LOCUS       sequence-113683-        2193 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  APEX2 fused to the C-terminus human vimentin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, 
            Ting AY
  TITLE     Directed evolution of APEX2 for electron microscopy and proximity 
            labeling.
  JOURNAL   Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179.
  PUBMED    25419960
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179."
COMMENT     Sequence Label: Vimentin - APEX2 in pECFP
FEATURES             Location/Qualifiers
     source          1..2193
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2167..2190
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgtccacca ggtccgtgtc ctcgtcctcc taccgcagga tgttcggcgg cccgggcacc
       61 gcgagccggc cgagctccag ccggagctac gtgactacgt ccacccgcac ctacagcctg
      121 ggcagcgcgc tgcgccccag caccagccgc agcctctacg cctcgtcccc gggcggcgtg
      181 tatgccacgc gctcctctgc cgtgcgcctg cggagcagcg tgcccggggt gcggctcctg
      241 caggactcgg tggacttctc gctggccgac gccatcaaca ccgagttcaa gaacacccgc
      301 accaacgaga aggtggagct gcaggagctg aatgaccgct tcgccaacta catcgacaag
      361 gtgcgcttcc tggagcagca gaataagatc ctgctggccg agctcgagca gctcaagggc
      421 caaggcaagt cgcgcctggg ggacctctac gaggaggaga tgcgggagct gcgccggcag
      481 gtggaccagc taaccaacga caaagcccgc gtcgaggtgg agcgcgacaa cctggccgag
      541 gacatcatgc gcctccggga gaaattgcag gaggagatgc ttcagagaga ggaagccgaa
      601 aacaccctgc aatctttcag acaggatgtt gacaatgcgt ctctggcacg tcttgacctt
      661 gaacgcaaag tggaatcttt gcaagaagag attgcctttt tgaagaaact ccacgaagag
      721 gaaatccagg agctgcaggc tcagattcag gaacagcatg tccaaatcga tgtggatgtt
      781 tccaagcctg acctcacggc tgccctgcgt gacgtacgtc agcaatatga aagtgtggct
      841 gccaagaacc tgcaggaggc agaagaatgg tacaaatcca agtttgctga cctctctgag
      901 gctgccaacc ggaacaatga cgccctgcgc caggcaaagc aggagtccac tgagtaccgg
      961 agacaggtgc agtccctcac ctgtgaagtg gatgccctta aaggaaccaa tgagtccctg
     1021 gaacgccaga tgcgtgaaat ggaagagaac tttgccgttg aagctgctaa ctaccaagac
     1081 actattggcc gcctgcagga tgagattcag aatatgaagg aggaaatggc tcgtcacctt
     1141 cgtgaatacc aagacctgct caatgttaag atggcccttg acattgagat tgccacctac
     1201 aggaagctgc tggaaggcga ggagagcagg atttctctgc ctcttccaaa cttttcctcc
     1261 ctgaacctga gggaaactaa tctggattca ctccctctgg ttgataccca ctcaaaaagg
     1321 acacttctga ttaagacggt tgaaactaga gatggacagg ttatcaacga aacttctcag
     1381 catcacgatg accttgaagg ggatccaccg gtcgccaccg ggaaatcata cccaacagtg
     1441 tccgcagact accaggatgc cgtggagaaa gccaagaaga agctgagagg gtttatcgca
     1501 gaaaagaggt gcgcacctct gatgctgaga ctggctttcc acagcgcagg cacctttgac
     1561 aagggaacca aaacaggcgg accctttgga acaatcaagc accctgctga actggcacat
     1621 agtgccaaca atgggctgga catcgccgtg cggctgctgg aacctctgaa agcagagttc
     1681 ccaattctgt cctacgccga tttttatcag ctggcaggag tggtcgctgt ggaggtcact
     1741 gggggcccca aggtgccttt ccacccagga cgggaggaca aaccagaacc acctccagag
     1801 gggcgcctgc cagatccgac aaagggctcc gaccatctgc gagatgtgtt tgggaaagct
     1861 atgggcctga ctgaccagga tatcgtcgca ctgtctggag ggcacaccat tggcgccgct
     1921 cataaggaaa ggtcaggctt cgagggaccc tggacaagca accccctgat tttcgacaat
     1981 tcttacttta ctgaactgct gagtggagag aaggaagggc tgctgcagct gcccagcgac
     2041 aaagccctgc tgtccgatcc cgtgttcaga cctctggtcg ataagtatgc agccgacgag
     2101 gatgcttttt tcgcagatta cgcagaagca catcagaagc tgtcagaact gggatttgcc
     2161 gacgccgatt ataaggacga tgatgacaaa taa
//
