LOCUS       sequence-113737-         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  source of KmR FRT cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer 
            RR, Schweizer HP
  TITLE     A Tn7-based broad-range bacterial cloning and expression system.
  JOURNAL   Nat Methods. 2005 Jun;2(6):443-8.
  PUBMED    15908923
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2005-06"; volume: "2(6)"; pages: "443-8"
COMMENT     Sequence Label: pFKM1
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(46..93)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(232..251)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 gaggatcccc gggtaccgag ctcgaattag cttcaaaagc gctctgaagt tcctatactt
       61 tctagagaat aggaacttcg gaataggaac ttcaagatcc cctgattccc tttgtcaaca
      121 gcaatggatg ggaaagccac gttgtgtctc aaaatctctg atgttacatt gcacaagata
      181 aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg
      241 ttatgagcca tattcaacgg gaaacgtctt gctccaggcc gcgattaaat tccatcatgg
      301 atgctgattt atatgggtat aaatgggcac gcgataatgt cgggcaatca ggtgcgacaa
      361 tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgagacat ggcaaaggta
      421 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
      481 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
      541 cgatccccgg caaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
      601 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc
      661 cttttaacag caattgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt
      721 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttg
//
