LOCUS       sequence-113857-       10760 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAVS1 safe harbor gene targeting donor expressing CAG-driven copGFP,
            compatible with pZT-AAVS1 TALENs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10760)
  AUTHORS   Cerbini T, Funahashi R, Luo Y, Liu C, Park K, Rao M, Malik N, Zou J
  TITLE     Transcription activator-like effector nuclease (TALEN)-mediated 
            CLYBL targeting enables enhanced transgene expression and one-step 
            generation of dual reporter human induced pluripotent stem cell 
            (iPSC) and neural stem cell (NSC) lines.
  JOURNAL   PLoS One. 2015 Jan 14;10(1):e0116032. doi: 
            10.1371/journal.pone.0116032. eCollection 2015.
  PUBMED    25587899
REFERENCE   2  (bases 1 to 10760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-01-14"; volume: "10(1):e0116032. doi"; pages: " 
            10.1371/journal.pone.0116032. eCollection 2015"
COMMENT     Sequence Label: pAAVS1P-iCAG.copGFP
FEATURES             Location/Qualifiers
     source          1..10760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(103..122)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             114..923
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(181..200)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1101..1689
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1590..1609
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1843..1860
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1977..1998
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2013..2018,2019..2036,2037..2043)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2051..2067
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2056..2078
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2075..2091
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2075..2091
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    2112..2915
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     protein_bind    complement(2924..2957)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    2958..2983
                     /label=SA
                     /note="splice acceptor site"
     CDS             3007..3060
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3064..3663
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3064..3083)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3560..3580
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    3664..3888
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(3889..3922)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(5166..5199)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     enhancer        5270..5573
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5575..5852
                     /label=chicken beta-actin promoter
     intron          5854..6871
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     6795..6817
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     6879..6898
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             6959..7621
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     primer_bind     complement(7783..7802)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7848..7903
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7902..7921)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     protein_bind    8236..8269
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     misc_feature    9521..10357
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(10366..10383)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10366..10382)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10375..10397)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(10591..10610)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10710..10732
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 tgcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
       61 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc
      121 atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg
      181 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg
      241 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg
      301 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca
      361 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa
      421 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg
      481 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc
      541 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg
      601 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac
      661 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta
      721 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc
      781 gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga
      841 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt
      901 tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacat tattcagatt
