LOCUS       sequence-113735-         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  source of KmR FRT cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer 
            RR, Schweizer HP
  TITLE     A Tn7-based broad-range bacterial cloning and expression system.
  JOURNAL   Nat Methods. 2005 Jun;2(6):443-8.
  PUBMED    15908923
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2005-06"; volume: "2(6)"; pages: "443-8"
COMMENT     Sequence Label: pFKM1
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    21..68
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 gagctcgaat tggggatctt gaagtaccta ttccgaagtt cctattctct agaaagtata
       61 ggaacttcag agcgcttttg aagctgatgt gcttaaaaac ttactcaatg gaataattct
      121 agatgggaag tcagcgtaat gctctgccag tgttacaacc aattaaccaa ttctgattag
      181 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca
      241 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg
      301 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt
      361 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa
      421 tccggtgaga atggcaagag cttatgcatt tctttccaga cttgttcaac aggccagcca
      481 ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc
      541 tgagcgagac gaaatacgca attgctgtta aaaggacaat tacaaacagg aatcgaatgc
      601 aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct
      661 tctaatacct ggaatgctgt tttgccgggg atcgcagtgg tgagtaacca tgcatcatca
      721 ggagtacgga t
//
