LOCUS       sequence-11373-e        9229 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9229)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L
  TITLE     The Q system: a repressible binary system for transgene expression, 
            lineage tracing, and mosaic analysis.
  JOURNAL   Cell. 2010 Apr 30. 141(3):536-48.
  PUBMED    20434990
REFERENCE   2  (bases 1 to 9229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 30. 141(3):536-48."
COMMENT     Sequence Label: pAC-QS
FEATURES             Location/Qualifiers
     source          1..9229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2187..2209
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2206..2222
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2277..4790
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     primer_bind     complement(8835..8852)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8835..8851)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8844..8866)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9060..9079)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9179..9201
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgaattctat attctaaaaa cacaaatgat acttctaaaa aaaaatcatg
     2281 aatggcatca actctgaatc aaatctttgc agatgcacct acttctcatt tccactgtca
     2341 catcattttt ccagatctcg ctgcctgtta tgtggcccac aaaccaagac acgttttatg
     2401 gccattaaag ctggctgatc gtcgccaaac accaaataca taatgaatat gtacacattc
     2461 gagaaagaag cgatcaaaga agcgtcttcg ggcggagtag gagaatgcgg aggagaagga
     2521 gaacgagctg atctagtatc tctccacaat ccaatgccaa ctgaccaact ggccatattc
     2581 ggagcaattt gaagccaatt tccatcgcct ggcgatcgct ccattcttgg ctatatgttt
     2641 ttcaccgtta cccggggcca ttttcaaaga ctcgtcggca agataagatt gtgtcactcg
     2701 ctgtctctct tcatttgtcg aagaatgctg aggaatttcg cgatgacgtc ggcgagtatt
     2761 ttgaagaatg agaataattt gtatttatac gaaaatcagt tagtggaatt ttctacaaaa
     2821 acatgttatc tatagataat tttgttgcaa aatatgttga ctatgacaaa gattgtatgt
     2881 atataccttt aatgtattct cattttctta tgtatttata atggcaatga tgatactgat
     2941 gatattttaa gatgatgcca gaccaaaagg cttgaatttc tgcgtctttt gccgaacgca
     3001 gtgcatgtgc aattgttgtt ttttggaata ttcaattttc ggactgtccg ctttgatttc
     3061 agtttcttgg cttattcaaa aagcaaagta aagccaaaaa agcgagatgg caataccaaa
     3121 tgcggcaaaa cggtagtgga aggaaagggg tgcggggcag cggaaggaag ggtggggcgg
     3181 ggcgtggcgg ggtctgtggc tgggcgcgac gtcaccgacg ttggagccac tcctttgacc
     3241 atgtgtgcgt gtgtgtatta ttcgtgtctc gccactcgcc ggttgttttt ttctttttat
     3301 gctgcgctct ctctagcgcc atctcgctta cgcatgctca acgcaccgca tgttgccgtt
     3361 tccttttatg cgtcattttg gctcgaaata ggcaattatt taaacaaaga ttagtcaacg
     3421 aaaacgctaa aataaataag tctacaatat ggttacttat tgccatgtgt gtgcagccaa
     3481 cgatagcaac aaaagcaaca acacaggtgg ctttccctct ttcacttttt gtttgcaagc
     3541 cgcgtgcgag caagacggca cgaccggcaa acgcaattac gctgacaaag agcagacgaa
     3601 gttttggcga aaaacatcaa ggcgcctgat acgaatgcat ttgcaataac aattgcgata
     3661 tttaatattg tttatgaagc tgtttgactt caaaacacac aaaaaaaaaa ataaaacaaa
     3721 ttatttgaaa gagaattagg aatcggacgc ttatcgttag ggtaacaaca agaaatgctt
     3781 actgagtcac agcctctgga aaactgccgc aagccagaga gagagagaaa aagagggaga
     3841 gcagcttaga ccgcatgtgc ttgtgtgtga ggcgtctctc tcttcgtctc tgttgcgcaa
     3901 acgcatagac tgcactgaaa aaatcgatta cctatttttt atgaatgaat atttgcacta
