LOCUS       sequence-11369-e        4619 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L
  TITLE     The Q system: a repressible binary system for transgene expression, 
            lineage tracing, and mosaic analysis.
  JOURNAL   Cell. 2010 Apr 30. 141(3):536-48.
  PUBMED    20434990
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 30. 141(3):536-48."
COMMENT     Sequence Label: pQUAS-luc2
FEATURES             Location/Qualifiers
     source          1..4619
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        186..424
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     371..392
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     regulatory      471..480
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             477..2129
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2173..2294
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2184..2203)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2238..2257
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2542..2559)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2713..3301)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2793..2812)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3501..4292,4293..4361))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4466..4514
                     /note="synthetic polyadenylation signal"
     misc_feature    4528..4619
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4568..4587
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tccgggtaat cgcttatcct
       61 cggataaaca attatcctca cgggtaatcg cttatccgct cgggtaatcg cttatcctcg
      121 ggtaatcgct tatccttaag cttgatatcg aattcctgca gcccgagcgg agactctagc
      181 gagcgccgga gtataaatag aggcgcttcg tctacggagc gacaattcaa ttcaaacaag
      241 caaagtgaac acgtcgctaa gcgaaagcta agcaaataaa caagcgcagc tgaacaagct
      301 aaacaatctg cagtaaagtg caagttaaag tgaatcaatt aaaagtaacc agcaaccaag
      361 taaatcaact gcaactactg aaatctgcca agaagtaatt attgaataca agaagagaac
      421 tctgaatagg gaattgggaa ttcgttaaca gatcggggga tccgatatcc gccaccatgg
      481 aagatgccaa aaacattaag aagggcccag cgccattcta cccactcgaa gacgggaccg
      541 ccggcgagca gctgcacaaa gccatgaagc gctacgccct ggtgcccggc accatcgcct
      601 ttaccgacgc acatatcgag gtggacatta cctacgccga gtacttcgag atgagcgttc
      661 ggctggcaga agctatgaag cgctatgggc tgaatacaaa ccatcggatc gtggtgtgca
      721 gcgagaatag cttgcagttc ttcatgcccg tgttgggtgc cctgttcatc ggtgtggctg
      781 tggccccagc taacgacatc tacaacgagc gcgagctgct gaacagcatg ggcatcagcc
      841 agcccaccgt cgtattcgtg agcaagaaag ggctgcaaaa gatcctcaac gtgcaaaaga
      901 agctaccgat catacaaaag atcatcatca tggatagcaa gaccgactac cagggcttcc
      961 aaagcatgta caccttcgtg acttcccatt tgccacccgg cttcaacgag tacgacttcg
     1021 tgcccgagag cttcgaccgg gacaaaacca tcgccctgat catgaacagt agtggcagta
     1081 ccggattgcc caagggcgta gccctaccgc accgcaccgc ttgtgtccga ttcagtcatg
     1141 cccgcgaccc catcttcggc aaccagatca tccccgacac cgctatcctc agcgtggtgc
     1201 catttcacca cggcttcggc atgttcacca cgctgggcta cttgatctgc ggctttcggg
     1261 tcgtgctcat gtaccgcttc gaggaggagc tattcttgcg cagcttgcaa gactataaga
     1321 ttcaatctgc cctgctggtg cccacactat ttagcttctt cgctaagagc actctcatcg
     1381 acaagtacga cctaagcaac ttgcacgaga tcgccagcgg cggggcgccg ctcagcaagg
     1441 aggtaggtga ggccgtggcc aaacgcttcc acctaccagg catccgccag ggctacggcc
     1501 tgacagaaac aaccagcgcc attctgatca cccccgaagg ggacgacaag cctggcgcag
     1561 taggcaaggt ggtgcccttc ttcgaggcta aggtggtgga cttggacacc ggtaagacac
     1621 tgggtgtgaa ccagcgcggc gagctgtgcg tccgtggccc catgatcatg agcggctacg
     1681 ttaacaaccc cgaggctaca aacgctctca tcgacaagga cggctggctg cacagcggcg
     1741 acatcgccta ctgggacgag gacgagcact tcttcatcgt ggaccggctg aagagcctga
     1801 tcaaatacaa gggctaccag gtagccccag ccgaactgga gagcatcctg ctgcaacacc
     1861 ccaacatctt cgacgccggg gtcgccggcc tgcccgacga cgatgccggc gagctgcccg
     1921 ccgcagtcgt cgtgctggaa cacggtaaaa ccatgaccga gaaggagatc gtggactatg
     1981 tggccagcca ggttacaacc gccaagaagc tgcgcggtgg tgttgtgttc gtggacgagg
     2041 tgcctaaagg actgaccggc aagttggacg cccgcaagat ccgcgagatt ctcattaagg
     2101 ccaagaaggg cggcaagatc gccgtgtaat aattctagag tcggggcggc cggccgcttc
     2161 gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     2221 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     2281 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     2341 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc
     2401 cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
     2461 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
     2521 ccggcagcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     2581 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     2641 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2701 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2761 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2821 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2881 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2941 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     3001 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     3061 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     3121 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     3181 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     3241 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     3301 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     3361 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     3421 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gcggccgcaa
     3481 atgctaaacc actgcagtgg ttaccagtgc ttgatcagtg aggcaccgat ctcagcgatc
     3541 tgcctatttc gttcgtccat agtggcctga ctccccgtcg tgtagatcac tacgattcgt
     3601 gagggcttac catcaggccc cagcgcagca atgatgccgc gagagccgcg ttcaccggcc
     3661 cccgatttgt cagcaatgaa ccagccagca gggagggccg agcgaagaag tggtcctgct
     3721 actttgtccg cctccatcca gtctatgagc tgctgtcgtg atgctagagt aagaagttcg
     3781 ccagtgagta gtttccgaag agttgtggcc attgctactg gcatcgtggt atcacgctcg
     3841 tcgttcggta tggcttcgtt caactctggt tcccagcggt caagccgggt cacatgatca
     3901 cccatattat gaagaaatgc agtcagctcc ttagggcctc cgatcgttgt cagaagtaag
     3961 ttggccgcgg tgttgtcgct catggtaatg gcagcactac acaattctct taccgtcatg
     4021 ccatccgtaa gatgcttttc cgtgaccggc gagtactcaa ccaagtcgtt ttgtgagtag
     4081 tgtatacggc gaccaagctg ctcttgcccg gcgtctatac gggacaacac cgcgccacat
     4141 agcagtactt tgaaagtgct catcatcggg aatcgttctt cggggcggaa agactcaagg
     4201 atcttgccgc tattgagatc cagttcgata tagcccactc ttgcacccag ttgatcttca
     4261 gcatctttta ctttcaccag cgtttcgggg tgtgcaaaaa caggcaagca aaatgccgca
     4321 aagaagggaa tgagtgcgac acgaaaatgt tggatgctca tactcgtcct ttttcaatat
     4381 tattgaagca tttatcaggg ttactagtac gtctctcaag gataagtaag taatattaag
     4441 gtacgggagg tattggacag gccgcaataa aatatcttta ttttcattac atctgtgtgt
     4501 tggttttttg tgtgaatcga tagtactaac atacgctctc catcaaaaca aaacgaaaca
     4561 aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa catttctct
//
