LOCUS       sequence-11383-e       11134 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11134)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L
  TITLE     The Q system: a repressible binary system for transgene expression, 
            lineage tracing, and mosaic analysis.
  JOURNAL   Cell. 2010 Apr 30. 141(3):536-48.
  PUBMED    20434990
REFERENCE   2  (bases 1 to 11134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 30. 141(3):536-48."
COMMENT     Sequence Label: pQUAST-mCD8-GFP
FEATURES             Location/Qualifiers
     source          1..11134
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(315..334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(994..1785,1786..1854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1617..1636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1855..1959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2027..2045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2083..2105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2205..2224
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(2761..2791,2792..2993)
                     /label=P element 3' end
                     /note="P element 3' end"
     promoter        3189..3427
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     3374..3395
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             3503..4168
                     /codon_start=1
                     /product="mouse lymphocyte antigen CD8 alpha chain"
                     /label=mCD8
                     /translation="MASPLTRFLSLNLLLLGESIILGSGEAKPQAPELRIFPKKMDAEL
                     GQKVDLVCEVLGSVSQGCSWLFQNSSSKLPQPTFVVYMASSHNKITWDEKLNSSKLFSA
                     MRDTNNKYVLTLNKFSKENEGYYFCSVISNSVMYFSSVVPVLQKVNSTTTKPVLRTPSP
                     VHPTGTSQPQRPEDCRPRGSVKGTGLDFACDIYIWAPLAGICVALLLSLIITLICYHSR
                     "
     CDS             4175..4888
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(4205..4233)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4828..4848
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     intron          4975..5040
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5170..5190
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5462..5596
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     intron          5763..5828
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5958..5978
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6250..6384
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6411..6429)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(6889..6960,7355..7628,7703..8357,8419..8734,8955..9086,
                     9157..9771)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(10549..11103,11104..11134)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tgcggtgtga aataccgcac cgaatcgcgc ggaactaacg
     2281 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     2341 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     2401 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     2461 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     2521 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     2581 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     2641 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     2701 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     2761 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
     2821 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
     2881 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
     2941 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
     3001 ggctgcatca acgcgttggg agctctccgg atccaagctt ggatccgggt aatcgcttat
     3061 cctcggataa acaattatcc tcacgggtaa tcgcttatcc gctcgggtaa tcgcttatcc
     3121 tcgggtaatc gcttatcctt aagcttgata tcgaattcct gcagcccgag cggagactct
     3181 agcgagcgcc ggagtataaa tagaggcgct tcgtctacgg agcgacaatt caattcaaac
     3241 aagcaaagtg aacacgtcgc taagcgaaag ctaagcaaat aaacaagcgc agctgaacaa
     3301 gctaaacaat ctgcagtaaa gtgcaagtta aagtgaatca attaaaagta accagcaacc
     3361 aagtaaatca actgcaacta ctgaaatctg ccaagaagta attattgaat acaagaagag
     3421 aactctgaat agggaattgg gaattcgtta acagatcggg ggatcaattc gttaacagat
     3481 ctgcggccgc ggctcgagca aaatggcctc accgttgacc cgctttctgt cgctgaacct
     3541 gctgctgctg ggtgagtcga ttatcctggg gagtggagaa gctaagccac aggcacccga
     3601 actccgaatc tttccaaaga aaatggacgc cgaacttggt cagaaggtgg acctggtatg
     3661 tgaagtgttg gggtccgttt cgcaaggatg ctcttggctc ttccagaact ccagctccaa
     3721 actcccccag cccaccttcg ttgtctatat ggcttcatcc cacaacaaga taacgtggga