      961 gggcttgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     1021 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1081 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     1141 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     1201 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     1261 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     1321 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     1381 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     1441 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     1501 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     1561 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     1621 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     1681 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     1741 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     1801 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     1861 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     1921 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     1981 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     2041 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     2101 gccgcggccg ctgctttctc tgaccagcat tctctcccct gggcctgtgc cgctttctgt
     2161 ctgcagcttg tggcctgggt cacctctacg gctggcccag atccttccct gccgcctcct
     2221 tcaggttccg tcttcctcca ctccctcttc cccttgctct ctgctgtgtt gctgcccaag
     2281 gatgctcttt ccggagcact tccttctcgg cgctgcacca cgtgatgtcc tctgagcgga
     2341 tcctccccgt gtctgggtcc tctccgggca tctctcctcc ctcacccaac cccatgccgt
     2401 cttcactcgc tgggttccct tttccttctc cttctggggc ctgtgccatc tctcgtttct
     2461 taggatggcc ttctccgacg gatgtctccc ttgcgtcccg cctccccttc ttgtaggcct
     2521 gcatcatcac cgtttttctg gacaacccca aagtaccccg tctccctggc tttagccacc
     2581 tctccatcct cttgctttct ttgcctggac accccgttct cctgtggatt cgggtcacct
     2641 ctcactcctt tcatttgggc agctccccta ccccccttac ctctctagtc tgtgctagct
     2701 cttccagccc cctgtcatgg catcttccag gggtccgaga gctcagctag tcttcttcct
     2761 ccaacccggg cccctatgtc cacttcagga cagcatgttt gctgcctcca gggatcctgt
     2821 gtccccgagc tgggaccacc ttatattccc agggccggtt aatgtggctc tggttctggg
     2881 tacttttatc tgtcccctcc accccacagt ggggcgcggc cgcataactt cgtatagcat
     2941 acattatacg aagttatctg acctcttctc ttcctcccac agggcctcga gagatctggc
     3001 agcggagagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct
     3061 aggatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     3121 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     3181 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     3241 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     3301 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     3361 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     3421 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     3481 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     3541 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     3601 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     3661 tgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     3721 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     3781 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     3841 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggat aacttcgtat
     3901 agcatacatt atacgaagtt atgagctcac ggggacagcc cccccccaaa gcccccaggg
     3961 atgtaattac gtccctcccc cgctaggggg cagcagcgag ccgcccgggg ctccgctccg
     4021 gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg gggacagccc gggcacgggg
     4081 aaggtggcac gggatcgctt tcctctgaac gcttctcgct gctctttgag cctgcagaca
     4141 cctgggggga tacggggaaa aagctttagg ctgaaagaga gatttagaat gacagaatca
     4201 tagaacggcc tgggttgcaa aggagcacag tgctcatcca gatccaaccc cctgctatgt
     4261 gcagggtcat caaccagcag cccaggctgc ccagagccac atccagcctg gccttgaatg
     4321 cctgcaggga tggggcatcc acagcctcct tgggcaacct gttcagtgcg tcaccaccct
     4381 ctgggggaaa aactgcctcc tcatatccaa cccaaacctc ccctgtctca gtgtaaagcc
     4441 attccccctt gtcctatcaa gggggagttt gctgtgacat tgttggtctg gggtgacaca
     4501 tgtttgccaa ttcagtgcat cacggagagg cagatcttgg ggataaggaa gtgcaggaca
     4561 gcatggacgt gggacatgca ggtgttgagg gctctgggac actctccaag tcacagcgtt
     4621 cagaacagcc ttaaggataa gaagatagga tagaaggaca aagagcaagt taaaacccag
     4681 catggagagg agcacaaaaa ggccacagac actgctggtc cctgtgtctg agcctgcatg
     4741 tttgatggtg tctggatgca agcagaaggg gtggaagagc ttgcctggag agatacagct
     4801 gggtcagtag gactgggaca ggcagctgga gaattgccat gtagatgttc atacaatcgt
     4861 caaatcatga aggctggaaa agccctccaa gatccccaag accaacccca acccacccac
     4921 cgtgcccact ggccatgtcc ctcagtgcca catccccaca gttcttcatc acctccaggg
     4981 acggtgaccc ccccacctcc gtgggcagct gtgccactgc agcaccgctc tttggagaag
     5041 gtaaatcttg ctaaatccag cccgaccctc ccctggcaca acgtaaggcc attatctctc
     5101 atccaactcc aggacggagt cagtgagaat attggcgcgc cgaattctcg cgacgatcgg
     5161 tcgacataac ttcgtatagg atactttata cgaagttatc aattcaggct agttattaat
     5221 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     5281 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     5341 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     5401 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     5461 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     5521 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg
     5581 tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt
     5641 atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcgcg
     5701 cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg
     5761 cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc
     5821 ggccctataa aaagcgaagc gcgcggcggg cggggagtcg ctgcgacgct gccttcgccc
     5881 cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact
     5941 cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta
     6001 atgacggctt gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc
     6061 tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc
     6121 gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc
     6181 gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggg
