LOCUS       sequence-113686-         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter construct under the seabream IL6 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Castellana B, Marin-Juez R, Planas JV
  TITLE     Transcriptional regulation of the gilthead seabream (Sparus aurata) 
            interleukin-6 gene promoter.
  JOURNAL   Fish Shellfish Immunol. 2013 Jul;35(1):71-8. doi: 
            10.1016/j.fsi.2013.04.012. Epub 2013 Apr 18.
  PUBMED    23602849
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.fsi.2013.04.012"; journalName: "Fish Shellfish Immunol"; 
            date: "2013-07"; volume: "35"; issue: "1"; pages: "71-8"
COMMENT     Sequence Label: pGL3-sbIL6P
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     69..87
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     regulatory      150..159
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     189..208
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        189..207
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      238..247
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      242..251
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gatgtccacc tcgatatgtg catctgtaaa agcaattgtt ccaggaacca gggcgtatct
       61 cttcatagcc ttatgcagtt gctctccagc ggttccatct tccagcggat agaatggcgc
      121 cgggcctttc tttatgtttt tggcgtcttc catggtggct ttaccaacag taccggaatg
      181 ccaagcttta atacgactca ctatagggcg aattgggccc gacgtcgcat gctcccggcc
      241 gccatggcgg ccgcgggaat tcgattctgc tgctgctgca agcacttgtt gctctgatgc
      301 tgagggccgt cagggtcgga gggagctgtc gtatctttac gatgggctgt cattttatat
      361 tcttcagacc aggagggatt tccctccctg caacaggcat gaagtagaag tttacgtcaa
      421 gcatccggtt ggctgtggct gatgatggct tcactgatgg aatgtacatg tactctagta
      481 ctttgaggta cttgtacttt actgaagtat tttcattttc tcctatgttt tgcttccact
      541 ctacaaaaac atcctttaga tttcccacat tcatttgaga acctaagtta cgagttactt
      601 ttcagaaggt ggcacatctt tatattcatt tatcttgaaa caaaataaca gaaaaacaga
      661 aaatcacttc gactgatcgg ccgaccctca gtttaaatat acacgtgtga taaatgtgtt
      721 atttactgtc aatttggtac ctttcgatac accttcatct gaaaaccttt actttgaagg
      781 ggcactgaac tattgtttac aatgtaacga gctcataaaa caaactcaaa catttgaatc
      841 ttgttgtcag aacaccagag cggtggc
//
