LOCUS       sequence-113849-         477 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E coli Genetic Modification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Yang J, Sun B, Huang H, Jiang Y, Diao L, Chen B, Xu C, Wang X, Liu 
            J, Jiang W, Yang S
  TITLE     High-Efficiency Scarless Genetic Modification in Escherichia coli by
            Using Lambda Red Recombination and I-SceI Cleavage.
  JOURNAL   Appl Environ Microbiol. 2014 Jul 1;80(13):3826-3834. Epub 2014 Apr 
            18.
  PUBMED    24747889
REFERENCE   2  (bases 1 to 477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol."; date: "2014-07-1"; volume: "80(13)"; pages: 
            "3826-3834. Epub 2014 Apr 18"
COMMENT     Sequence Label: pREDTAI
FEATURES             Location/Qualifiers
     source          1..477
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tgcgggatca ggtacggttt gatcaggttg tagaagatca ggtaagacat agaatcgatg
       61 tagatgatcg gtttgttttt gttgattttt acgtaacagt tcagttggaa tttgttacgc
      121 agacccttaa ccaggtattc tacttcttcg aaagtgaaag actgggtgtt cagtacgatc
      181 gatttgttgg tagagttttt gttgtaatcc catttaccac catcatccat gaaccagtat
      241 gccagagaca tcggggtcag gtagttttca accaggttgt tcgggatggt ttttttgttg
      301 ttaacgatga acaggttagc cagtttgttg aaagcttggt gtttgaaagt ctgggcgccc
      361 caggtgatta ccaggttacc caggtggtta acacgttctt ttttgtgcgg cggggacagt
      421 acccactgat cgtacagcag acatacgtgg tccatgtatg ctttgttttt ccactcg
//