     3781 cgagaagctg aattcgtcga aactgttttc tgccatgagg gacacgaata ataagtacgt
     3841 tctcaccctg aacaagttca gcaaggaaaa cgaaggctac tatttctgct cagtcatcag
     3901 caactcggtg atgtacttca gttctgtcgt gccagtcctt cagaaagtga actctactac
     3961 taccaagcca gtgctgcgaa ctccctcacc tgtgcaccct accgggacat ctcagcccca
     4021 gagaccagaa gattgtcggc cccgtggctc agtgaagggg accggattgg acttcgcctg
     4081 tgatatttac atctgggcac ccttggccgg aatctgcgtg gcccttctgc tgtccttgat
     4141 catcactctc atctgctacc acagccgcgg atccatgagt aaaggagaag aacttttcac
     4201 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt
     4261 cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac
     4321 tactggaaaa ctacctgttc catggccaac acttgtcact actttaactt atggtgttca
     4381 atgcttttca agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc
     4441 cgaaggttat gtccaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg
     4501 tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga
     4561 ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa
     4621 tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca
     4681 caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg
     4741 cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa
     4801 agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat
     4861 tacacatggc atggatgaac tatacaaata atctagagga tctttgtgaa ggaaccttac
     4921 ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat
     4981 ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag
     5041 attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac
     5101 ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat
     5161 tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg
     5221 ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac
     5281 accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata tttgatgtat
     5341 agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt
     5401 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     5461 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     5521 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     5581 ttatcatgtc tggatccact agaaggcctt agtatggggt accccaaggg cgaattctgc
     5641 agatatccat cacactggcg gccgcggctc gagggtacct ctagaggatc tttgtgaagg
     5701 aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta
     5761 aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg
     5821 tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg
     5881 aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact
     5941 ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt
     6001 cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg
     6061 ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt
     6121 tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt agaggtttta
     6181 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     6241 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     6301 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     6361 aatgtatctt atcatgtctg gatcggatcc actagaaggc cttagtatgt atgtaagtta
     6421 ataaaaccct tttttggaga atgtagattt aaaaaaacat attttttttt tattttttac
     6481 tgcactggac atcattgaac ttatctgatc agttttaaat ttacttcgat ccaagggtat
     6541 ttgaagtacc aggttctttc gattacctct cactcaaaat gacattccac tcaaagtcag
     6601 cgctgtttgc ctccttctct gtccacagaa atatcgccgt ctctttcgcc gctgcgtccg
     6661 ctatctcttt cgccaccgtt tgtagcgtta cctagcgtca atgtccgcct tcagttgcac
     6721 tttgtcagcg gtttcgtgac gaagctccaa gcggtttacg ccatcaatta aacacaaagt
     6781 gctgtgccaa aactcctctc gcttcttatt tttgtttgtt ttttgagtga ttggggtggt
     6841 gattggtttt gggtgggtaa gcaggggaaa gtgtgaaaaa tcccggcaat gggccaagag
     6901 gatcaggagc tattaattcg cggaggcagc aaacacccat ctgccgagca tctgaacaat
     6961 gtgagtagta catgtgcata catcttaagt tcacttgatc tataggaact gcgattgcaa
     7021 catcaaattg tctgcggcgt gagaactgcg acccacaaaa atcccaaacc gcaatcgcac
     7081 aaacaaatag tgacacgaaa cagattattc tggtagctgt gctcgctata taagacaatt
     7141 tttaagatca tatcatgatc aagacatcta aaggcattca ttttcgacta cattcttttt
     7201 tacaaaaaat ataacaacca gatattttaa gctgatccta gatgcacaaa aaataaataa
     7261 aagtataaac ctacttcgta ggatacttcg ttttgttcgg ggttagatga gcataacgct
     7321 tgtagttgat atttgagatc ccctatcatt gcagggtgac agcggagcgg cttcgcagag
     7381 ctgcattaac cagggcttcg ggcaggccaa aaactacggc acgctcctgc cacccagtcc
     7441 gccggaggac tccggttcag ggagcggcca actagccgag aacctcacct atgcctggca
     7501 caatatggac atctttgggg cggtcaatca gccgggctcc ggatggcggc agctggtcaa
