LOCUS       sequence-113779-         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lysosome-targeted FRET biosensor for monitoring mTORC1 kinase 
            activity; targeted to cytosolic face of lysosomal membrane..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Zhou X, Clister TL, Lowry PR, Seldin MM, Wong GW, Zhang J
  TITLE     Dynamic Visualization of mTORC1 Activity in Living Cells.
  JOURNAL   Cell Rep. 2015 Mar 11. pii: S2211-1247(15)00177-1. doi: 
            10.1016/j.celrep.2015.02.031.
  PUBMED    25772363
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2015 Mar 11. pii: S2211-1247(15)00177-1. doi: 
            10.1016/j.celrep.2015.02.031."
COMMENT     Sequence Label: pcDNA3-Lyso-TORCAR
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atcactctcg gcatggacga gctgtacaag cgcatgcata tgtccggggg cagcagctgc
       61 agccagaccc caagccgggc catccccgcc actcgccggg tggtgctcgg cgacggcgtg
      121 cagctcccgc ccggggacta cagcacgacc cccggcggca cgctcttcag caccaccccg
      181 ggaggtacca ggatcatcta tgaccggaaa ttcctgatgg agtgtcggaa ctcacctgtg
      241 accaaaacac ccccaaggga tctgcccacc attccggggg tcaccagccc ttccagtgat
      301 gagcccccca tggaagccag ccagagccac ctgcgcaata gcccagaaga taagcgggcg
      361 ggcggtgaag agtcacagtt tgagatggac attgagctct ctaaaggtga agaattattc
      421 actggtgttg tcccaatttt ggttgaatta gatggtgatg ttaatggtca caaattttct
      481 gtctccggtg aaggtgaagg tgatgctacg tacggtaaat tgaccttaaa attactctgt
      541 actactggta aattgccagt tccatggcca accttagtca ctactttagg ttatggtgtt
      601 caatgttttg ctagataccc agatcatatg aaacaacatg actttttcaa gtctgccatg
      661 ccagaaggtt atgttcaaga aagaactatt tttttcaaag atgacggtaa ctacaagacc
      721 agagctgaag tcaagtttga a
//
