LOCUS       sequence-113850-        1095 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombination-Induced Tag Exchange (RITE) Cassette, HA-6xHIS to a V5
            epitope tag, Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Terweij M, van Welsem T, van Deventer S, Verzijlbergen KF, 
            Menendez-Benito V, Ontoso D, San-Segundo P, Neefjes J, van Leeuwen F
  TITLE     Recombination-induced tag exchange (RITE) cassette series to monitor
            protein dynamics in Saccharomyces cerevisiae.
  JOURNAL   G3 (Bethesda). 2013 Aug 7;3(8):1261-72. doi: 10.1534/g3.113.006213.
  PUBMED    23708297
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.113"; 
            journalName: "G3 (Bethesda)"; date: "2013-08-7- 7"; volume: "3"; 
            issue: "8"; pages: "1261-72"
COMMENT     Sequence Label: pMT001
FEATURES             Location/Qualifiers
     source          1..1095
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        8..26
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    33..89
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     protein_bind    complement(154..187)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        282..625
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 agctcggaat taaccctcac taaagggaac aaaagcttgc atgcctgcag gtcgactcta
       61 gaggatcccc gggtaccgag ctcgaattcg cggccgctta tgtacaagaa tcgagaccga
      121 ggagagggtt agggataggc ttaccgtcga ctgataactt cgtatagcat acattatacg
      181 aagttattct agactgtgcg gtatttcaca ccgcataggg ttaattaagg cgcgccagat
      241 ctgtttagct tgcctcgtcc ccgccgggtc acccggccag cgacatggag gcccagaata
      301 ccctccttga cagtcttgac gtgcgcagct caggggcatg atgtgactgt cgcccgtaca
      361 tttagcccat acatccccat gtataatcat ttgcatccat acattttgat ggccgcacgg
      421 cgcgaagcaa aaattacggc tcctcgctgc ggacctgcga gcagggaaac gctcccctca
      481 cagacgcgtt gaattgtccc cacgccgcgc ccctgtagag aaatataaaa ggttaggatt
      541 tgccactgag gttcttcttt catatacttc cttttaaaat cttgctagga tacagttctc
      601 acatcacatc cgaacataaa caaccatggg taaaaagcct gaactcaccg cgacgtctgt
      661 cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg
      721 cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa
      781 tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc
      841 gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat
      901 ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt
      961 tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag
     1021 cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat
     1081 atgcgcgatt gctga
//
