LOCUS       sequence-113721-         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Kwon Y, Hofmann T, Montell C
  TITLE     Integration of phosphoinositide- and calmodulin-mediated regulation 
            of TRPC6.
  JOURNAL   Mol Cell. 2007 Feb 23. 25(4):491-503.
  PUBMED    17317623
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2007 Feb 23. 25(4):491-503."
COMMENT     Sequence Label: pcDNA3-AKT-PH-GFP
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      541..550
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(592..613)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(853..872)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttgcg gccgcgaatt cgccaccatg agcgacgtgg ctattgtgaa ggagggttgg
      121 ctgcacaaac gaggggagta catcaagacc tggcggccac gctacttcct cctcaagaat
      181 gatggcacct tcattggcta caaggagcgg ccgcaggatg tggaccaacg tgaggctccc
      241 ctcaacaact tctctgtggc gcagtgccag ctgatgaaga cggagcggcc ccggcccaac
      301 accttcatca tccgctgcct gcagtggacc actgtcatcg aacgcacctt ccatgtggag
      361 actcctgagg agcgggagga gtggacaacc gccatccaga ctgtggctga cggcctcaag
      421 aagcaggagg aggaggagat ggacttccgg tcgggctcac ccagtgacaa ctcaggggct
      481 gaagagatgg aggtgtccct ggccaagccc aagcaccgcg tgaccatgaa cggatccatc
      541 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
      601 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
      661 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
      721 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
      781 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
      841 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
      901 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg ca
//
