LOCUS       sequence-113659-        1006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 
            epitope tag, Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Verzijlbergen KF, Menendez-Benito V, van Welsem T, van Deventer SJ, 
            Lindstrom DL, Ovaa H, Neefjes J, Gottschling DE, van Leeuwen F
  TITLE     Recombination-induced tag exchange to track old and new proteins.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Jan 5;107(1):64-8. doi: 
            10.1073/pnas.0911164107. Epub 2009 Dec 14.
  PUBMED    20018668
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0911164107"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2010-01-5- 5"; volume: "107"; issue: "1"; pages: "64-8"
COMMENT     Sequence Label: pFvL118
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    21..77
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             complement(95..127)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     protein_bind    complement(136..169)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        264..607
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 accctcacta aagggaacaa aagcttgcat gcctgcaggt cgactctaga ggatccccgg
       61 gtaccgagct cgaattcgcg gccgcttatg tacaacccat ttgctgtcca ccagtcatgc
      121 ttgccatgtc gactgataac ttcgtatagc atacattata cgaagttatt ctagactgtg
      181 cggtatttca caccgcatag ggttaattaa ggcgcgccag atctgtttag cttgcctcgt
      241 ccccgccggg tcacccggcc agcgacatgg aggcccagaa taccctcctt gacagtcttg
      301 acgtgcgcag ctcaggggca tgatgtgact gtcgcccgta catttagccc atacatcccc
      361 atgtataatc atttgcatcc atacattttg atggccgcac ggcgcgaagc aaaaattacg
      421 gctcctcgct gcggacctgc gagcagggaa acgctcccct cacagacgcg ttgaattgtc
      481 cccacgccgc gcccctgtag agaaatataa aaggttagga tttgccactg aggttcttct
      541 ttcatatact tccttttaaa atcttgctag gatacagttc tcacatcaca tccgaacata
      601 aacaaccatg ggtaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga
      661 aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt
      721 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt
      781 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt
      841 gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg
      901 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga
      961 ggccatggat gcgatcgctg cggccgatct tagccagacg agcggg
//
