteins.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Jan 5;107(1):64-8. doi: 
            10.1073/pnas.0911164107. Epub 2009 Dec 14.
  PUBMED    20018668
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0911164107"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2010-01-5- 5"; volume: "107"; issue: "1"; pages: "64-8"
COMMENT     Sequence Label: pFvL118
FEATURES             Location/Qualifiers
     source          1..1062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    56..89
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             98..124
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      145..332
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(200..224)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     terminator      complement(451..648)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 gggcgaattg ggcccaatgc attggcgccg cggccgcggt ggatctggtg gatctataac
       61 ttcgtataat gtatgctata cgaagttatc aggtacctac ccatacgatg ttcctgacta
      121 tgcgactagt taacttctaa ataagcgaat ttcttatgat ttatgatttt tattattaaa
      181 taagttataa aaaaaataag tgtatacaaa ttttaaagtg actcttaggt tttaaaacga
      241 aaattcttat tcttgagtaa ctctttcctg taggtcaggt tgctttctca ggtatagtat
      301 gaggtcgctc ttattgacca cacctctacc ggcagatccg ctagggataa cagggtaagg
      361 atccgcattg tactgagagt gcaccataat catcgatgaa ttcgagctcg ttttcgacac
      421 tggatggcgg cgttagtatc gaatcgacag cagtatagcg accagcattc acatacgatt
      481 gacgcatgat attactttct gcgcacttaa cttcgcatct gggcagatga tgtcgaggcg
      541 aaaaaaaata taaatcacgc taacatttga ttaaaataga acaactacaa tataaaaaaa
      601 ctatacaaat gacaagttct tgaaaacaag aatcttttta ttgtcagtac tgattattcc
      661 tttgccctcg gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag
      721 ccatcggtcc agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct
      781 ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg
      841 tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc
      901 ggcgatcctg caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa
      961 gccaaccacg gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc
     1021 gcctcgctcc agtcaatgac cgctgttatg cggccattgt cc
//
