LOCUS       sequence-113679-         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombination-Induced Tag Exchange (RITE) Cassette, T7 to an HA 
            epitope tag, Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Verzijlbergen KF, Menendez-Benito V, van Welsem T, van Deventer SJ, 
            Lindstrom DL, Ovaa H, Neefjes J, Gottschling DE, van Leeuwen F
  TITLE     Recombination-induced tag exchange to track old and new proteins.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Jan 5;107(1):64-8. doi: 
            10.1073/pnas.0911164107. Epub 2009 Dec 14.
  PUBMED    20018668
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0911164107"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2010-01-5- 5"; volume: "107"; issue: "1"; pages: "64-8"
COMMENT     Sequence Label: pTW081
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        8..26
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    33..89
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             complement(107..133)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(142..175)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        270..613
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 agctcggaat taaccctcac taaagggaac aaaagcttgc atgcctgcag gtcgactcta
       61 gaggatcccc gggtaccgag ctcgaattcg cggccgctta tgtacacgca tagtcaggaa
      121 catcgtatgg gtagtcgact gataacttcg tatagcatac attatacgaa gttattctag
      181 actgtgcggt atttcacacc gcatagggtt aattaaggcg cgccagatct gtttagcttg
      241 cctcgtcccc gccgggtcac ccggccagcg acatggaggc ccagaatacc ctccttgaca
      301 gtcttgacgt gcgcagctca ggggcatgat gtgactgtcg cccgtacatt tagcccatac
      361 atccccatgt ataatcattt gcatccatac attttgatgg ccgcacggcg cgaagcaaaa
      421 attacggctc ctcgctgcgg acctgcgagc agggaaacgc tcccctcaca gacgcgttga
      481 attgtcccca cgccgcgccc ctgtagagaa atataaaagg ttaggatttg ccactgaggt
      541 tcttctttca tatacttcct tttaaaatct tgctaggata cagttctcac atcacatccg
      601 aacataaaca accatgggta aaaagcctga actcaccgcg acgtctgtcg agaagtttct
      661 gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg
      721 tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga
      781 tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc
      841 ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc
      901 acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt
      961 cgcggaggcc atgg
//
