LOCUS       sequence-113669-         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombination-Induced Tag Exchange (RITE) Cassette, 3xHA to 
            3xT7-mRFP tag, Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Terweij M, van Welsem T, van Deventer S, Verzijlbergen KF, 
            Menendez-Benito V, Ontoso D, San-Segundo P, Neefjes J, van Leeuwen F
  TITLE     Recombination-induced tag exchange (RITE) cassette series to monitor
            protein dynamics in Saccharomyces cerevisiae.
  JOURNAL   G3 (Bethesda). 2013 Aug 7;3(8):1261-72. doi: 10.1534/g3.113.006213.
  PUBMED    23708297
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.113"; 
            journalName: "G3 (Bethesda)"; date: "2013-08-7- 7"; volume: "3"; 
            issue: "8"; pages: "1261-72"
COMMENT     Sequence Label: pKV014
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(105..137)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     protein_bind    complement(146..179)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        274..617
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 atgacgtcct cggaggaggc cattgtacat cccatttgct ggccaccggt cattgaggcc
       61 attcctccca tctgttgacc tcctgtcata gaagccatag tacaacccat ttgctgtcca
      121 ccagtcatgc ttgccatgtc gactgataac ttcgtatagc atacattata cgaagttatt
      181 ctagactgtg cggtatttca caccgcatag ggttaattaa ggcgcgccag atctgtttag
      241 cttgcctcgt ccccgccggg tcacccggcc agcgacatgg aggcccagaa taccctcctt
      301 gacagtcttg acgtgcgcag ctcaggggca tgatgtgact gtcgcccgta catttagccc
      361 atacatcccc atgtataatc atttgcatcc atacattttg atggccgcac ggcgcgaagc
      421 aaaaattacg gctcctcgct gcggacctgc gagcagggaa acgctcccct cacagacgcg
      481 ttgaattgtc cccacgccgc gcccctgtag agaaatataa aaggttagga tttgccactg
      541 aggttcttct ttcatatact tccttttaaa atcttgctag gatacagttc tcacatcaca
      601 tccgaacata aacaaccatg ggtaaaaagc ctgaactcac cgcgacgtct gtcgagaagt
      661 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
      721 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
      781 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
      841 ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc
      901 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc
      961 cggtcgcgga ggccatgga
//
