LOCUS       sequence-113684-        1061 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 
            epitope tag, invariable V5 tag, Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Terweij M, van Welsem T, van Deventer S, Verzijlbergen KF, 
            Menendez-Benito V, Ontoso D, San-Segundo P, Neefjes J, van Leeuwen F
  TITLE     Recombination-induced tag exchange (RITE) cassette series to monitor
            protein dynamics in Saccharomyces cerevisiae.
  JOURNAL   G3 (Bethesda). 2013 Aug 7;3(8):1261-72. doi: 10.1534/g3.113.006213.
  PUBMED    23708297
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.113"; 
            journalName: "G3 (Bethesda)"; date: "2013-08-7- 7"; volume: "3"; 
            issue: "8"; pages: "1261-72"
COMMENT     Sequence Label: pKV006
FEATURES             Location/Qualifiers
     source          1..1061
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             62..103
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    110..143
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             152..178
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      199..386
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(254..278)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     terminator      complement(505..702)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 gggcgaattg ggcccaatgc attggcgccg cggccgcggt ggatctggtg gatctgctag
       61 cggtaagcct atccctaacc ctctcctcgg tctcgattct acgagcgcta taacttcgta
      121 taatgtatgc tatacgaagt tatcaggtac ctacccatac gatgttcctg actatgcgac
      181 tagttaactt ctaaataagc gaatttctta tgatttatga tttttattat taaataagtt
      241 ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc
      301 ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata gtatgaggtc
      361 gctcttattg accacacctc taccggcaga tccgctaggg ataacagggt aaggatccag
      421 attgtactga gagtgcacca taatcatcga tgaattcgag ctcgttttcg acactggatg
      481 gcggcgttag tatcgaatcg acagcagtat agcgaccagc attcacatac gattgacgca
      541 tgatattact ttctgcgcac ttaacttcgc atctgggcag atgatgtcga ggcgaaaaaa
      601 aatataaatc acgctaacat ttgattaaaa tagaacaact acaatataaa aaaactatac
      661 aaatgacaag ttcttgaaaa caagaatctt tttattgtca gtactgatta ttcctttgcc
      721 ctcggacgag tgctggggcg tcggtttcca ctatcggcga gtacttctac acagccatcg
      781 gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat
      841 cggacgattg cgtcgcatcg accctgcgcc caagctgcat catcgaaatt gccgtcaacc
      901 aagctctgat agagttggtc aagaccaatg cggagcatat acgcccggag ccgcggcgat
      961 cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct gctgctccat acaagccaac
     1021 cacggcctcc agaagaagat gttggcgacc tcgtattggg a
//
