LOCUS       sequence-113858-        1040 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombination-Induced Tag Exchange (RITE) Cassette, 3xHA to a 3xT7 
            epitope tag, Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Terweij M, van Welsem T, van Deventer S, Verzijlbergen KF, 
            Menendez-Benito V, Ontoso D, San-Segundo P, Neefjes J, van Leeuwen F
  TITLE     Recombination-induced tag exchange (RITE) cassette series to monitor
            protein dynamics in Saccharomyces cerevisiae.
  JOURNAL   G3 (Bethesda). 2013 Aug 7;3(8):1261-72. doi: 10.1534/g3.113.006213.
  PUBMED    23708297
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.113"; 
            journalName: "G3 (Bethesda)"; date: "2013-08-7- 7"; volume: "3"; 
            issue: "8"; pages: "1261-72"
COMMENT     Sequence Label: pFvl160
FEATURES             Location/Qualifiers
     source          1..1040
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    56..89
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             98..124
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             131..157
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             161..187
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      208..395
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(263..287)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     terminator      complement(514..711)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 gggcgaattg ggcccaatgc attggcgccg cggccgcggt ggatctggtg gatctataac
       61 ttcgtataat gtatgctata cgaagttatc aggtacctac ccatacgatg ttcctgacta
      121 tgcgactagc tatccctatg acgtcccgga ctatgcagga tatccatatg acgttccaga
      181 ttacgctact agttaacttc taaataagcg aatttcttat gatttatgat ttttattatt
      241 aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta ggttttaaaa
      301 cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc tcaggtatag
      361 tatgaggtcg ctcttattga ccacacctct accggcagat ccgctaggga taacagggta
      421 aggatccaga ttgtactgag agtgcaccat aatcatcgat gaattcgagc tcgttttcga
      481 cactggatgg cggcgttagt atcgaatcga cagcagtata gcgaccagca ttcacatacg
      541 attgacgcat gatattactt tctgcgcact taacttcgca tctgggcaga tgatgtcgag
      601 gcgaaaaaaa atataaatca cgctaacatt tgattaaaat agaacaacta caatataaaa
      661 aaactataca aatgacaagt tcttgaaaac aagaatcttt ttattgtcag tactgattat
      721 tcctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca
      781 cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg
      841 gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg
      901 ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagc
      961 cgcggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata
     1021 caagccaacc acggcctcca
//
