LOCUS       sequence-113670-         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 
            epitope tag, URA selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Terweij M, van Welsem T, van Deventer S, Verzijlbergen KF, 
            Menendez-Benito V, Ontoso D, San-Segundo P, Neefjes J, van Leeuwen F
  TITLE     Recombination-induced tag exchange (RITE) cassette series to monitor
            protein dynamics in Saccharomyces cerevisiae.
  JOURNAL   G3 (Bethesda). 2013 Aug 7;3(8):1261-72. doi: 10.1534/g3.113.006213.
  PUBMED    23708297
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.113"; 
            journalName: "G3 (Bethesda)"; date: "2013-08-7- 7"; volume: "3"; 
            issue: "8"; pages: "1261-72"
COMMENT     Sequence Label: pFvL106
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    59..92
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             101..127
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      148..335
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(203..227)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        400..615
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
ORIGIN
        1 atagggcgaa ttgggcccaa tgcattggcg ccgcggccgc ggtggatctg gtggatctat
       61 aacttcgtat aatgtatgct atacgaagtt atcaggtacc tacccatacg atgttcctga
      121 ctatgcgact agttaacttc taaataagcg aatttcttat gatttatgat ttttattatt
      181 aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta ggttttaaaa
      241 cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc tcaggtatag
      301 tatgaggtcg ctcttattga ccacacctct accggcagat ccgctaggga taacagggta
      361 aggatccgag attgtactga gagtgcacca taccaccttt tcaattcatc attttttttt
      421 tattcttttt tttgatttcg gtttccttga aatttttttg attcggtaat ctccgaacag
      481 aaggaagaac gaaggaagga gcacagactt agattggtat atatacgcat atgtagtgtt
      541 gaagaaacat gaaattgccc agtattctta acccaactgc acagaacaaa aacctgcagg
      601 aaacgaagat aaatcatgtc gaaagctaca tataaggaac gtgctgctac tcatcctagt
      661 cctgttgctg ccaagctatt taatatcatg cacgaaaagc aaacaaactt gtgtgcttca
      721 ttggatgttc gtaccaccaa ggaattactg gagttagttg aagcattagg tcccaaaatt
      781 tgtttactaa aaacacatgt ggatatcttg actgattttt ccatggaggg cacagttaag
      841 ccgctaaagg cattatccgc caagtacaat tttttactct tcgaagacag aaaatttgct
      901 gacattggta atacagtcaa attgcagtac tctgcgggtg tatacagaat agcagaatgg
      961 gcagacatta cga
//
