LOCUS       sequence-113661-         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombination-Induced Tag Exchange (RITE) Cassette, 3xHA to 
            3xT7-mRFP tag, Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Terweij M, van Welsem T, van Deventer S, Verzijlbergen KF, 
            Menendez-Benito V, Ontoso D, San-Segundo P, Neefjes J, van Leeuwen F
  TITLE     Recombination-induced tag exchange (RITE) cassette series to monitor
            protein dynamics in Saccharomyces cerevisiae.
  JOURNAL   G3 (Bethesda). 2013 Aug 7;3(8):1261-72. doi: 10.1534/g3.113.006213.
  PUBMED    23708297
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.113"; 
            journalName: "G3 (Bethesda)"; date: "2013-08-7- 7"; volume: "3"; 
            issue: "8"; pages: "1261-72"
COMMENT     Sequence Label: pKV014
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    66..99
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             108..134
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             141..167
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             171..197
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      218..405
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(273..297)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     terminator      complement(524..721)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 actcactata gggcgaattg ggcccaatgc attggcgccg cggccgcggt ggatctggtg
       61 gatctataac ttcgtataat gtatgctata cgaagttatc aggtacctac ccatacgatg
      121 ttcctgacta tgcgactagc tatccctatg acgtcccgga ctatgcagga tatccatatg
      181 acgttccaga ttacgctact agttaacttc taaataagcg aatttcttat gatttatgat
      241 ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta
      301 ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc
      361 tcaggtatag tatgaggtcg ctcttattga ccacacctct accggcagat ccgctaggga
      421 taacagggta aggatccaga ttgtactgag agtgcaccat aatcatcgat gaattcgagc
      481 tcgttttcga cactggatgg cggcgttagt atcgaatcga cagcagtata gcgaccagca
      541 ttcacatacg attgacgcat gatattactt tctgcgcact taacttcgca tctgggcaga
      601 tgatgtcgag gcgaaaaaaa atataaatca cgctaacatt tgattaaaat agaacaacta
      661 caatataaaa aaactataca aatgacaagt tcttgaaaac aagaatcttt ttattgtcag
      721 tactgattat tcctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag
      781 tacttctaca cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc
      841 gacagtcccg gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc
      901 atcgaaattg ccgtcaacca agctctgata gagttggtca agaccaatgc ggagca
//
