LOCUS       sequence-113855-         612 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  visualizing calcium in cultured Drosophila S2 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Gao XJ, Riabinina O, Li J, Potter CJ, Clandinin TR, Luo L
  TITLE     A transcriptional reporter of intracellular Ca in Drosophila.
  JOURNAL   Nat Neurosci. 2015 May 11. doi: 10.1038/nn.4016.
  PUBMED    25961791
REFERENCE   2  (bases 1 to 612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2015 May 11. doi: 10.1038/nn.4016."
COMMENT     Sequence Label: pActP-GCaMP6m
FEATURES             Location/Qualifiers
     source          1..612
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(74..95)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(308..364)
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     regulatory      376..385
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(join(392..406,407..415))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(419..451)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(455..472)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ccttgctcac catgctccct ccggtaccgc ccttgtacag ctcgtccatg ccgagagtga
       61 tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt
      121 tcgaaagttt ggactgcacg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc
      181 cgatgggggt gttctgctgg tagtggtagg cgagctgcac gccgccgtcc tcgatgttgt
      241 ggcggatctt gaagttcgcc ttgatgccgt tcttctgctt gtcggccttg atatagacgt
      301 tctcgagtga gctcagccga cctatagctc tgactgcgtg acctgtctta ttccacttac
      361 gacgtgatga gtcgaccatg gtggcgagat ccttatcgtc atcgtcgtac agatcccgac
      421 ccatttgctg tccaccagtc atgctagcca taccatgatg atgatgatga tgagaaccca
      481 ttttgcggcc gcagatcgga tccggggtct ctggattaga cgactgctgg ctgatggagc
      541 ggctttgtgt cgggaggagt atccacacag cacaagaact caaacggtag tgatatgaaa
      601 actggtcccg ca
//
