LOCUS       sequence-11376-e        9094 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9094)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L
  TITLE     The Q system: a repressible binary system for transgene expression, 
            lineage tracing, and mosaic analysis.
  JOURNAL   Cell. 2010 Apr 30. 141(3):536-48.
  PUBMED    20434990
REFERENCE   2  (bases 1 to 9094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 30. 141(3):536-48."
COMMENT     Sequence Label: pAC-GAL4
FEATURES             Location/Qualifiers
     source          1..9094
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2187..2209
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2206..2222
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2277..4790
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             4804..7449
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4"
                     /product="GAL4 transcriptional activator"
                     /label=GAL4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS
                     IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA
                     KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL
                     RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW
                     NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI
                     QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY
                     VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL
                     QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     primer_bind     complement(8700..8717)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8700..8716)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8709..8731)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8925..8944)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9044..9066
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgaattctat attctaaaaa cacaaatgat acttctaaaa aaaaatcatg
     2281 aatggcatca actctgaatc aaatctttgc agatgcacct acttctcatt tccactgtca
     2341 catcattttt ccagatctcg ctgcctgtta tgtggcccac aaaccaagac acgttttatg
     2401 gccattaaag ctggctgatc gtcgccaaac accaaataca taatgaatat gtacacattc
     2461 gagaaagaag cgatcaaaga agcgtcttcg ggcggagtag gagaatgcgg aggagaagga
     2521 gaacgagctg atctagtatc tctccacaat ccaatgccaa ctgaccaact ggccatattc
     2581 ggagcaattt gaagccaatt tccatcgcct ggcgatcgct ccattcttgg ctatatgttt
     2641 ttcaccgtta cccggggcca ttttcaaaga ctcgtcggca agataagatt gtgtcactcg
     2701 ctgtctctct tcatttgtcg aagaatgctg aggaatttcg cgatgacgtc ggcgagtatt
     2761 ttgaagaatg agaataattt gtatttatac gaaaatcagt tagtggaatt ttctacaaaa
     2821 acatgttatc tatagataat tttgttgcaa aatatgttga ctatgacaaa gattgtatgt
     2881 atataccttt aatgtattct cattttctta tgtatttata atggcaatga tgatactgat
     2941 gatattttaa gatgatgcca gaccaaaagg cttgaatttc tgcgtctttt gccgaacgca
     3001 gtgcatgtgc aattgttgtt ttttggaata ttcaattttc ggactgtccg ctttgatttc
     3061 agtttcttgg cttattcaaa aagcaaagta aagccaaaaa agcgagatgg caataccaaa
     3121 tgcggcaaaa cggtagtgga aggaaagggg tgcggggcag cggaaggaag ggtggggcgg
     3181 ggcgtggcgg ggtctgtggc tgggcgcgac gtcaccgacg ttggagccac tcctttgacc
     3241 atgtgtgcgt gtgtgtatta ttcgtgtctc gccactcgcc ggttgttttt ttctttttat
     3301 gctgcgctct ctctagcgcc atctcgctta cgcatgctca acgcaccgca tgttgccgtt
     3361 tccttttatg cgtcattttg gctcgaaata ggcaattatt taaacaaaga ttagtcaacg
     3421 aaaacgctaa aataaataag tctacaatat ggttacttat tgccatgtgt gtgcagccaa