     7561 ccggacacgc ggactattct gcaacgagcg acacataccg gcgcccagga aacatttgct
     7621 caagaacggt gagtttctat tcgcagtcgg ctgatctgtg tgaaatctta ataaagggtc
     7681 caattaccaa tttgaaactc agtttgcggc gtggcctatc cgggcgaact tttggccgtg
     7741 atgggcagtt ccggtgccgg aaagacgacc ctgctgaatg cccttgcctt tcgatcgccg
     7801 cagggcatcc aagtatcgcc atccgggatg cgactgctca atggccaacc tgtggacgcc
     7861 aaggagatgc aggccaggtg cgcctatgtc cagcaggatg acctctttat cggctcccta
     7921 acggccaggg aacacctgat tttccaggcc atggtgcgga tgccacgaca tctgacctat
     7981 cggcagcgag tggcccgcgt ggatcaggtg atccaggagc tttcgctcag caaatgtcag
     8041 cacacgatca tcggtgtgcc cggcagggtg aaaggtctgt ccggcggaga aaggaagcgt
     8101 ctggcattcg cctccgaggc actaaccgat ccgccgcttc tgatctgcga tgagcccacc
     8161 tccggactgg actcatttac cgcccacagc gtcgtccagg tgctgaagaa gctgtcgcag
     8221 aagggcaaga ccgtcatcct gaccattcat cagccgtctt ccgagctgtt tgagctcttt
     8281 gacaagatcc ttctgatggc cgagggcagg gtagctttct tgggcactcc cagcgaagcc
     8341 gtcgacttct tttcctagtg agttcgatgt gtttattaag ggtatctagc attacattac
     8401 atctcaactc ctatccagcg tgggtgccca gtgtcctacc aactacaatc cggcggactt
     8461 ttacgtacag gtgttggccg ttgtgcccgg acgggagatc gagtcccgtg atcggatcgc
     8521 caagatatgc gacaattttg ctattagcaa agtagcccgg gatatggagc agttgttggc
     8581 caccaaaaat ttggagaagc cactggagca gccggagaat gggtacacct acaaggccac
     8641 ctggttcatg cagttccggg cggtcctgtg gcgatcctgg ctgtcggtgc tcaaggaacc
     8701 actcctcgta aaagtgcgac ttattcagac aacggtgagt ggttccagtg gaaacaaatg
     8761 atataacgct tacaattctt ggaaacaaat tcgctagatt ttagttagaa ttgcctgatt
     8821 ccacaccctt cttagttttt ttcaatgaga tgtatagttt atagttttgc agaaaataaa
     8881 taaatttcat ttaactcgcg aacatgttga agatatgaat attaatgaga tgcgagtaac
     8941 attttaattt gcagatggtt gccatcttga ttggcctcat ctttttgggc caacaactca
     9001 cgcaagtggg cgtgatgaat atcaacggag ccatcttcct cttcctgacc aacatgacct
     9061 ttcaaaacgt ctttgccacg ataaatgtaa gtcttgttta gaatacattt gcatattaat
     9121 aatttactaa ctttctaatg aatcgattcg atttaggtgt tcacctcaga gctgccagtt
     9181 tttatgaggg aggcccgaag tcgactttat cgctgtgaca catactttct gggcaaaacg
     9241 attgccgaat taccgctttt tctcacagtg ccactggtct tcacggcgat tgcctatccg
     9301 atgatcggac tgcgggccgg agtgctgcac ttcttcaact gcctggcgct ggtcactctg
     9361 gtggccaatg tgtcaacgtc cttcggatat ctaatatcct gcgccagctc ctcgacctcg
     9421 atggcgctgt ctgtgggtcc gccggttatc ataccattcc tgctctttgg cggcttcttc
     9481 ttgaactcgg gctcggtgcc agtatacctc aaatggttgt cgtacctctc atggttccgt
     9541 tacgccaacg agggtctgct gattaaccaa tgggcggacg tggagccggg cgaaattagc
     9601 tgcacatcgt cgaacaccac gtgccccagt tcgggcaagg tcatcctgga gacgcttaac
     9661 ttctccgccg ccgatctgcc gctggactac gtgggtctgg ccattctcat cgtgagcttc
     9721 cgggtgctcg catatctggc tctaagactt cgggcccgac gcaaggagta gccgacatat
     9781 atccgaaata actgcttgtt ttttttttta ccattattac catcgtgttt actgtttatt
     9841 gccccctcaa aaagctaatg taattatatt tgtgccaata aaaacaagat atgacctata
     9901 gaatacaagt atttcccctt cgaacatccc cacaagtaga ctttggattt gtcttctaac
     9961 caaaagactt acacacctgc ataccttaca tcaaaaactc gtttatcgct acataaaaca
    10021 ccgggatata ttttttatat acatactttt caaatcgcgc gccctcttca taattcacct
    10081 ccaccacacc acgtttcgta gttgctcttt cgctgtctcc cacccgctct ccgcaacaca
    10141 ttcacctttt gttcgacgac cttggagcga ctgtcgttag ttccgcgcga ttcggttcgc
    10201 tcaaatggtt ccgagtggtt catttcgtct caatagaaat tagtaataaa tatttgtatg
    10261 tacaatttat ttgctccaat atatttgtat atatttccct cacagctata tttattctaa
    10321 tttaatatta tgacttttta aggtaatttt ttgtgacctg ttcggagtga ttagcgttac
    10381 aatttgaact gaaagtgaca tccagtgttt gttccttgtg tagatgcatc tcaaaaaaat
    10441 ggtgggcata atagtgttgt ttatatatat caaaaataac aactataata ataagaatac
    10501 atttaattta gaaaatgctt ggatttcact ggaactagaa ttaattcggc tgctgctcta
    10561 aacgacgcat ttcgtactcc aaagtacgaa ttttttccct caagctctta ttttcattaa
    10621 acaatgaaca ggacctaacg cacagtcacg ttattgttta cataaatgat tttttttact
    10681 attcaaactt actctgtttg tgtactccca ctggtatagc cttcttttat cttttctggt
    10741 tcaggctcta tcactttact aggtacggca tctgcgttga gtcgcctcct tttaaatgtc
    10801 tgaccttttg caggtgcagc cttccactgc gaatcattaa agtgggtatc acaaatttgg
    10861 gagttttcac caaggctgca cccaaggctc tgctcccaca attttctctt aatagcacac
    10921 ttcggcacgt gaattaattt tactccagtc acagctttgc agcaaaattt gcaatatttc
    10981 attttttttt attccacgta agggttaatg ttttcaaaaa aaaattcgtc cgcacacaac
    11041 ctttcctctc aacaagcaaa cgtgcactga atttaagtgt atacttcggt aagcttcggc
    11101 tatcgacggg accaccttat gttatttcat catg
//
