LOCUS       sequence-11371-e        7917 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7917)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L
  TITLE     The Q system: a repressible binary system for transgene expression, 
            lineage tracing, and mosaic analysis.
  JOURNAL   Cell. 2010 Apr 30. 141(3):536-48.
  PUBMED    20434990
REFERENCE   2  (bases 1 to 7917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 30. 141(3):536-48."
COMMENT     Sequence Label: pCMV-QF
FEATURES             Location/Qualifiers
     source          1..7917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3421..3450
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3466..3483
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3506..3523)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3512..3736
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3782..4210
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3869..3888)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4079..4100
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4219..4239)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4224..4553
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4404..4539
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4466..4485
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4620..5414
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4674..4693)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5284..5303
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5590..5711
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5627..5646)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5681..5700
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5760..5776)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5760..5776)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5773..5795)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5784..5800
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5808..5814,5815..5832,5833..5838))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5853..5874
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5991..6008)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6162..6750)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6242..6261)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6921..7712,7713..7781))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7544..7563
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7782..7886)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttggt accgagctcg gatcccaaca tgccgcctaa acgcaagaca ctcaatgccg
      961 ctgccgaagc caatgcccac gctgatggcc atgctgatgg caatgctgat ggtcatgtcg
     1021 ctaacactgc agcaagcagc aacaacgccc gttttgcgga cttgaccaac attgacacac
     1081 ccggcctcgg ccctaccacg acgacgttac ttgtcgagcc cgctcgttcg aaacgccaga
     1141 gagtctcgag ggcctgtgat cagtgtcgag ctgcacgtga aaagtgtgat ggaatccagc
     1201 cggcttgctt cccctgtgtg tcgcagggcc ggtcgtgtac ctaccaggcc agtcccaaga
     1261 agcgaggagt ccagacgggc tacatccgca ctctcgaact ggctctggct tggatgttcg
     1321 agaacgttgc ccgcagcgag gacgccctcc acaatctttt ggtccgtgat gctggccagg
     1381 gcagcgctct cctggtcggc aaagactcgc ctgctgcaga acgcctgcat gcaagatggg
     1441 cgacgagtcg agtcaacaaa agcatcaccc gtcttctctc aggtcaggcc gcacaagatc
     1501 catctgaaga cggccaatcc ccgtccgaag acataaatgt ccaagatgcc ggggcaaaga
     1561 catccgactt ccctcatgcg cctcacttga ctttctcggc gcctaagtct agtacagctg
     1621 agacacgcac tctaccaggc ccggtccgac cacctatttc ggcaaacacc ctggaaaaca
     1681 accttcagcc agatggtacc gggataggga agctaccacc caatcattgg cgcctgctgg
     1741 atatctactt ttcctacacg cattcttggc tccctatcct cgagaagaaa gacatgtacc
     1801 aagcattgta ccagtactct gaacaaggct cgttgcttcc ctctgcgaat gtcgagtctg
     1861 gcgttcatgc cgagctctgg agcgcgctcg ccctggcgtc cttccaggct gctgctactg
     1921 ctgcatcgag tgctacgggt ccagcttcag ctgctcatgg ccatgacaat gccatcaatc
     1981 cttcacctgc agacatatct gacacagccc gaaagctcat acctttggaa agcgggccgt
     2041 tccaggttca gcactgcaga gcgttgctgc ttctttgtct cgtaagcctt gggcgggatg
     2101 attgggagtc tgcttggttg ctggttggct ttgcggtccg cgtcctactt gttgttcgca
     2161 cccagttgcc tcctgatgat gaccggccac gaccaagaat gcgtgcgctg ctcgtcgcgt
     2221 gcttcatcgt ggataccatt gtgtctatga gacacaacgt gccggcccat ctcaagccag
     2281 acgacattgc ggatctgccg ttacctgaag acggtcaaga tcaatgggag ccgtggacac
     2341 catgtgaggg cttaggcggt gaacacacca tgctgcaaat gttgaggaac ccggcatacc
     2401 ctttaagcac attcaaccac ctatatggcg tgaccaagct ggttgctttg gagcttctgc
     2461 caagaatacg aacatcttca cagaacgctc ccttggagtt caggtcgcgg ttgcagcagg
     2521 taatcggcca caattctccc ttcagcgtct ttgtcctttc ccaggataca gcatcggctt
     2581 ttgtgcctac tgcatacctt acccgtaccg tttatttatg ggcagctgcc ttttctgagc
     2641 ctctcaacga acactactcg catcttctga tcgaaactct tgatcagtat cagaagcggt
     2701 ttggtacata tgcaatccca cctctgatcc cttctcttct agactccctt cttgctttaa
     2761 agaaacaatc acattcttca gagcggcatc gaaggcactt ggaagagctt ttccccgcct
     2821 actcctccat ttggcctcgg ggaggccgac acagcaatac tggcctccaa cccatacgac
     2881 aacttgagct tccgccgact gcgactgcca ctgcaagtat catgccccat gtcatggaac
     2941 agcccctgtc aacgtcaata aatccggtca atgatcggtt taatggaata ccgaatccta
     3001 ccccctacaa tagcgatgcg gccctagacg caattactca aaccaatgac tacggatcag
     3061 tcaataccca tggcattctc agtacatatc caccacctgc cacccacttg aacgaggcct
     3121 cggtggccct tgcgcctgga ggtgctcctc ccagaccacc cccgccatac gttgatagta
     3181 caacgaacca tcctccttac catagtaacc tcgtccccat ggccaatttc ggatattcga
     3241 ctgtagacta tgatgcgatg gtggatgatc tggcatcgat cgagtacacg gatgcggttg
     3301 acgttgatcc gcagttcatg acgaacctcg ggtttgtccc agggtgtaat tttagcgata
     3361 tcaacacgta tgagcaatag ttggctattc gagcggccgc tcgagtctag agggcccttc
     3421 gaacaaaaac tcatctcaga agaggatctg aatatgcata ccggtcatca tcaccatcac
     3481 cattgagttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     3541 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     3601 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     3661 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     3721 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     3781 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     3841 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     3901 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     3961 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     4021 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     4081 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     4141 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     4201 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     4261 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     4321 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     4381 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     4441 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     4501 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     4561 cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca
     4621 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     4681 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     4741 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     4801 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     4861 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     4921 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     4981 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     5041 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     5101 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     5161 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     5221 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     5281 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     5341 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     5401 acgagttctt ctgagcggga ctctggggtt cgcgaaatga ccgaccaagc gacgcccaac
     5461 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     5521 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     5581 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     5641 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     5701 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     5761 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     5821 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     5881 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     5941 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     6001 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     6061 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     6121 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     6181 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     6241 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     6301 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     6361 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     6421 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     6481 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     6541 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     6601 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     6661 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     6721 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     6781 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     6841 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     6901 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     6961 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     7021 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     7081 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     7141 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     7201 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     7261 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     7321 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     7381 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     7441 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     7501 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     7561 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     7621 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     7681 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     7741 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     7801 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     7861 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
