LOCUS       sequence-113673-         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 
            epitope tag, URA selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Terweij M, van Welsem T, van Deventer S, Verzijlbergen KF, 
            Menendez-Benito V, Ontoso D, San-Segundo P, Neefjes J, van Leeuwen F
  TITLE     Recombination-induced tag exchange (RITE) cassette series to monitor
            protein dynamics in Saccharomyces cerevisiae.
  JOURNAL   G3 (Bethesda). 2013 Aug 7;3(8):1261-72. doi: 10.1534/g3.113.006213.
  PUBMED    23708297
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.113"; 
            journalName: "G3 (Bethesda)"; date: "2013-08-7- 7"; volume: "3"; 
            issue: "8"; pages: "1261-72"
COMMENT     Sequence Label: pFvL106
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        2..20
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(56..88)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     protein_bind    complement(97..130)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 aaattaaccc tcactaaagg gaacaaaagc ttgcatgcgc ggccgcttat gtacaaccca
       61 tttgctgtcc accagtcatg cttgccatgt cgactgataa cttcgtatag catacattat
      121 acgaagttat tctagactgt gcgatatttc acaccgcata gggtaataac tgatataatt
      181 aaattgaagc tctaatttgt gagtttagta tacatgcatt tacttataat acagtttttt
      241 agttttgctg gccgcatctt ctcaaatatg cttcccagcc tgcttttctg taacgttcac
      301 cctctacctt agcatccctt ccctttgcaa atagtcctct tccaacaata ataatgtcag
      361 atcctgtaga gaccacatca tccacggttc tatactgttg acccaatgcg tctcccttgt
      421 catctaaacc cacaccgggt gtcataatca accaatcgta accttcatct cttccaccca
      481 tgtctctttg agcaataaag ccgataacaa aatctttgtc gctcttcgca atgtcaacag
      541 tacccttagt atattctcca gtagataggg agcccttgca tgacaattct gctaacatca
      601 aaaggcctct aggttccttt gttacttctt ctgccgcctg cttcaaaccg ctaacaatac
      661 ctgggcccac cacaccgtgt gcattcgtaa tgtctgccca ttctgctatt ctgtatacac
      721 ccgcagagta ctgcaatttg actgtattac caatgtcagc aaattttctg tcttcgaaga
      781 gtaaaaaatt gtacttggcg gataatgcct ttagcggctt aactgtgccc tccatggaaa
      841 aatcagtcaa gatatccaca tgtgttttta gtaaacaaat tttgggacct aatgcttcaa
      901 ctaactccag taattccttg gtggtacgaa catccaatga agcacacaag tt
//
