LOCUS       sequence-11381-e        8950 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8950)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L
  TITLE     The Q system: a repressible binary system for transgene expression, 
            lineage tracing, and mosaic analysis.
  JOURNAL   Cell. 2010 Apr 30. 141(3):536-48.
  PUBMED    20434990
REFERENCE   2  (bases 1 to 8950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 30. 141(3):536-48."
COMMENT     Sequence Label: pQUAST
FEATURES             Location/Qualifiers
     source          1..8950
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(315..334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(994..1785,1786..1854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1617..1636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1855..1959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2027..2045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2083..2105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2205..2224
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(2761..2791,2792..2993)
                     /label=P element 3' end
                     /note="P element 3' end"
     promoter        3189..3427
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     3374..3395
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          3588..3653
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3783..3803
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4075..4209
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4112..4131)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4166..4185
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     gene            4227..8354
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(4236..4254)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(4705..4776,5171..5444,5519..6173,6235..6550,6771..6902,
                     6973..7587)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(8365..8919,8920..8950)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tgcggtgtga aataccgcac cgaatcgcgc ggaactaacg
     2281 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     2341 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     2401 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     2461 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     2521 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     2581 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     2641 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     2701 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     2761 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
     2821 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
     2881 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
     2941 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
     3001 gctgcatcca acgcgttggg agctctccgg atccaagctt ggatccgggt aatcgcttat
     3061 cctcggataa acaattatcc tcacgggtaa tcgcttatcc gctcgggtaa tcgcttatcc
     3121 tcgggtaatc gcttatcctt aagcttgata tcgaattgct gcagcccgag cggagactct
     3181 agcgagcgcc ggagtataaa tagaggcgct tcgtctacgg agcgacaatt caattcaaac
     3241 aagcaaagtg aacacgtcgc taagcgaaag ctaagcaaat aaacaagcgc agctgaacaa
     3301 gctaaacaat ctgcagtaaa gtgcaagtta aagtgaatca attaaaagta accagcaacc
     3361 aagtaaatca actgcaacta ctgaaatctg ccaagaagta attattgaat acaagaagag
     3421 aactctgaat agggaattgg gaattcgtta acagatcggg ggatcaattc gttaacagat
     3481 ctgcggccgc ggctcgaggg tacctctaga ggatctttgt gaaggaacct tacttctgtg
     3541 gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat
     3601 ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa
     3661 cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt
     3721 gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc
     3781 ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt
     3841 ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa
     3901 aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atatttgatg tatagtgcct
     3961 tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     4021 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     4081 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     4141 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     4201 gtctggatcc actagaaggc ctaattctag tatgtatgta agttaataaa acccattttt
     4261 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta
     4321 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat
     4381 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc
     4441 cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt
     4501 agcgttacgt agcgtcaatg tccgccttca gttgcttttg tcagcggttt cgtgacgaag
     4561 ctccaagcgg tttacgccat caattaaaca caaagtgctg tgccaaaact cctctcgctt
     4621 cttatttttg tttgtttttt gagtgattgg ggtggtgatt ggttttgggt gggtaagcag
     4681 gggaaagtgt gaaaaatccc ggcaatgggc caagaggatc aggagctatt aattcgcgga
     4741 ggcagcaaac acccatctgc cgagcatctg aacaatgtga gtagtacatg tgcatacatc
     4801 ttaagttcac ttgatctata ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga
     4861 actgcgaccc acaaaaatcc caaaccgcaa tcgcacaaac aaatagtgac acgaaacaga