     3481 cgatagcaac aaaagcaaca acacaggtgg ctttccctct ttcacttttt gtttgcaagc
     3541 cgcgtgcgag caagacggca cgaccggcaa acgcaattac gctgacaaag agcagacgaa
     3601 gttttggcga aaaacatcaa ggcgcctgat acgaatgcat ttgcaataac aattgcgata
     3661 tttaatattg tttatgaagc tgtttgactt caaaacacac aaaaaaaaaa ataaaacaaa
     3721 ttatttgaaa gagaattagg aatcggacgc ttatcgttag ggtaacaaca agaaatgctt
     3781 actgagtcac agcctctgga aaactgccgc aagccagaga gagagagaaa aagagggaga
     3841 gcagcttaga ccgcatgtgc ttgtgtgtga ggcgtctctc tcttcgtctc tgttgcgcaa
     3901 acgcatagac tgcactgaaa aaatcgatta cctatttttt atgaatgaat atttgcacta
     3961 ttactattca aaactattaa gatagcaatc acattcaata gccaaatact ataccacctg
     4021 agcgatgcaa cgaaatgatc aatttgagca aaaatgctgc atatttagga cggcatcatt
     4081 atagaaatgc ttcttgctgt gtacttttct ctcgtctggc agctgtttcg ccgttattgt
     4141 taaaaccggc ttaagttagg tgtgttttct acgactagct agtgaatgcc ctactagaag
     4201 atgtgtgttg cacaaaatgt ccctggaata accaatttga agtgcagata gcagtaaacg
     4261 taagctaata tgaatattat ttaactgtaa tgttttaata tcgctggaca ttactaataa
     4321 acccactata aacacatgta catatgtatg ttttggcata caatgagtag ttggggaaaa
     4381 aatgtgtaaa agcaccgtga ccatcacagc ataaagataa ccagctgaag tatcgaatat
     4441 gagtaacccc caaattgaat cacatgccgc aactgatagg acccatggaa gtacactctt
     4501 catggcgata tacaagacac acacaagcac gaacacccag ttgcggagga aattctccgt
     4561 aaatgaaaac ccaatcggcg aacaattcat acccatatat ggtaaaagtt ttgaacgcga
     4621 cttgagagcg gagagcattg cggctgataa ggttttagcg ctaagcgggc tttataaaac
     4681 gggctgcggg accagttttc atatcactac cgtttgagtt cttgtgctgt gtggatactc
     4741 ctcccgacac aaagccgctc catcagccag cagtcgtcta atccagagac cccggatccc
     4801 aacatgaagc tactgtcttc tatcgaacaa gcatgcgata tttgccgact taaaaagctc
     4861 aagtgctcca aagaaaaacc gaagtgcgcc aagtgtctga agaacaactg ggagtgtcgc
     4921 tactctccca aaaccaaaag gtctccgctg actagggcac atctgacaga agtggaatca
     4981 aggctagaaa gactggaaca gctatttcta ctgatttttc ctcgagaaga ccttgacatg
     5041 attttgaaaa tggattcttt acaggatata aaagcattgt taacaggatt atttgtacaa
     5101 gataatgtga ataaagatgc cgtcacagat agattggctt cagtggagac tgatatgcct
     5161 ctaacattga gacagcatag aataagtgcg acatcatcat cggaagagag tagtaacaaa
     5221 ggtcaaagac agttgactgt atcgattgac tcggcagctc atcatgataa ctccacaatt
     5281 ccgttggatt ttatgcccag ggatgctctt catggatttg attggtctga agaggatgac
     5341 atgtcggatg gcttgccctt cctgaaaacg gaccccaaca ataatgggtt ctttggcgac
     5401 ggttctctct tatgtattct tcgatctatt ggctttaaac cggaaaatta cacgaactct
     5461 aacgttaaca ggctcccgac catgattacg gatagataca cgttggcttc tagatccaca
     5521 acatcccgtt tacttcaaag ttatctcaat aattttcacc cctactgccc tatcgtgcac
     5581 tcaccgacgc taatgatgtt gtataataac cagattgaaa tcgcgtcgaa ggatcaatgg
     5641 caaatccttt ttaactgcat attagccatt ggagcctggt gtatagaggg ggaatctact
     5701 gatatagatg ttttttacta tcaaaatgct aaatctcatt tgacgagcaa ggtcttcgag
     5761 tcaggttcca taattttggt gacagcccta catcttctgt cgcgatatac acagtggagg
     5821 cagaaaacaa atactagcta taattttcac agcttttcca taagaatggc catatcattg
     5881 ggcttgaata gggacctccc ctcgtccttc agtgatagca gcattctgga acaaagacgc
     5941 cgaatttggt ggtctgtcta ctcttgggag atccaattgt ccctgcttta tggtcgatcc
     6001 atccagcttt ctcagaatac aatctccttc ccttcttctg tcgacgatgt gcagcgtacc
     6061 acaacaggtc ccaccatata tcatggcatc attgaaacag caaggctctt acaagttttc
     6121 acaaaaatct atgaactaga caaaacagta actgcagaaa aaagtcctat atgtgcaaaa
     6181 aaatgcttga tgatttgtaa tgagattgag gaggtttcga gacaggcacc aaagttttta
