LOCUS       sequence-11386-e       10527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10527)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L
  TITLE     The Q system: a repressible binary system for transgene expression, 
            lineage tracing, and mosaic analysis.
  JOURNAL   Cell. 2010 Apr 30. 141(3):536-48.
  PUBMED    20434990
REFERENCE   2  (bases 1 to 10527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 30. 141(3):536-48."
COMMENT     Sequence Label: pQUAST-mtdT-3XHA
FEATURES             Location/Qualifiers
     source          1..10527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(315..334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(994..1785,1786..1854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1617..1636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1855..1959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2027..2045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2083..2105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2205..2224
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(2761..2791,2792..2993)
                     /label=P element 3' end
                     /note="P element 3' end"
     promoter        3189..3427
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     3374..3395
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             3507..4934
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             4935..5024
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     primer_bind     complement(5048..5064)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5049..5065)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     intron          5156..5221
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5351..5371
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5643..5777
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5680..5699)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5734..5753
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5804..5822)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(6282..6353,6748..7021,7096..7750,7812..8127,8348..8479,
                     8550..9164)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(9942..10496,10497..10527)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tgcggtgtga aataccgcac cgaatcgcgc ggaactaacg
     2281 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     2341 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     2401 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     2461 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     2521 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     2581 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     2641 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     2701 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     2761 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
     2821 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
     2881 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
     2941 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
     3001 gctgcatcca acgcgttggg agctctccgg atccaagctt ggatccgggt aatcgcttat
     3061 cctcggataa acaattatcc tcacgggtaa tcgcttatcc gctcgggtaa tcgcttatcc
     3121 tcgggtaatc gcttatcctt aagcttgata tcgaattcct gcagcccgag cggagactct
     3181 agcgagcgcc ggagtataaa tagaggcgct tcgtctacgg agcgacaatt caattcaaac
     3241 aagcaaagtg aacacgtcgc taagcgaaag ctaagcaaat aaacaagcgc agctgaacaa
     3301 gctaaacaat ctgcagtaaa gtgcaagtta aagtgaatca attaaaagta accagcaacc
     3361 aagtaaatca actgcaacta ctgaaatctg ccaagaagta attattgaat acaagaagag
     3421 aactctgaat agggaattgg gaattcgtta acagatcggg ggatcaattc gttaacagat
     3481 ccatgggttg ctgtttctcc aagaccatgg tgagcaaggg agaggaggtc atcaaagagt
     3541 tcatgcgctt caaggtgcgc atggagggct ccatgaacgg ccacgagttc gagatcgagg
     3601 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
     3661 gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttcatgtac ggctccaagg
     3721 cgtacgtgaa gcaccccgcc gacatccccg attacaagaa gctgtccttc cccgagggct
     3781 tcaagtggga gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg acccaggact
