LOCUS       sequence-113843-        1053 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombination-Induced Tag Exchange (RITE) Cassette, HA-6xHIS to a V5
            epitope tag, Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Terweij M, van Welsem T, van Deventer S, Verzijlbergen KF, 
            Menendez-Benito V, Ontoso D, San-Segundo P, Neefjes J, van Leeuwen F
  TITLE     Recombination-induced tag exchange (RITE) cassette series to monitor
            protein dynamics in Saccharomyces cerevisiae.
  JOURNAL   G3 (Bethesda). 2013 Aug 7;3(8):1261-72. doi: 10.1534/g3.113.006213.
  PUBMED    23708297
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.113"; 
            journalName: "G3 (Bethesda)"; date: "2013-08-7- 7"; volume: "3"; 
            issue: "8"; pages: "1261-72"
COMMENT     Sequence Label: pMT001
FEATURES             Location/Qualifiers
     source          1..1053
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    56..89
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             98..124
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             125..142
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      163..350
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(218..242)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     terminator      complement(469..666)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 gggcgaattg ggcccaatgc attggcgccg cggccgcggt ggatctggtg gatctataac
       61 ttcgtataat gtatgctata cgaagttatc aggtacctac ccatacgatg ttcctgacta
      121 tgcgcaccat catcaccatc acactagtta acttctaaat aagcgaattt cttatgattt
      181 atgattttta ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac
      241 tcttaggttt taaaacgaaa attcttattc ttgagtaact ctttcctgta ggtcaggttg
      301 ctttctcagg tatagtatga ggtcgctctt attgaccaca cctctaccgg cagatccgct
      361 agggataaca gggtaaggat ccgcattgta ctgagagtgc accataatca tcgatgaatt
      421 cgagctcgtt ttcgacactg gatggcggcg ttagtatcga atcgacagca gtatagcgac
      481 cagcattcac atacgattga cgcatgatat tactttctgc gcacttaact tcgcatctgg
      541 gcagatgatg tcgaggcgaa aaaaaatata aatcacgcta acatttgatt aaaatagaac
      601 aactacaata taaaaaaact atacaaatga caagttcttg aaaacaagaa tctttttatt
      661 gtcagtactg attattcctt tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg
      721 gcgagtactt ctacacagcc atcggtccag acggccgcgc ttctgcgggc gatttgtgta
      781 cgcccgacag tcccggctcc ggatcggacg attgcgtcgc atcgaccctg cgcccaagct
      841 gcatcatcga aattgccgtc aaccaagctc tgatagagtt ggtcaagacc aatgcggagc
      901 atatacgccc ggagccgcgg cgatcctgca agctccggat gcctccgctc gaagtagcgc
      961 gtctgctgct ccatacaagc caaccacggc ctccagaaga agatgttggc gacctcgtat
     1021 tgggaatccc cgaacatcgc ctcgctccag tca
//
