LOCUS       sequence-113728-         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Naviaux RK, Costanzi E, Haas M, Verma IM
  TITLE     The pCL vector system: rapid production of helper-free, high-titer, 
            recombinant retroviruses.
  JOURNAL   J Virol. 1996 Aug . 70(8):5701-5.
  PUBMED    8764092
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            1996 Aug . 70(8):5701-5."
COMMENT     Sequence Label: pCL-Eco
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             328..744
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     674..696
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     712..728
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 ctatataagc agagctcaat aaaagagccc acaacccctc actcggcgcg ccagtcctcc
       61 gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga
      121 cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg
      181 gggtctttca tttgggggct cgtccgggat cgggagaccc ctgcccaggg accaccgacc
      241 caccaccggg aggtaagctg gccaagcttg ggcatcgttc tgtgttgtct ctgtctgact
      301 gtgtttctgt atttgtctga aaatatgggc cagactgtta ccactccctt aagtttgacc
      361 ttaggtcact ggaaagatgt cgagcggatc gctcacaacc agtcggtaga tgtcaagaag
      421 agacgttggg ttaccttctg ctctgcagaa tggccaacct ttaacgtcgg atggccgcga
      481 gacggcacct ttaaccgaga cctcatcacc caggttaaga tcaaggtctt ttcacctggc
      541 ccgcatggac acccagacca ggtcccctac atcgtgacct gggaagcctt ggcttttgac
      601 ccccctccct gggtcaagcc ctttgtacac cctaagcctc cgcctcctct tcctccatcc
      661 gccccgtctc tcccccttga acctcctcgt tcgaccccgc ctcgatcctc cctttatcca
      721 gccctcactc cttctctagg cgccaaacct aaacctcaag ttctttctga cagtgggggg
      781 ccgctcatcg acctacttac agaagacccc ccgccttata gggacccaag accaccccct
      841 tccgacaggg acggaaatgg tggagaagcg acccctgcgg gagaggcacc ggacccctcc
      901 ccaatggcat ctcgcctacg tgggagacgg gagccccc
//
