LOCUS       sequence-113668-         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombination-Induced Tag Exchange (RITE) Cassette, 3xHA to 
            3xT7-mRFP tag, Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Terweij M, van Welsem T, van Deventer S, Verzijlbergen KF, 
            Menendez-Benito V, Ontoso D, San-Segundo P, Neefjes J, van Leeuwen F
  TITLE     Recombination-induced tag exchange (RITE) cassette series to monitor
            protein dynamics in Saccharomyces cerevisiae.
  JOURNAL   G3 (Bethesda). 2013 Aug 7;3(8):1261-72. doi: 10.1534/g3.113.006213.
  PUBMED    23708297
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.113"; 
            journalName: "G3 (Bethesda)"; date: "2013-08-7- 7"; volume: "3"; 
            issue: "8"; pages: "1261-72"
COMMENT     Sequence Label: pKV014
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        8..26
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(39..713)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             complement(39..713)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(98..121)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(296..315)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     565..583
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(795..827)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     protein_bind    complement(836..869)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 agctcgaaat taaccctcac taaagggaac aaaagcttgg cgccggtgga gtggcggccc
       61 tcggcgcgct cgtactgttc cacgatggtg tagtcctcgt tgtgggaggt gatgtccagc
      121 ttgatgtcgg tcttgtaggc gccgggcagc tgcacgggct tcttggccat gtaggtggtc
      181 ttgacctcgg cgtcgtagtg gccgccgtcc ttcagcttca gcctcatctt gatctcgccc
      241 ttcagggcgc cgtcctcggg gtacatccgc tcggtggagg cctcccagcc catggtcttc
      301 ttctgcatta cggggccgtc ggaggggaag ttggtgccgc gcagcttcac cttgtagatg
      361 aactcgccgt cctgcaggga ggagtcctgg gtcacggtca ccacgccgcc gtcctcgaag
      421 ttcatcacgc gctcccactt gaagccctcg gggaaggaca gcttcaagta gtcggggatg
      481 tcggcggggt gcttcacgta ggccttggag ccgtactgga actgagggga caggatgtcc
      541 caggcgaagg gcagggggcc gcccttggtc accttcagct tggcggtctg ggtgccctcg
      601 taggggcggc cctcgccctc gccctcgatc tcgaactcgt ggccgttcac ggagccctcc
      661 atgcgcacct tgaagcgcat gaactccttg atgacgtcct cggaggaggc cattgtacat
      721 cccatttgct ggccaccggt cattgaggcc attcctccca tctgttgacc tcctgtcata
      781 gaagccatag tacaacccat ttgctgtcca ccagtcatgc ttgccatgtc gactgataac
      841 ttcgtatagc atacattata cgaagttatt ctagactgtg cggtatttca caccgcatag
      901 ggttaattaa ggcgcgccag atctgtttag cttgcctcgt ccccgccggg tcacccggcc
      961 agcgacatgg aggcccagaa
//
