LOCUS       sequence-113687-        1030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombination-Induced Tag Exchange (RITE) Cassette, T7 to an 
            HA-6xHIS epitope tag, Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Terweij M, van Welsem T, van Deventer S, Verzijlbergen KF, 
            Menendez-Benito V, Ontoso D, San-Segundo P, Neefjes J, van Leeuwen F
  TITLE     Recombination-induced tag exchange (RITE) cassette series to monitor
            protein dynamics in Saccharomyces cerevisiae.
  JOURNAL   G3 (Bethesda). 2013 Aug 7;3(8):1261-72. doi: 10.1534/g3.113.006213.
  PUBMED    23708297
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.113"; 
            journalName: "G3 (Bethesda)"; date: "2013-08-7- 7"; volume: "3"; 
            issue: "8"; pages: "1261-72"
COMMENT     Sequence Label: pTW094
FEATURES             Location/Qualifiers
     source          1..1030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    56..89
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             98..130
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     terminator      151..338
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(206..230)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     terminator      complement(457..654)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 gggcgaattg ggcccaatgc attggcgccg cggccgcggt ggatctggtg gatctataac
       61 ttcgtataat gtatgctata cgaagttatc aggtaccatg gcaagcatga ctggtggaca
      121 gcaaatgggt actagttaac ttctaaataa gcgaatttct tatgatttat gatttttatt
      181 attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta
      241 aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta
      301 tagtatgagg tcgctcttat tgaccacacc tctaccggca gatccgctag ggataacagg
      361 gtaaggatcc gcattgtact gagagtgcac cataatcatc gatgaattcg agctcgtttt
      421 cgacactgga tggcggcgtt agtatcgaat cgacagcagt atagcgacca gcattcacat
      481 acgattgacg catgatatta ctttctgcgc acttaacttc gcatctgggc agatgatgtc
      541 gaggcgaaaa aaaatataaa tcacgctaac atttgattaa aatagaacaa ctacaatata
      601 aaaaaactat acaaatgaca agttcttgaa aacaagaatc tttttattgt cagtactgat
      661 tattcctttg ccctcggacg agtgctgggg cgtcggtttc cactatcggc gagtacttct
      721 acacagccat cggtccagac ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc
      781 ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa
      841 ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa tgcggagcat atacgcccgg
      901 agccgcggcg atcctgcaag ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc
      961 atacaagcca accacggcct ccagaagaag atgttggcga cctcgtattg ggaatccccg
     1021 aacatcgccc
//
