LOCUS       sequence-113700-        1132 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombination-Induced Tag Exchange (RITE) Cassette, T7 to an 
            HA-6xHIS epitope tag, Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Terweij M, van Welsem T, van Deventer S, Verzijlbergen KF, 
            Menendez-Benito V, Ontoso D, San-Segundo P, Neefjes J, van Leeuwen F
  TITLE     Recombination-induced tag exchange (RITE) cassette series to monitor
            protein dynamics in Saccharomyces cerevisiae.
  JOURNAL   G3 (Bethesda). 2013 Aug 7;3(8):1261-72. doi: 10.1534/g3.113.006213.
  PUBMED    23708297
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.113"; 
            journalName: "G3 (Bethesda)"; date: "2013-08-7- 7"; volume: "3"; 
            issue: "8"; pages: "1261-72"
COMMENT     Sequence Label: pTW094
FEATURES             Location/Qualifiers
     source          1..1132
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        8..26
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    33..89
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             complement(116..133)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(134..160)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(169..202)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        297..640
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 agctcggaat taaccctcac taaagggaac aaaagcttgc atgcctgcag gtcgactcta
       61 gaggatcccc gggtaccgag ctcgaattcg cggccgctta tgtacattaa ctagtgtgat
      121 ggtgatgatg gtgcgcatag tcaggaacat cgtatgggta agttcctgat aacttcgtat
      181 agcatacatt atacgaagtt attctagact gtgcggtatt tcacaccgca tagggttaat
      241 taaggcgcgc cagatctgtt tagcttgcct cgtccccgcc gggtcacccg gccagcgaca
      301 tggaggccca gaataccctc cttgacagtc ttgacgtgcg cagctcaggg gcatgatgtg
      361 actgtcgccc gtacatttag cccatacatc cccatgtata atcatttgca tccatacatt
      421 ttgatggccg cacggcgcga agcaaaaatt acggctcctc gctgcggacc tgcgagcagg
      481 gaaacgctcc cctcacagac gcgttgaatt gtccccacgc cgcgcccctg tagagaaata
      541 taaaaggtta ggatttgcca ctgaggttct tctttcatat acttcctttt aaaatcttgc
      601 taggatacag ttctcacatc acatccgaac ataaacaacc atgggtaaaa agcctgaact
      661 caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat
      721 gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata
      781 tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca
      841 ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag
      901 cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac
      961 cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga
     1021 tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac
     1081 atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc aa
//
