LOCUS       sequence-113723-         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express Oct4, Klf4, Sox2, c-Myc and hairpin RNA p53. A single 
            plasmid reprogramming system using a mini-intronic plasmid (MIP)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Diecke S, Lu J, Lee J, Termglinchan V, Kooreman NG, Burridge PW, 
            Ebert AD, Churko JM, Sharma A, Kay MA, Wu JC
  TITLE     Novel codon-optimized mini-intronic plasmid for efficient, 
            inexpensive, and xeno-free induction of pluripotency.
  JOURNAL   Sci Rep. 2015 Jan 28;5:8081. doi: 10.1038/srep08081.
  PUBMED    25628230
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-01-28"; volume: "5:8081. doi"; pages: " 
            10.1038/srep08081"
COMMENT     Sequence Label: MIP 247 CoMiP 4in1 with shRNA p53
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    105..329
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             447..458
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gccttgtttg ggccgactcc agtggtaatc tacttcaaga gagtagatta ccactggagt
       61 cttttttgga attcctgcag cccgggacta gagatatcag atctccatag agcccaccgc
      121 atccccagca tgcctgctat tgtcttccca atcctccccc ttgctgtcct gccccacccc
      181 accccccaga atagaatgac acctactcag acaatgcgat gcaatttcct cattttatta
      241 ggaaaggaca gtgggagtgg caccttccag ggtcaaggaa ggcacggggg aggggcaaac
      301 aacagatggc tggcaactag aaggcacagc tcgagtccag gcggggaggc ggcccaaagg
      361 gagatccgac tcgtctgagg gcgaaggcga agacgcggaa gaggccgcag agccggcagc
      421 aggccgcggg aaggaaggtc cgctggattg agggccgaag ggacgtagca gaaggacgtc
      481 ccgcgcagaa tccaggtggc aacacaggcg agcagccatg gaaaggacgt cagcttcccc
      541 gacaacacca cggaattgtc agtgcccaac agccgagccc ctgtccagca gcgggcaagg
      601 caggcggcga tgagttccgc cgtggcaata gggaggggga aagcgaaagt cccggaaagg
      661 agctgacagg tggtggcaat gccccaacca gtgggggttg cgtcagcaaa cacagtgcac
      721 accacgccac gttgcctgac aac
//