     6241 ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt
     6301 gggcgcgtcg gtcgggctgc aaccccccct gcacccccct ccccgagttg ctgagcacgg
     6361 cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg
     6421 ggggtggcgg caggtggggg tgccgggcgg ggcggggccg cctcgggccg gggagggctc
     6481 gggggagggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag
     6541 ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg
     6601 tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg
     6661 gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg
     6721 tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg
     6781 acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa
     6841 ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct
     6901 gtctcatcat tttggcaaag aattgtgtac aaccatggag agcgacgaga gcggcctgcc
     6961 cgccatggag atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt
     7021 gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa
     7081 aggcgccctg accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca
     7141 cttcggcacc taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg
     7201 ctacaccaac acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag
     7261 ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc
     7321 cgaggacagc gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct
     7381 gcaccccatg ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga
     7441 cggcggctac tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc
     7501 cagcatcctg cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag
     7561 caacaccgag ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc
     7621 cagatcccgc gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac
     7681 cggatctcgc taaacgcgtg aattcactcc tcaggtgcag gctgcctatc agaaggtggt
     7741 ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa
     7801 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt
     7861 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
     7921 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc
     7981 ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca
     8041 gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt
     8101 tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt
     8161 actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt
     8221 atggagatca ccggtataac ttcgtataat gtatactata cgaagttatg gtaccttaat
     8281 taagttaaca tgcatagtac tgagctcacg gggacagccc ccccccaaag cccccaggga
     8341 tgtaattacg tccctccccc gctagggggc agcagcgagc cgcccggggc tccgctccgg
     8401 tccggcgctc cccccgcatc cccgagccgg cagcgtgcgg ggacagcccg ggcacgggga
     8461 aggtggcacg ggatcgcttt cctctgaacg cttctcgctg ctctttgagc ctgcagacac
     8521 ctggggggat acggggaaaa agctttaggc tgaaagagag atttagaatg acagaatcat
     8581 agaacggcct gggttgcaaa ggagcacagt gctcatccag atccaacccc ctgctatgtg
     8641 cagggtcatc aaccagcagc ccaggctgcc cagagccaca tccagcctgg ccttgaatgc
     8701 ctgcagggat ggggcatcca cagcctcctt gggcaacctg ttcagtgcgt caccaccctc
     8761 tgggggaaaa actgcctcct catatccaac ccaaacctcc cctgtctcag tgtaaagcca
     8821 ttcccccttg tcctatcaag ggggagtttg ctgtgacatt gttggtctgg ggtgacacat
     8881 gtttgccaat tcagtgcatc acggagaggc agatcttggg gataaggaag tgcaggacag
     8941 catggacgtg ggacatgcag gtgttgaggg ctctgggaca ctctccaagt cacagcgttc
     9001 agaacagcct taaggataag aagataggat agaaggacaa agagcaagtt aaaacccagc
     9061 atggagagga gcacaaaaag gccacagaca ctgctggtcc ctgtgtctga gcctgcatgt
     9121 ttgatggtgt ctggatgcaa gcagaagggg tggaagagct tgcctggaga gatacagctg
     9181 ggtcagtagg actgggacag gcagctggag aattgccatg tagatgttca tacaatcgtc
     9241 aaatcatgaa ggctggaaaa gccctccaag atccccaaga ccaaccccaa cccacccacc
     9301 gtgcccactg gccatgtccc tcagtgccac atccccacag ttcttcatca cctccaggga
     9361 cggtgacccc cccacctccg tgggcagctg tgccactgca gcaccgctct ttggagaagg
     9421 taaatcttgc taaatccagc ccgaccctcc cctggcacaa cgtaaggcca ttatctctca
     9481 tccaactcca ggacggagtc agtgagaata ttgtttaaac actagggaca ggattggtga
     9541 cagaaaagcc ccatccttag gcctcctcct tcctagtctc ctgatattgg gtctaacccc
     9601 cacctcctgt taggcagatt ccttatctgg tgacacaccc ccatttcctg gagccatctc
     9661 tctccttgcc agaacctcta aggtttgctt acgatggagc cagagaggat cctgggaggg
     9721 agagcttggc agggggtggg agggaagggg gggatgcgtg acctgcccgg ttctcagtgg
     9781 ccaccctgcg ctaccctctc ccagaacctg agctgctctg acgcggctgt ctggtgcgtt
     9841 tcactgatcc tggtgctgca gcttccttac acttcccaag aggagaagca gtttggaaaa
     9901 acaaaatcag aataagttgg tcctgagttc taactttggc tcttcacctt tctagtcccc
     9961 aatttatatt gttcctccgt gcgtcagttt tacctgtgag ataaggccag tagccagccc
    10021 cgtcctggca gggctgtggt gaggaggggg gtgtccgtgt ggaaaactcc ctttgtgaga
    10081 atggtgcgtc ctaggtgttc accaggtcgt ggccgcctct actccctttc tctttctcca
    10141 tccttctttc cttaaagagt ccccagtgct atctgggaca tattcctccg cccagagcag
    10201 ggtcccgctt ccctaaggcc ctgctctggg cttctgggtt tgagtccttg gcaagcccag
    10261 gagaggcgct caggcttccc tgtccccctt cctcgtccac catctcatgc ccctggctct
    10321 cctgcccctt ccctacaggg gttcctggct ctgctctgtt taaacactgg ccgtcgtttt
    10381 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
    10441 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
    10501 gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
    10561 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
    10621 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
    10681 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
    10741 gtcatcaccg aaacgcgcga
//