     3961 ttactattca aaactattaa gatagcaatc acattcaata gccaaatact ataccacctg
     4021 agcgatgcaa cgaaatgatc aatttgagca aaaatgctgc atatttagga cggcatcatt
     4081 atagaaatgc ttcttgctgt gtacttttct ctcgtctggc agctgtttcg ccgttattgt
     4141 taaaaccggc ttaagttagg tgtgttttct acgactagct agtgaatgcc ctactagaag
     4201 atgtgtgttg cacaaaatgt ccctggaata accaatttga agtgcagata gcagtaaacg
     4261 taagctaata tgaatattat ttaactgtaa tgttttaata tcgctggaca ttactaataa
     4321 acccactata aacacatgta catatgtatg ttttggcata caatgagtag ttggggaaaa
     4381 aatgtgtaaa agcaccgtga ccatcacagc ataaagataa ccagctgaag tatcgaatat
     4441 gagtaacccc caaattgaat cacatgccgc aactgatagg acccatggaa gtacactctt
     4501 catggcgata tacaagacac acacaagcac gaacacccag ttgcggagga aattctccgt
     4561 aaatgaaaac ccaatcggcg aacaattcat acccatatat ggtaaaagtt ttgaacgcga
     4621 cttgagagcg gagagcattg cggctgataa ggttttagcg ctaagcgggc tttataaaac
     4681 gggctgcggg accagttttc atatcactac cgtttgagtt cttgtgctgt gtggatactc
     4741 ctcccgacac aaagccgctc catcagccag cagtcgtcta atccagagac cccggatccg
     4801 atatcactag ttctagaggt acccaacatg aacaccatcc cggcacgcca tgtcggggat
     4861 gtcgccgccc gcgatcctct acctctaccg cacatatcat cctccgtcgc cagcggcatg
     4921 aagcgttcct tcgcaaccat ggccatgctc tacaacgaca ctggcaacag caacgatgtc
     4981 ggtgcccatg ccaggcgacc accacgaacc ctctccaata gtcggagcac ttccgcccac
     5041 agagtacctc taggctcttg gtcggcgccc aattccccgc cccgccgtgc gctgccgcat
     5101 catcccatca ccgccagctt cgatcccgat gcttccattg ttatcgccgg cattcgtggc
     5161 gctggcaagt ctacgctggc catcatggca tctaccgcca tgaagcgcaa gatagtcgac
     5221 ctggaatccg agttccatca tcttactggc ttgtctagtt ccagctacaa gaagacacac
     5281 ggcccggtcg actatgggag gcgccagatc gccatcttgc agaacatctt gaatctgcac
     5341 aggacccgcg ccattctcgt ctgctcttgg ctggagcggg atgtgcaggc catgttgcag
     5401 gatttcagcg tgtctaatcc tgtcatttac gttctgcgcg atgccaaagc tatcgaggcc
     5461 catctgaagg gatacgacaa gtccaaagtt ggcaccctcc ttgatgccac cagtactgtc
     5521 cttcgccgct gcacccgttt cgagttcttc aacgtctccg aagagaacct ggacacccac
     5581 tccgcttcaa catcaccacc tgctgttccg gaccagcggc ataccgcgcc gtatctaacg
     5641 cttaaacgag ccgagcgcca cttcctcaaa tttctctcct tgattctacc caaggggacc
     5701 ataccttttg tcgagtccgc ctttcccctg gcttccgtcc ccgttgaaca gcgccgcttt
     5761 acctacgccc tcgccttgcc tgtatctgcc ttgctcgaca aaggcgtcga tatccaagag
     5821 cttgatgtcg gtgtagacgc aatcgagatc attgtagacg atcttgcaac gagcgaatcc
     5881 ggcccaacga gccccttggg tcttgcgccc caccgagcga gcgagatcag tcgtgttgta
     5941 ggcgaaatca ggagggacac agtgatcccc atcattctgc acgtggtctt tccagaaaga
     6001 gcgctatatg aagaagctct gctcgcgctt tacatgactt acctgaacca tgccttaagg
     6061 cttgcgccag attatcttac ggtcgatctg gggctcgatt ctggcttgct tgggcaacta
     6121 accaccgttc aaggaaccac caaggtcatc ggcaataaac aacttgcaga ggtcaattcg
     6181 ccgcgctggg gggatccatc ttggttacaa gcctatgaaa aggcccagaa tacaggatgc
     6241 gacttggtaa ggttgaccag accggcttca aatccccggg acaacacaga cattcggcag
     6301 ttccacgttg ctgtagaggc cgtcgggggt ccaaggctcc catttattgc ttacaacaca
     6361 ggacgcctag gtcggacatc gatgtgtttt aacgagatcc tgactccagt tacaccagtg
     6421 cctaccaagg aggatgcaat cgggctccgc aatccagccc atcgctatct ccagcctccg
     6481 ctcacggctc tggaagcaac acaggctctc tactcggcat ttgtccacga cccaatgaag