     4921 ttattctggt agctgtgctc gctatataag acaattttta agatcatatc atgatcaaga
     4981 catctaaagg cattcatttt cgactacatt cttttttaca aaaaatataa caaccagata
     5041 ttttaagctg atcctagatg cacaaaaaat aaataaaagt ataaacctac ttcgtaggat
     5101 acttcgtttt gttcggggtt agatgagcat aacgcttgta gttgatattt gagatcccct
     5161 atcattgcag ggtgacagcg gagcggcttc gcagagctgc attaaccagg gcttcgggca
     5221 ggccaaaaac tacggcacgc tcctgccacc cagtccgccg gaggactccg gttcagggag
     5281 cggccaacta gccgagaacc tcacctatgc ctggcacaat atggacatct ttggggcggt
     5341 caatcagccg ggctccggat ggcggcagct ggtcaaccgg acacgcggac tattctgcaa
     5401 cgagcgacac ataccggcgc ccaggaaaca tttgctcaag aacggtgagt ttctattcgc
     5461 agtcggctga tctgtgtgaa atcttaataa agggtccaat taccaatttg aaactcagtt
     5521 tgcggcgtgg cctatccggg cgaacttttg gccgtgatgg gcagttccgg tgccggaaag
     5581 acgaccctgc tgaatgccct tgcctttcga tcgccgcagg gcatccaagt atcgccatcc
     5641 gggatgcgac tgctcaatgg ccaacctgtg gacgccaagg agatgcaggc caggtgcgcc
     5701 tatgtccagc aggatgacct ctttatcggc tccctaacgg ccagggaaca cctgattttc
     5761 caggccatgg tgcggatgcc acgacatctg acctatcggc agcgagtggc ccgcgtggat
     5821 caggtgatcc aggagctttc gctcagcaaa tgtcagcaca cgatcatcgg tgtgcccggc
     5881 agggtgaaag gtctgtccgg cggagaaagg aagcgtctgg cattcgcctc cgaggcacta
     5941 accgatccgc cgcttctgat ctgcgatgag cccacctccg gactggactc atttaccgcc
     6001 cacagcgtcg tccaggtgct gaagaagctg tcgcagaagg gcaagaccgt catcctgacc
     6061 attcatcagc cgtcttccga gctgtttgag ctctttgaca agatccttct gatggccgag
     6121 ggcagggtag ctttcttggg cactcccagc gaagccgtcg acttcttttc ctagtgagtt
     6181 cgatgtgttt attaagggta tctagcatta cattacatct caactcctat ccagcgtggg
     6241 tgcccagtgt cctaccaact acaatccggc ggacttttac gtacaggtgt tggccgttgt
     6301 gcccggacgg gagatcgagt cccgtgatcg gatcgccaag atatgcgaca attttgctat
     6361 tagcaaagta gcccgggata tggagcagtt gttggccacc aaaaatttgg agaagccact
     6421 ggagcagccg gagaatgggt acacctacaa ggccacctgg ttcatgcagt tccgggcggt
     6481 cctgtggcga tcctggctgt cggtgctcaa ggaaccactc ctcgtaaaag tgcgacttat
     6541 tcagacaacg gtgagtggtt ccagtggaaa caaatgatat aacgcttaca attcttggaa
     6601 acaaattcgc tagattttag ttagaattgc ctgattccac acccttctta gtttttttca
     6661 atgagatgta tagtttatag ttttgcagaa aataaataaa tttcatttaa ctcgcgaaca
     6721 tgttgaagat atgaatatta atgagatgcg agtaacattt taatttgcag atggttgcca
     6781 tcttgattgg cctcatcttt ttgggccaac aactcacgca agtgggcgtg atgaatatca
     6841 acggagccat cttcctcttc ctgaccaaca tgacctttca aaacgtcttt gccacgataa
     6901 atgtaagtct tgtttagaat acatttgcat attaataatt tactaacttt ctaatgaatc
     6961 gattcgattt aggtgttcac ctcagagctg ccagttttta tgagggaggc ccgaagtcga
     7021 ctttatcgct gtgacacata ctttctgggc aaaacgattg ccgaattacc gctttttctc
     7081 acagtgccac tggtcttcac ggcgattgcc tatccgatga tcggactgcg ggccggagtg
     7141 ctgcacttct tcaactgcct ggcgctggtc actctggtgg ccaatgtgtc aacgtccttc
     7201 ggatatctaa tatcctgcgc cagctcctcg acctcgatgg cgctgtctgt gggtccgccg
     7261 gttatcatac cattcctgct ctttggcggc ttcttcttga actcgggctc ggtgccagta
     7321 tacctcaaat ggttgtcgta cctctcatgg ttccgttacg ccaacgaggg tctgctgatt
     7381 aaccaatggg cggacgtgga gccgggcgaa attagctgca catcgtcgaa caccacgtgc
     7441 cccagttcgg gcaaggtcat cctggagacg cttaacttct ccgccgccga tctgccgctg
     7501 gactacgtgg gtctggccat tctcatcgtg agcttccggg tgctcgcata tctggctcta
     7561 agacttcggg cccgacgcaa ggagtagccg acatatatcc gaaataactg cttgtttttt
     7621 tttttaccat tattaccatc gtgtttactg tttattgccc cctcaaaaag ctaatgtaat
     7681 tatatttgtg ccaataaaaa caagatatga cctatagaat acaagtattt ccccttcgaa
     7741 catccccaca agtagacttt ggatttgtct tctaaccaaa agacttacac acctgcatac
     7801 cttacatcaa aaactcgttt atcgctacat aaaacaccgg gatatatttt ttatatacat
     7861 acttttcaaa tcgcgcgccc tcttcataat tcacctccac cacaccacgt ttcgtagttg
     7921 ctctttcgct gtctcccacc cgctctccgc aacacattca ccttttgttc gacgaccttg
     7981 gagcgactgt cgttagttcc gcgcgattcg gttcgctcaa atggttccga gtggttcatt
     8041 tcgtctcaat agaaattagt aataaatatt tgtatgtaca atttatttgc tccaatatat
     8101 ttgtatatat ttccctcaca gctatattta ttctaattta atattatgac tttttaaggt
     8161 aattttttgt gacctgttcg gagtgattag cgttacaatt tgaactgaaa gtgacatcca
     8221 gtgtttgttc cttgtgtaga tgcatctcaa aaaaatggtg ggcataatag tgttgtttat
     8281 atatatcaaa aataacaact ataataataa gaatacattt aatttagaaa atgcttggat
     8341 ttcactggaa ctagaattaa ttcggctgct gctctaaacg acgcatttcg tactccaaag
     8401 tacgaatttt ttccctcaag ctcttatttt cattaaacaa tgaacaggac ctaacgcaca
     8461 gtcacgttat tgtttacata aatgattttt tttactattc aaacttactc tgtttgtgta
     8521 ctcccactgg tatagccttc ttttatcttt tctggttcag gctctatcac tttactaggt
     8581 acggcatctg cgttgagtcg cctcctttta aatgtctgac cttttgcagg tgcagccttc
     8641 cactgcgaat cattaaagtg ggtatcacaa atttgggagt tttcaccaag gctgcaccca
     8701 aggctctgct cccacaattt tctcttaata gcacacttcg gcacgtgaat taattttact
     8761 ccagtcacag ctttgcagca aaatttgcaa tatttcattt ttttttattc cacgtaaggg
     8821 ttaatgtttt caaaaaaaaa ttcgtccgca cacaaccttt cctctcaaca agcaaacgtg
     8881 cactgaattt aagtgtatac ttcggtaagc ttcggctatc gacgggacca ccttatgtta
     8941 tttcatcatg
//