     3841 cctccctgca ggacggcacg ctgatctaca aggtgaagat gcgcggcacc aacttccccc
     3901 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccacc gagcgcctgt
     3961 acccccgcga cggcgtgctg aagggcgaga tccaccaggc cctgaagctg aaggacggcg
     4021 gccactacct ggtggagttc aagaccatct acatggccaa gaagcccgtg caactgcccg
     4081 gctactacta cgtggacacc aagctggaca tcacctccca caacgaggac tacaccatcg
     4141 tggaacagta cgagcgctcc gagggccgcc accacctgtt cctggggcat ggcaccggca
     4201 gcaccggcag cggcagctcc ggcaccgcct cctccgagga caacaacatg gccgtcatca
     4261 aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga
     4321 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
     4381 ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct
     4441 ccaaggcgta cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg
     4501 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc
     4561 aggactcctc cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact
     4621 tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc
     4681 gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg
     4741 acggcggccg ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac
     4801 tgcccggcta ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca
     4861 ccatcgtgga acagtacgag cgctccgagg gccgccacca cctgttcctg tacggcatgg
     4921 acgagctgta caagtaccca tacgatgttc ctgactatgc gggctatccc tatgacgtcc
     4981 cggactatgc aggatcctat ccatatgacg ttccagatta cgcttaagaa ttcgatatca
     5041 agcttatcga taccgtcgac ctcgagggta cctctagagg atctttgtga aggaacctta
     5101 cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa
     5161 tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta
     5221 gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa
     5281 cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca
     5341 ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt
     5401 gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta
     5461 caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat atttgatgta
     5521 tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     5581 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     5641 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     5701 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     5761 cttatcatgt ctggatcgga tccactagaa ggccttagta tgtatgtaag ttaataaaac
     5821 ccttttttgg agaatgtaga tttaaaaaaa catatttttt ttttattttt tactgcactg
     5881 gacatcattg aacttatctg atcagtttta aatttacttc gatccaaggg tatttgaagt
     5941 accaggttct ttcgattacc tctcactcaa aatgacattc cactcaaagt cagcgctgtt
     6001 tgcctccttc tctgtccaca gaaatatcgc cgtctctttc gccgctgcgt ccgctatctc
     6061 tttcgccacc gtttgtagcg ttacctagcg tcaatgtccg ccttcagttg cactttgtca
     6121 gcggtttcgt gacgaagctc caagcggttt acgccatcaa ttaaacacaa agtgctgtgc
     6181 caaaactcct ctcgcttctt atttttgttt gttttttgag tgattggggt ggtgattggt
     6241 tttgggtggg taagcagggg aaagtgtgaa aaatcccggc aatgggccaa gaggatcagg
     6301 agctattaat tcgcggaggc agcaaacacc catctgccga gcatctgaac aatgtgagta
     6361 gtacatgtgc atacatctta agttcacttg atctatagga actgcgattg caacatcaaa
     6421 ttgtctgcgg cgtgagaact gcgacccaca aaaatcccaa accgcaatcg cacaaacaaa
     6481 tagtgacacg aaacagatta ttctggtagc tgtgctcgct atataagaca atttttaaga
     6541 tcatatcatg atcaagacat ctaaaggcat tcattttcga ctacattctt ttttacaaaa
     6601 aatataacaa ccagatattt taagctgatc ctagatgcac aaaaaataaa taaaagtata
     6661 aacctacttc gtaggatact tcgttttgtt cggggttaga tgagcataac gcttgtagtt
     6721 gatatttgag atcccctatc attgcagggt gacagcggag cggcttcgca gagctgcatt
     6781 aaccagggct tcgggcaggc caaaaactac ggcacgctcc tgccacccag tccgccggag
     6841 gactccggtt cagggagcgg ccaactagcc gagaacctca cctatgcctg gcacaatatg
     6901 gacatctttg gggcggtcaa tcagccgggc tccggatggc ggcagctggt caaccggaca
     6961 cgcggactat tctgcaacga gcgacacata ccggcgccca ggaaacattt gctcaagaac
     7021 ggtgagtttc tattcgcagt cggctgatct gtgtgaaatc ttaataaagg gtccaattac
     7081 caatttgaaa ctcagtttgc ggcgtggcct atccgggcga acttttggcc gtgatgggca
     7141 gttccggtgc cggaaagacg accctgctga atgcccttgc ctttcgatcg ccgcagggca
     7201 tccaagtatc gccatccggg atgcgactgc tcaatggcca acctgtggac gccaaggaga