     6541 ctgtatgtct ttggcgcaaa tgtgggatat agcttgtccc cagccatgca caatgccgca
     6601 ctcaaggcct gtggcattcc acaccattac aagccccttt ccacagcaaa catcgggact
     6661 ttgcgcgagg ttatcagcga tccgcagttt gctggagcct cggtcggcct gccgtttaag
     6721 gtggaaatca tcagcctcac acactcgctg agccggcacg cgaaagccat cggagccgtc
     6781 aacaccttga ttccggtacg acaccttacc gcggacggtg gaataccgga cgaggtgtcc
     6841 atgttcaaca atatcagcca agccggcgct gtcagagctc tctacggcga gaacacggat
     6901 tggattggta tccgagcctg ccttcgccgc ggtttatcgc ccgccaatgc cgtgagatca
     6961 acaagcactg gtcttgtcat cggcgctggc ggaatggcta gggcagctgt ctatgccatg
     7021 cttcaactgg gagtcaagaa gattttgatc tttaaccgaa catttgctaa tgccgagaag
     7081 ctggttctac acttcgagaa cctgttggtc agagacgcat tgcctctgtt gagcacaggg
     7141 ccaagatccc acgacaacac ctgttttcac atcattcgat ctcgagacga tccgctccca
     7201 gaaaacttca aaaacccgac catgatcgtt tcctgcatac cgacacacac agtggacaac
     7261 acccctgacc ctgaatttac tgtgcctttg cactggctcg acaaccccac tggcggcatt
     7321 gtactagaac tcgactacaa atgtctcaca tcacccttgc tcgaacaaac acgacgcgag
     7381 gctcacagag gctgggtcgc aatggatgga cttgacctct tgccagaaca agggtttgcc
     7441 caatttgaac tgttcaccgg gcggcgagca cctcgtcgct tgatgaggcg cgaggttttg
     7501 cgagcatacc cagatgatca agcaaaatct cataccgcgc agttacagcc tcgcctcaac
     7561 ggaattgcaa cgcaaatatc ttgagcggcc gcgttaaccg atccgtcgac catgaagatc
     7621 aagatcattg ccccgccaga gcgcaagtac tctgtctgga tcggtggctc catcctggct
     7681 tcgctgtcca ccttccagca gatgtggatc tccaagcagg agtacgacga gtccggcccc
     7741 tccattgtgc accgcaagtg cttctaagaa ggatcgcttg tctgggcaag aggatcagga
     7801 tcgggatggt cttgattctg ctggaggagg aggaggagaa gtcgaggaag cagcagcgaa
     7861 agtgcaagtg cgagtggtgg aagtttggag tgcagcacaa caaaatcaac aacaacacca
     7921 actacaagat gaaaagagcg gaaccacctg cacaccatca tcactatcat catcgttttg
     7981 ggcgcatgtt gtgtggttcc agcgtattaa tataattaat ttattccaca tgagatatga
     8041 tatgatatac tatgtatttt ttgttttttt tttatttgta aacctttaat ataacaagaa
     8101 ctacaaaaaa tgaaaatgag cgaaaatgca tattctgcca ttccacacac acaccaacaa
     8161 cacccaacac acgcacaccc acaagcttac acacacacat tcgcggcatg acaaggacat
     8221 caagataaag aagaacttaa agaagatatt tcccaaagcg caaaaagaac acacacacat
     8281 tgcaaaacac aaacaacaca ctagcgtttt gtacaattcg tcagcaacct tatgtattat
     8341 tttttaatta tgatgtaatt ataaacaaag tgaaacaaaa atatgaaaac aaaaaggaaa
     8401 atcaaatctg tcttctcttt ctcccgctct cctcgctctc tgctgctaac ctcgccctct
     8461 cctctctcat ctttttgtct gtctctcttc cacatttttg ccggccggca aaataataac
     8521 ccacacacac tcacacttgg ctgcagtttc gcgtgcgata ttcacacaca ttcaagcata
     8581 caagcataca tacatatgta tttttttttt atttgtacac ttttctaatt gcatgcgtat
     8641 cgattgataa gtttacgcct gaaaatgtta attaaaatgt gaaaatgcaa ctgaaaaact
     8701 gatgaaatga aacaacaaca agcgaacaat ttgctacatg tgtattgtct aacaaccgtt
     8761 actgcaacgg ttgcttcgga aaagggtgaa gaggaagagg gcacgaggtc gacctgcagg
     8821 catgcaagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     8881 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     8941 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg
     9001 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
     9061 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     9121 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     9181 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcga
//