     6241 caaatggata tttccaccac cgctctaacc aatttgttga aggaacaccc ttggctatcc
     6301 tttacaagat tcgaactgaa gtggaaacag ttgtctctta tcatttatgt attaagagat
     6361 tttttcacta attttaccca gaaaaagtca caactagaac aggatcaaaa tgatcatcaa
     6421 agttatgaag ttaaacgatg ctccatcatg ttaagcgatg cagcacaaag aactgttatg
     6481 tctgtaagta gctatatgga caatcataat gtcaccccat attttgcctg gaattgttct
     6541 tattacttgt tcaatgcagt cctagtaccc ataaagactc tactctcaaa ctcaaaatcg
     6601 aatgctgaga ataacgagac cgcacaatta ttacaacaaa ttaacactgt tctgatgcta
     6661 ttaaaaaaac tggccacttt taaaatccag acttgtgaaa aatacattca agtactggaa
     6721 gaggtatgtg cgccgtttct gttatcacag tgtgcaatcc cattaccgca tatcagttat
     6781 aacaatagta atggtagcgc cattaaaaat attgtcggtt ctgcaactat cgcccaatac
     6841 cctactcttc cggaggaaaa tgtcaacaat atcagtgtta aatatgtttc tcctggctca
     6901 gtagggcctt cacctgtgcc attgaaatca ggagcaagtt tcagtgatct agtcaagctg
     6961 ttatctaacc gtccaccctc tcgtaactct ccagtgacaa taccaagaag cacaccttcg
     7021 catcgctcag tcacgccttt tctagggcaa cagcaacagc tgcaatcatt agtgccactg
     7081 accccgtctg ctttgtttgg tggcgccaat tttaatcaaa gtgggaatat tgctgatagc
     7141 tcattgtcct tcactttcac taacagtagc aacggtccga acctcataac aactcaaaca
     7201 aattctcaag cgctttcaca accaattgcc tcctctaacg ttcatgataa cttcatgaat
     7261 aatgaaatca cggctagtaa aattgatgat ggtaataatt caaaaccact gtcacctggt
     7321 tggacggacc aaactgcgta taacgcgttt ggaatcacta cagggatgtt taataccact
     7381 acaatggatg atgtatataa ctatctattc gatgatgaag ataccccacc aaacccaaaa
     7441 aaagagtaag cggccgcgtt aaccgatccg tcgaccatga agatcaagat cattgccccg
     7501 ccagagcgca agtactctgt ctggatcggt ggctccatcc tggcttcgct gtccaccttc
     7561 cagcagatgt ggatctccaa gcaggagtac gacgagtccg gcccctccat tgtgcaccgc
     7621 aagtgcttct aagaaggatc gcttgtctgg gcaagaggat caggatcggg atggtcttga
     7681 ttctgctgga ggaggaggag gagaagtcga ggaagcagca gcgaaagtgc aagtgcgagt
     7741 ggtggaagtt tggagtgcag cacaacaaaa tcaacaacaa caccaactac aagatgaaaa
     7801 gagcggaacc acctgcacac catcatcact atcatcatcg ttttgggcgc atgttgtgtg
     7861 gttccagcgt attaatataa ttaatttatt ccacatgaga tatgatatga tatactatgt
     7921 attttttgtt ttttttttat ttgtaaacct ttaatataac aagaactaca aaaaatgaaa
     7981 atgagcgaaa atgcatattc tgccattcca cacacacacc aacaacaccc aacacacgca
     8041 cacccacaag cttacacaca cacattcgcg gcatgacaag gacatcaaga taaagaagaa
     8101 cttaaagaag atatttccca aagcgcaaaa agaacacaca cacattgcaa aacacaaaca
     8161 acacactagc gttttgtaca attcgtcagc aaccttatgt attatttttt aattatgatg
     8221 taattataaa caaagtgaaa caaaaatatg aaaacaaaaa ggaaaatcaa atctgtcttc
     8281 tctttctccc gctctcctcg ctctctgctg ctaacctcgc cctctcctct ctcatctttt
     8341 tgtctgtctc tcttccacat ttttgccggc cggcaaaata ataacccaca cacactcaca
     8401 cttggctgca gtttcgcgtg cgatattcac acacattcaa gcatacaagc atacatacat
     8461 atgtattttt tttttatttg tacacttttc taattgcatg cgtatcgatt gataagttta
     8521 cgcctgaaaa tgttaattaa aatgtgaaaa tgcaactgaa aaactgatga aatgaaacaa
     8581 caacaagcga acaatttgct acatgtgtat tgtctaacaa ccgttactgc aacggttgct
     8641 tcggaaaagg gtgaagagga agagggcacg aggtcgacct gcaggcatgc aagcttggca
     8701 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     8761 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     8821 ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt
     8881 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
     8941 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     9001 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     9061 cagaggtttt caccgtcatc accgaaacgc gcga
//