     7261 tgcaggccag gtgcgcctat gtccagcagg atgacctctt tatcggctcc ctaacggcca
     7321 gggaacacct gattttccag gccatggtgc ggatgccacg acatctgacc tatcggcagc
     7381 gagtggcccg cgtggatcag gtgatccagg agctttcgct cagcaaatgt cagcacacga
     7441 tcatcggtgt gcccggcagg gtgaaaggtc tgtccggcgg agaaaggaag cgtctggcat
     7501 tcgcctccga ggcactaacc gatccgccgc ttctgatctg cgatgagccc acctccggac
     7561 tggactcatt taccgcccac agcgtcgtcc aggtgctgaa gaagctgtcg cagaagggca
     7621 agaccgtcat cctgaccatt catcagccgt cttccgagct gtttgagctc tttgacaaga
     7681 tccttctgat ggccgagggc agggtagctt tcttgggcac tcccagcgaa gccgtcgact
     7741 tcttttccta gtgagttcga tgtgtttatt aagggtatct agcattacat tacatctcaa
     7801 ctcctatcca gcgtgggtgc ccagtgtcct accaactaca atccggcgga cttttacgta
     7861 caggtgttgg ccgttgtgcc cggacgggag atcgagtccc gtgatcggat cgccaagata
     7921 tgcgacaatt ttgctattag caaagtagcc cgggatatgg agcagttgtt ggccaccaaa
     7981 aatttggaga agccactgga gcagccggag aatgggtaca cctacaaggc cacctggttc
     8041 atgcagttcc gggcggtcct gtggcgatcc tggctgtcgg tgctcaagga accactcctc
     8101 gtaaaagtgc gacttattca gacaacggtg agtggttcca gtggaaacaa atgatataac
     8161 gcttacaatt cttggaaaca aattcgctag attttagtta gaattgcctg attccacacc
     8221 cttcttagtt tttttcaatg agatgtatag tttatagttt tgcagaaaat aaataaattt
     8281 catttaactc gcgaacatgt tgaagatatg aatattaatg agatgcgagt aacattttaa
     8341 tttgcagatg gttgccatct tgattggcct catctttttg ggccaacaac tcacgcaagt
     8401 gggcgtgatg aatatcaacg gagccatctt cctcttcctg accaacatga cctttcaaaa
     8461 cgtctttgcc acgataaatg taagtcttgt ttagaataca tttgcatatt aataatttac
     8521 taactttcta atgaatcgat tcgatttagg tgttcacctc agagctgcca gtttttatga
     8581 gggaggcccg aagtcgactt tatcgctgtg acacatactt tctgggcaaa acgattgccg
     8641 aattaccgct ttttctcaca gtgccactgg tcttcacggc gattgcctat ccgatgatcg
     8701 gactgcgggc cggagtgctg cacttcttca actgcctggc gctggtcact ctggtggcca
     8761 atgtgtcaac gtccttcgga tatctaatat cctgcgccag ctcctcgacc tcgatggcgc
     8821 tgtctgtggg tccgccggtt atcataccat tcctgctctt tggcggcttc ttcttgaact
     8881 cgggctcggt gccagtatac ctcaaatggt tgtcgtacct ctcatggttc cgttacgcca
     8941 acgagggtct gctgattaac caatgggcgg acgtggagcc gggcgaaatt agctgcacat
     9001 cgtcgaacac cacgtgcccc agttcgggca aggtcatcct ggagacgctt aacttctccg
     9061 ccgccgatct gccgctggac tacgtgggtc tggccattct catcgtgagc ttccgggtgc
     9121 tcgcatatct ggctctaaga cttcgggccc gacgcaagga gtagccgaca tatatccgaa
     9181 ataactgctt gttttttttt ttaccattat taccatcgtg tttactgttt attgccccct
     9241 caaaaagcta atgtaattat atttgtgcca ataaaaacaa gatatgacct atagaataca
     9301 agtatttccc cttcgaacat ccccacaagt agactttgga tttgtcttct aaccaaaaga
     9361 cttacacacc tgcatacctt acatcaaaaa ctcgtttatc gctacataaa acaccgggat
     9421 atatttttta tatacatact tttcaaatcg cgcgccctct tcataattca cctccaccac
     9481 accacgtttc gtagttgctc tttcgctgtc tcccacccgc tctccgcaac acattcacct
     9541 tttgttcgac gaccttggag cgactgtcgt tagttccgcg cgattcggtt cgctcaaatg
     9601 gttccgagtg gttcatttcg tctcaataga aattagtaat aaatatttgt atgtacaatt
     9661 tatttgctcc aatatatttg tatatatttc cctcacagct atatttattc taatttaata
     9721 ttatgacttt ttaaggtaat tttttgtgac ctgttcggag tgattagcgt tacaatttga
     9781 actgaaagtg acatccagtg tttgttcctt gtgtagatgc atctcaaaaa aatggtgggc
     9841 ataatagtgt tgtttatata tatcaaaaat aacaactata ataataagaa tacatttaat
     9901 ttagaaaatg cttggatttc actggaacta gaattaattc ggctgctgct ctaaacgacg
     9961 catttcgtac tccaaagtac gaattttttc cctcaagctc ttattttcat taaacaatga
    10021 acaggaccta acgcacagtc acgttattgt ttacataaat gatttttttt actattcaaa
    10081 cttactctgt ttgtgtactc ccactggtat agccttcttt tatcttttct ggttcaggct
    10141 ctatcacttt actaggtacg gcatctgcgt tgagtcgcct ccttttaaat gtctgacctt
    10201 ttgcaggtgc agccttccac tgcgaatcat taaagtgggt atcacaaatt tgggagtttt
    10261 caccaaggct gcacccaagg ctctgctccc acaattttct cttaatagca cacttcggca
    10321 cgtgaattaa ttttactcca gtcacagctt tgcagcaaaa tttgcaatat ttcatttttt
    10381 tttattccac gtaagggtta atgttttcaa aaaaaaattc gtccgcacac aacctttcct
    10441 ctcaacaagc aaacgtgcac tgaatttaag tgtatacttc ggtaagcttc ggctatcgac
    10501 gggaccacct tatgttattt catcatg
//
