LOCUS       sequence-113753-        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a wild-type sequence of LplA and serves as the negative 
            control for resorufin labeling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Liu DS, Nivon LG, Richter F, Goldman PJ, Deerinck TJ, Yao JZ, 
            Richardson D, Phipps WS, Ye AZ, Ellisman MH, Drennan CL, Baker D, 
            Ting AY
  TITLE     Computational design of a red fluorophore ligase for site-specific 
            protein labeling in living cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Oct 28;111(43):E4551-9. doi: 
            10.1073/pnas.1404736111. Epub 2014 Oct 13.
  PUBMED    25313043
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1404736111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-10-28- 28"; volume: "111"; issue: "43"; pages: "E4551-9"
COMMENT     Sequence Label: pcDNA3-GFP-LpIA(wild-type)
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..23
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5..22
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tagcatttag gtgacactat agaatagggc cctctagagg gcatcgttcc cactgcgatg
       61 ctggttgcca acgatcagat ggcgttgggc gcaatgcgcg ccattaccga gtccgggctg
      121 cgcgttggtg cgctcgagcg gccgccagtg tgatggatat ctgcagaatt cttatctcac
      181 ggcgccggcc atccaggcgc tcagctctct cagctccttc tcctgctcgg ggaagtccac
      241 cagcagggcc tcgcactcct gctgcagcat gtcggctctg tacaggcagc cctgcagtct
      301 gccggccagg gcctccaggg gggcggggtt caggctgtcg gtgaacacct gggctctggt
      361 gatgtggccc ttctccacgt cgaagtgcag ctccacgccg ccccaggtga atctctcgtc
      421 cagcaggtgg ctgaaggcgg gggcctggcc gaagttccac tcccagctgc tctgtctggc
      481 gaaggtctcg gcgaagttgg gcaggtcggg ggtcttgttg gggctgatga tctcggcctc
      541 cactctctcg ccgtagtggg cgaagaaggc ctcggtgatg gcctcgcaca cctgctcgtg
      601 ggtgatgccg ggcagcagct cggtcaggtt ggtcactctg cttctcacgc tggtgatgcc
      661 cttggcggcc agcttcttct tgtcggggtt caggtagttg gccagtctgc tcaggtcggc
      721 gttcagcagc agggtgccgt ggtggaagcc tctgtccttg gtctctctgt aggcgctgcc
      781 gctcaccttt ctgtcgccct ccacggtctt caccaccagg tcgtttctgc cgctggcctc
      841 ggcgctcacg cccagggcgt tcagggcgtt cagcacgatg ctggtgctga tggtcttgtc
      901 gtactcgggc ttgccggcca tgaaggtgaa gcaggtgttg cccaggtcgt ggaacacggc
      961 gccgccgccg ctgcttcttc tggccagtct cacgttgtcc tcctccattc ttctgg
//
