LOCUS       sequence-113677-        2178 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  APEX2 fused to the N-terminus of alpha tubulin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, 
            Ting AY
  TITLE     Directed evolution of APEX2 for electron microscopy and proximity 
            labeling.
  JOURNAL   Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179.
  PUBMED    25419960
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179."
COMMENT     Sequence Label: APEX2 - Tubulin in pEGFP
FEATURES             Location/Qualifiers
     source          1..2178
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atggactaca aggatgacga cgataagggg aaatcatacc caacagtgtc cgcagactac
       61 caggatgccg tggagaaagc caagaagaag ctgagagggt ttatcgcaga aaagaggtgc
      121 gcacctctga tgctgagact ggctttccac agcgcaggca cctttgacaa gggaaccaaa
      181 acaggcggac cctttggaac aatcaagcac cctgctgaac tggcacatag tgccaacaat
      241 gggctggaca tcgccgtgcg gctgctggaa cctctgaaag cagagttccc aattctgtcc
      301 tacgccgatt tttatcagct ggcaggagtg gtcgctgtgg aggtcactgg gggccccaag
      361 gtgcctttcc acccaggacg ggaggacaaa ccagaaccac ctccagaggg gcgcctgcca
      421 gatccgacaa agggctccga ccatctgcga gatgtgtttg ggaaagctat gggcctgact
      481 gaccaggata tcgtcgcact gtctggaggg cacaccattg gcgccgctca taaggaaagg
      541 tcaggcttcg agggaccctg gacaagcaac cccctgattt tcgacaattc ttactttact
      601 gaactgctga gtggagagaa ggaagggctg ctgcagctgc ccagcgacaa agccctgctg
      661 tccgatcccg tgttcagacc tctggtcgat aagtatgcag ccgacgagga tgcttttttc
      721 gcagattacg cagaagcaca tcagaagctg tcagaactgg gatttgccga cgccaagggc
      781 tcgggctcga cctcgggctc gggctccgga ctcagatctc gagtgcgtga gtgcatctcc
      841 atccacgttg gccaggctgg tgtccagatt ggcaatgcct gctgggagct ctactgcctg
      901 gaacacggca tccagcccga tggccagatg ccaagtgaca agaccattgg gggaggagat
      961 gactccttca acaccttctt cagtgagacg ggcgctggca agcacgtgcc ccgggctgtg
     1021 tttgtagact tggaacccac agtcattgat gaagttcgca ctggcaccta ccgccagctc
     1081 ttccaccctg agcagctcat cacaggcaag gaagatgctg ccaataacta tgcccgaggg
     1141 cactacacca ttggcaagga gatcattgac cttgtgttgg accgaattcg caagctggct
     1201 gaccagtgca ccggtcttca gggcttcttg gttttccaca gctttggtgg gggaactggt
     1261 tctgggttca cctccctgct catggaacgt ctctcagttg attatggcaa gaagtccaag
     1321 ctggagttct ccatttaccc agcaccccag gtttccacag ctgtagttga gccctacaac
     1381 tccatcctca ccacccacac caccctggag cactctgatt gtgccttcat ggtagacaat
     1441 gaggccatct atgacatctg tcgtagaaac ctcgatatcg agcgcccaac ctacactaac
     1501 cttaaccgcc ttattagcca gattgtgtcc tccatcactg cttccctgag atttgatgga
     1561 gccctgaatg ttgacctgac agaattccag accaacctgg tgccctaccc ccgcatccac
     1621 ttccctctgg ccacatatgc ccctgtcatc tctgctgaga aagcctacca tgaacagctt
     1681 tctgtagcag agatcaccaa tgcttgcttt gagccagcca accagatggt gaaatgtgac
     1741 cctcgccatg gtaaatacat ggcttgctgc ctgttgtacc gtggtgacgt ggttcccaaa
     1801 gatgtcaatg ctgccattgc caccatcaaa accaagcgca gcatccagtt tgtggattgg
     1861 tgccccactg gcttcaaggt tggcatcaac taccagcctc ccactgtggt gcctggtgga
     1921 gacctggcca aggtacagag agctgtgtgc atgctgagca acaccacagc cattgctgag
     1981 gcctgggctc gcctggacca caagtttgac ctgatgtatg ccaagcgtgc ctttgttcac
     2041 tggtacgtgg gtgaggggat ggaggaaggc gagttttcag aggcccgtga agatatggct
     2101 gcccttgaga aggattatga ggaggttggt gtggattctg ttgaaggaga gggtgaggaa
     2161 gaaggagagg aatactaa
//
